PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

Products & Services (82)
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  • LANCE (19)
  • LANCE Ultra (58)
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  • Time-Resolved Fluorescence (TRF) (75)
  • TR-FRET (75)
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1-25 of 82 Products & Services

  • LANCE Eu-W1024 Anti-6xHis

    LANCE Eu-W1024 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

    This is a replacement for AD0111.

    Price Range : 301.00 USD - 14200.00 USD
  • LANCE Ultra ULight-anti-6xHis

    Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

    This is a replacement for TRF0105.

    Price Range : 570.00 USD - 14200.00 USD
  • LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.
    Price Range : 532.00 USD - 1524.00 USD
  • ULight-anti-V5 (LANCE TR-FRET)

    Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 527.00 USD - 13300.00 USD
  • Europium-labeled antibody for LANCE assays

    Eu-anti-V5 (LANCE TR-FRET)

    Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 393.00 USD - 11100.00 USD
  • ULight anti-c-myc antibody

    ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
    Price Range : 544.00 USD - 13800.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 anti-FITC

    Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
    Price Range : 405.00 USD - 11300.00 USD
  • ULight anti-mouse IgG

    ULight™ labeled anti-mouse IgG antibody for LANCE® TR-FRET assays. The anti-mouse IgG antibody is a purified goat polyclonal antibody.
    Price Range : 544.00 USD - 13800.00 USD
  • ULight anti-rabbit IgG

    ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
    Price Range : 544.00 USD - 13800.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 anti-FLAG

    Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 511.00 USD - 11400.00 USD
  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 553.00 USD - 13400.00 USD
  • ULight anti-human IgG

    ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
    Price Range : 544.00 USD - 13800.00 USD
  • ULight anti-FITC

    ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
    Price Range : 544.00 USD - 13800.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
    Price Range : 391.00 USD - 1063.00 USD
  • LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
    Price Range : 498.00 USD - 1096.00 USD
  • LANCE Ultra ULight-TK peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.
    Price Range : 497.00 USD - 1463.00 USD
  • LANCE Ultra ULight™-Streptavidin

    Streptavidin labeled with ULight™ dye.
    Price Range : 402.00 USD - 7900.00 USD
  • LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
    Price Range : 629.00 USD - 1118.00 USD
  • LANCE Ultra ULight™-Protein A

    Protein A labeled with ULight™ dye.
    Price Range : 506.00 USD - 1096.00 USD
  • LANCE Ultra ULight™-poly GT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 402.00 USD - 1097.00 USD
  • LANCE Ultra ULight™-poly GAT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 532.00 USD - 1217.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 293.00 USD - 1063.00 USD
  • LANCE Ultra ULight™-PKC substrate

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight dye. Synthetic peptide derived from amino acids 19-31 of PKCα.
    Price Range : 508.00 USD - 1384.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight-p70 S6K (Thr389) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
    Price Range : 373.00 USD - 1524.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 402.00 USD - 1096.00 USD
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