TR-FRET Toolbox Reagents

LANCE Eu-labeled antibody and SureLight-labeled antibody

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

Products & Services (88)
Technical Resources (76)
Tools & More (13)
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Products & Services (88)

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Application

Enzymatic (6)
Epigenetics (14)
Kinase (41)
Protein Analysis & Detection (17)
Protein-protein interactions (20)

Product Brand Name

LANCE (20)
LANCE Ultra (1)
LANCE SureLight (7)
LANCE Ultra (62)

Detection Method

Time-Resolved Fluorescence (TRF) (81)
TR-FRET (81)

Molecular Modification

Acetylation (6)
GlcNAc (1)
Methylation (9)
Phosphorylation (39)

Antibody Conjugates

Anti-6X His (3)
Anti-acetyl-lysine (1)
Anti-c-Myc (2)
Anti-C-tag (2)
Anti-FITC (2)
Anti-FLAG (3)
Anti-GST (3)
Anti-H3K27ac (1)
Anti-H3K27me2 (1)
Anti-H3K27me3 (1)
Anti-H3K36me2 (1)
Anti-H3K4 (1)
Anti-H3K4me1-2 (1)
Anti-H3K9/H3K27 (1)
Anti-H3K9ac (1)
Anti-H3K9me2 (1)
Anti-H4R3me (1)
Anti-HA (1)
Anti-human IgG (2)
Anti-methyl arginine (1)
Anti-mouse IgG (3)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (1)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-threonine (1)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
Anti-rabbit IgG (3)
Anti-V5 (2)
Biotin (1)
Protein A (1)
Protein G (1)
PT66 (1)
PY20 (1)
Streptavidin (5)

Fluorophore

Eu-W1024 (49)
Eu-W8044 (1)
SureLight APC (6)
SureLight-APC (1)
Ulight (1)
ULight (29)

Automation Compatible

Yes (88)

Experimental Type

In vitro (87)

Format

Microplates (86)

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1-25 of 88 Products & Services

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LANCE Eu-W1024 Anti-c-Myc

AD0206 , AD0115 , AD0114

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

Price Range : 324.00 USD - 15600.00 USD
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LANCE Ultra ULight™-anti-GST

TRF0104-M , TRF0104-D , TRF0104-R

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.

Price Range : 512.00 USD - 12100.00 USD
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ULight anti-c-myc antibody

TRF504M , TRF504R , TRF504D

ULight^™ labeled anti-c-myc antibody for LANCE^® TR-FRET assays.

Price Range : 465.00 USD - 11800.00 USD
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LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

TRF0407-M , TRF0407-D

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3787.00 USD - 9500.00 USD
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LANCE Ultra ULight-anti-6xHis

TRF0134-M , TRF0134-D , TRF0134-R

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.
This is a replacement for TRF0105.

Price Range : 488.00 USD - 12100.00 USD
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LANCE Eu-W1024 Anti-HA

AD0084 , AD0085

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.

Price Range : 1133.00 USD - 15200.00 USD
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LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

TRF0409-D , TRF0409-M

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3787.00 USD - 9500.00 USD
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LANCE^® Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody, 10 µg

AD0190

Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for use in LANCE® TR-FRET kinase assays.

List Price : 3695.00 USD
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LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

TRF0404-D , TRF0404-M

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3787.00 USD - 9500.00 USD
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LANCE Eu-W1024 Anti-6xHis

AD0110 , AD0111 , AD0205

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

Price Range : 256.00 USD - 12100.00 USD
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LANCE Ultra ULight™-MBP peptide

TRF0109-M , TRF0109-C , TRF0109-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight^™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.

Price Range : 344.00 USD - 938.00 USD
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LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody

TRF0211-C , TRF0211-M , TRF0211-D

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from Histone H3 phosphorylated at Thr3. Histone H3 peptides can be phosphorylated by several kinases including RSK2. Sequence motif recognized: ARTKQTARKSTGGKAPRKQLAGCG

Price Range : 1665.00 USD - 7700.00 USD
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LANCE Ultra ULight™-Protein A

TRF0103-D , TRF0103-M

Protein A labeled with ULight™ dye.

Price Range : 434.00 USD - 938.00 USD
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LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

TRF0213-D , TRF0213-M

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG

Price Range : 2371.00 USD - 7700.00 USD
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LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

TRF0130-M , TRF0130-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE

Price Range : 426.00 USD - 938.00 USD
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ULight anti-FITC

TRF503M , TRF503D , TRF503R

ULight™ labeled anti-FITC antibody for LANCE^® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.

Price Range : 465.00 USD - 11800.00 USD
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LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

TRF0203-C , TRF0203-M , TRF0203-D

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE

Price Range : 1665.00 USD - 7700.00 USD
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LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

TRF0125-C , TRF0125-M , TRF0125-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight^™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: CSERRPHFPQFSYSASGTAR

Price Range : 319.00 USD - 1303.00 USD
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ULight-anti-V5 (LANCE TR-FRET)

TRF505M , TRF505R , TRF505D

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.

Price Range : 451.00 USD - 11400.00 USD
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LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

TRF0111-M , TRF0111-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight^™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE

Price Range : 334.00 USD - 910.00 USD
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LANCE Ultra ULight™-Crosstide

TRF0106-M , TRF0106-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight^™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.

Price Range : 344.00 USD - 938.00 USD
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LANCE Ultra ULight™-poly GAT

TRF0101-D , TRF0101-M

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight^™ dye. This random polymer is a Tyrosine kinase substrate.

Price Range : 455.00 USD - 1041.00 USD
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LANCE Ultra ULight™-PKC substrate

TRF0108-D , TRF0108-M

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight^™ dye. Synthetic peptide derived from amino acids 19-31 of PKCα.

Price Range : 434.00 USD - 1184.00 USD
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LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

TRF0218-M , TRF0218-D

Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE

Price Range : 2220.00 USD - 7700.00 USD
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LANCE Eu-anti-C-tag antibody

AD0279 , AD0281 , AD0280

Europium-labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight™ APC-labeled reagents to create LANCE TR-FRET assays.

Price Range : 346.00 USD - 9800.00 USD

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