PerkinElmer

TR-FRET Labeling Reagents

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Develop your own TR-FRET custom assays and label your biomolecule with a Europium lanthanide chelate. LANCE® Europium chelates use the following reactive groups for labeling chemistry:

  • Isothiocyanate (ITC)-activated chelates react primarily with free amine groups, including the N-terminus of a protein or peptide as well as lysine residues on antibodies, proteins, and peptides.
  • Iodoacetamide (IA)-activated chelates react with free sulfhydryl groups (including cysteines) on peptides and proteins to form a stable, covalent thio-ether bonds.
  • Diochlorotriazine (DTA)-activated chelates react with free amino, sulfhydryl, and hydroxyl groups and are suitable for labeling proteins, peptides, nucleic acids, and small molecules containing these functional groups.

LANCE Eu-W1024 chelates are preferred in most High Throughput Screening (HTS) applications due to their low fluorescent background and high S:B in TR-FRET assays. For assays that require high concentrations of EDTA or other chelators, a high temperature, or a pH below 7, the Eu-W8044 chelate offers greater stability.

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