PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

Products & Services (88)
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Application
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (41)
  • Protein Analysis & Detection (17)
  • Protein-protein interactions (19)
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  • LANCE (20)
  • LANCE Ultra (1)
  • LANCE SureLight (7)
  • LANCE Ultra (62)
Detection Method
  • Time-Resolved Fluorescence (TRF) (81)
  • TR-FRET (81)
Molecular Modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (39)

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1-25 of 88 Products & Services

  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 344.00 USD - 938.00 USD
  • LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
    Price Range : 1665.00 USD - 7700.00 USD
  • LANCE® Eu-W1024 Anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 1133.00 USD - 9900.00 USD
  • LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

    Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
    (mono-phosphorylated peptides at either residue will also be successfully recognized)
    Price Range : 2220.00 USD - 7700.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-HA

    Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.
    Price Range : 1133.00 USD - 15200.00 USD
  • LANCE Ultra ULight-CREBtide

    LANCE Ultra ULight™-CREBtide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 326.00 USD - 938.00 USD
  • LANCE® Eu-W1024 labeled anti-GST antibody

    Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 230.00 USD - 11300.00 USD
  • LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
    Price Range : 538.00 USD - 956.00 USD
  • LANCE Ultra ULight-anti-6xHis

    Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

    This is a replacement for TRF0105.

    Price Range : 488.00 USD - 12100.00 USD
  • LANCE Eu-W1024 Anti-6xHis

    LANCE Eu-W1024 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

    This is a replacement for AD0111.

    Price Range : 256.00 USD - 12100.00 USD
  • LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3860.00 USD - 9500.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-Human IgG

    Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 229.00 USD - 9900.00 USD
  • LANCE® Eu-W1024 phospho-threonine antibody, 10 µg

    Europium-labeled anti-phosphothreonine antibody, for use in LANCE® TR-FRET kinase assays. The antibody is an affinity purified rabbit polyclonal IgG.
    List Price : 1350.00 USD
  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 473.00 USD - 11500.00 USD
  • LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

    Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE
    Price Range : 2220.00 USD - 7700.00 USD
  • LANCE Ultra Europium-anti-phospho-CREB (Ser133)

    Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 1210.00 USD - 5200.00 USD
  • ULight-anti-V5 (LANCE TR-FRET)

    Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 451.00 USD - 11400.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Biotin

    Europium-labeled biotin for use in LANCE® TR-FRET assays.
    List Price : 1079.00 USD
  • LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641)

    Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL
    Price Range : 2587.00 USD - 7700.00 USD
  • ULight anti-human IgG

    ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
    Price Range : 465.00 USD - 11800.00 USD
  • LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

    Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9500.00 USD
  • LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236)

    Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA
    Price Range : 777.00 USD - 2690.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 251.00 USD - 910.00 USD
  • LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence: CKKSRGDYMTMQIG
    Price Range : 537.00 USD - 1303.00 USD
  • LANCE Ultra ULight™-poly GAT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 455.00 USD - 1041.00 USD
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