PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

Products & Services (88)
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Application
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (41)
  • Protein Analysis & Detection (17)
  • Protein-protein interactions (19)
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  • LANCE (20)
  • LANCE Ultra (1)
  • LANCE SureLight (7)
  • LANCE Ultra (62)
Detection Method
  • Time-Resolved Fluorescence (TRF) (81)
  • TR-FRET (81)
Molecular Modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (39)

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1-25 of 88 Products & Services

  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: ARTKQTARKSTGGK
    Price Range : 333.00 USD - 1360.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 359.00 USD - 979.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

    Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3955.00 USD - 9900.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight-p70 S6K (Thr389) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
    Price Range : 333.00 USD - 1360.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Protein G

    Europium-labeled Protein G, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 303.00 USD - 10300.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Biotin

    Europium-labeled biotin for use in LANCE® TR-FRET assays.
    List Price : 1127.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 262.00 USD - 950.00 USD
  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 494.00 USD - 12000.00 USD
  • ULight anti-rabbit IgG

    ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
    Price Range : 486.00 USD - 12300.00 USD
  • LANCE® Eu-W1024 labeled anti-GST antibody

    Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 240.00 USD - 11800.00 USD
  • LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
    Price Range : 453.00 USD - 979.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-Mouse IgG

    Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 1183.00 USD - 10300.00 USD
  • PerkinElmer

    SureLight Allophycocyanin-anti-Rabbit antibody, 1 mg, lyophilized

    APC-labeled anti-rabbit IgG antibody, for capturing rabbit IgG antibodies in LANCE TR-FRET assays.
    List Price : 451.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
    Price Range : 349.00 USD - 950.00 USD
  • LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
    Price Range : 1739.00 USD - 8000.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

    Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
    Price Range : 1183.00 USD - 10300.00 USD
  • LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence: CKKSRGDYMTMQIG
    Price Range : 561.00 USD - 1361.00 USD
  • LANCE Ultra ULight™-Streptavidin

    Streptavidin labeled with ULight™ dye.
    Price Range : 359.00 USD - 7000.00 USD
  • LANCE Ultra ULight-CREBtide

    LANCE Ultra ULight™-CREBtide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 340.00 USD - 979.00 USD
  • ULight anti-FITC

    ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
    Price Range : 486.00 USD - 12300.00 USD
  • LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
    Price Range : 1739.00 USD - 8000.00 USD
  • LANCE Ultra ULight™-poly GT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 359.00 USD - 979.00 USD
  • LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone
    H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3955.00 USD - 9900.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 anti-FLAG

    Eu-W1024 labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 456.00 USD - 10200.00 USD
  • LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

    Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
    Price Range : 2476.00 USD - 8000.00 USD
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