PerkinElmer

Reagents for Virology Research

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Viruses and virally transmitted diseases have been impacting human health and well-being for millennia. Some of our greatest scientific challenges and medical triumphs have involved virology, from smallpox to HIV and so many others. And today is no different. With the current outbreak of the novel coronavirus, SARS-CoV-2, and its corresponding viral disease, COVID-19, virology is once again in the spotlight.

PerkinElmer’s range of reagents offer support throughout the research and drug discovery process. Whether you are assessing stages of the viral life cycle or monitoring an antiviral immune response, our assay technologies provide simple, sensitive, streamlined workflows to help accelerate your viral studies.

Viral Life Cycle

From viral antigen and protein:protein interaction assays, to cytotoxicity and dsRNA kits, Alpha, HTRF®, and luminescence technologies offer solutions for identifying viral presence, attachment, and genome integration, as well as monitoring cytotoxicity and cell death.

Antiviral Immune Response

Part of developing immunity, and therefore a critical component to successful vaccination, is the body’s inflammatory response. As such, cytokines, inflammatory pathways and inflammatory mediators are important factors to look at when monitoring immunity. Pathways such as MAP kinase, JAK/STAT, and NFκB play a central role in the antiviral immune response along with ligands such as IL-6, TNFα, and the INFs (-γ, -α, -β, and -λ). PerkinElmer offers a wide range of Alpha and HTRF cytokine detection kits, phosphorylation detection kits, and toolbox reagents to monitor these critical indicators of the viral immune response.

COVID-19

Reportedly, a cytokine storm has been associated with COVID-19 infection1, leading to the rise in cytokine panel assays being used when studying the SARS-CoV-2 virus. These cytokine storms are the result of the body over-expressing multiple cytokines in a short period of time in an effort to protect itself from the virus, due to pro-inflammatory loops. This over inflammation is more harmful than helpful to the body. PerkinElmer offers Alpha and HTRF kits for the detection of IL-6, TNFα, and signaling proteins within the JAK/STAT pathway, all of which have shown to play an important role in this cytokine storm.

In addition to a range of cytokines, C-reactive protein (CRP) is also a protein of interest in the study of COVID-19. An inflammatory marker produced by the liver; CRP has been found to rise with the progression of COVID-19. PerkinElmer offers a no-wash, AlphaLISA® detection assay for the quantification of CRP in serum, buffered solution, or cell culture medium.

1Pedersen SF, Ho YC. SARS-CoV-2: a storm is raging. J Clin Invest. 2020 Apr 13. pii: 137647. doi: 10.1172/JCI137647. [Epub ahead of print]

100x100_AlphaLISA_general
100x100_AlphaLISA_general

Alpha Technology

Alpha is a homogeneous versatile, bead-based platform that enables you to assay even the most complex samples in one well and with no wash steps.

A range of AlphaLISA® detection kits are available for virology studies. Available kits allow for virus quantification, and cytokine detection. Specifically, the AlphaLISA IL-6 and C Reaction Protein (CRP) kits are of particular interest in looking at the COVID-19 cytokine storm. Additionally, AlphaLISA kits and Toolbox Acceptor Beads are available for immunoglobulin detection.

Alpha SureFire kits can be used to assess both innate and adaptive antiviral immunity. When assessing innate antiviral immunity a range of SureFire kits are available for proteins in the TLR, cGAS-STING, TLR/ cGAS-STING/ Inflammasome/ NF-kB, and JAK/STAT pathways. Kits are also available for assessing adaptative antiviral immunity, both through the T-cell activation pathway and the B-cell activation pathway.

100x100_HTRF_TR-FRET
100x100_HTRF_TR-FRET

HTRF

Cisbio’s HTRF® assays are fast, sensitive, homogeneous, and ready to use with no wash steps.

A range of HTRF kits are available for viral dsRNA and cytokine detection. Specifically, the HTRF IL-6 kit isof particular interest in looking at the COVID-19 cytokine storm.

HTRF assays can be used to assess both innate and adaptive antiviral immunity. When assessing innate antiviral immunity a range of HTRF assays are available for proteins in the TLR, cGAS-STING, TLR/ cGAS-STING/ Inflammasome/ NF-kB, and JAK/STAT pathways. Assays are also available for assessing adaptative antiviral immunity, both through the T-cell activation pathway and the B-cell activation pathway.

Furthermore, viral neutralization assays utilizing HTRF technology are also available.

Click here to learn more about the role of HTRF in virology research.

100x100_FireFlies_Cytotoxicity
100x100_FireFlies_Cytotoxicity

Luminescence Assays

Reporter gene, cytotoxicity, and cell proliferation assays are important tools not only in virology studies, but in drug discovery in general. Luminescence assays are ideal for use in viral neutralization assays and to monitor cell viability.

Luminescence detection platforms provide simple, reproducible results over a wide dynamic range. Our luciferase-based luminescence assays include reporter gene and ATP-monitoring assays.

100x100_OnPoint_2
100x100_OnPoint_2

Custom Assay Development

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha and HTRF assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

See Cisbio's customer development and labeling pages for more information on HTRF customization: https://www.cisbio.com/content/assay-developmenthttps://www.cisbio.com/content/custom-labeling.

 

HowCanWeHelp
HowCanWeHelp

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website http://www.perkinelmer.com/Alpha/ASKhttp://www.perkinelmer.com/SureFire/ASK .

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