PerkinElmer

Protein Kinase Research

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Cancer, diabetes, and Alzheimer’s research can benefit from the study of phosphorylation and protein kinase activity. Because of their role in signal transduction, kinase activity detection and characterization is significant for drug discovery.

We offer a range of end-point and real-time kinetic protein kinase assays, technologies, and instruments that allow rapid kinase detection, screening, and mechanism-of-action studies.

Our offerings include:

  • Labeled substrates for kinetic assays and antibody-free screening
  • AlphaLISA® kits for highly-sensitive detection of cellular phosphorylation
  • LANCE® TR-FRET protein kinase assays for rapid assay selection
  • Fast and simple assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Multimode plate readers for maximal speed and throughput independent of technology platform

Regulation and inhibition of kinase activity can open new pathways for therapeutics, especially in conditions related to cell signaling and growth factors.

Whether you work with serine/threonine-specific, tyrosine-specific, histidine-specific, or mixed kinases, our kinase activity assays and technologies enable rapid assay selection and accelerate drug development.

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  • Microplates (135)
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  • 40-50 (2)
  • 50-60 (2)

"Protein Kinase Research" 1-50 of 288 Products and Services

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EnSight Multimode Plate Reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1856.00 USD - 6000.00 USD
PerkinElmer

ProfilerPro Kinase Selectivity Assay Plate Kit 2, for LabChip EZ Reader

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.
List Price : 1559.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-Src (Tyr419) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 392.00 USD - 863.00 USD
AlphaLISA SureFire Ultra kits

Total SMAD2 AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ total SMAD2 assay is a sandwich immunoassay for quantitative detection of total SMAD2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-SMAD2.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT 1/2/3 (Thr308) kits are designed for the detection of phosphorylated AKT1, AKT2, and AKT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-MKK4 (Ser257) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated on Ser257) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-p38 MAPK Kit

The AlphaScreen® SureFire® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA SureFire Ultra kits

Total Cofilin AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra Total Cofilin assay is a sandwich immunoassay for quantitative detection of cofilin (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 489.00 USD - 29500.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 18, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 18 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 721.00 USD
AlphaLISA SureFire Ultra kits

Phospho-VEGFR2 (Tyr951) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-VEGFR2 assay is a sandwich immunoassay for quantitative detection of VEGFR (phosphorylated on Tyr951) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD3 (Ser423/425) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD3 (Ser423/425) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-IGF Receptor β (Tyr1135/1136) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-IGF Receptor β (Tyr1135/1136) assay is a sandwich immunoassay for quantitative detection of phospho-IGF Receptor β (phosphorylated on Tyr1135/1136) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 400.00 USD - 863.00 USD
SureFire principle

AlphaScreen SureFire STAT5 (p-Tyr694;Tyr699) Assay Kit 

The AlphaScreen® SureFire® p-STAT5 (p-Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD

ATP, [γ-32P]- 3000Ci/mmol 10mCi/ml EasyTide Lead

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
Price Range : 384.00 USD - 916.00 USD

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 180.00 USD - 13800.00 USD
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.
Price Range : 495.00 USD - 863.00 USD
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-MEK1 (Ser218/222) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

The AlphaScreen® SureFire® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 26, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 26 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 721.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-VEGFR2 (Tyr1214) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ phospho-VEGFR2 assay is a sandwich immunoassay for quantitative detection of VEGFR (phosphorylated on Tyr1214) in cellular lysates using Alpha Technology.
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD
PerkinElmer

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE
Price Range : 307.00 USD - 838.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total eIF2α LANCE Ultra TR-FRET Detection Kit

LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Insulin Receptor β (Tyr1150/1151) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-4E-BP1 (Thr37/46) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-4E-BP1 (Thr37/46) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
PerkinElmer

ProfilerPro Kinase Peptide Substrate 14, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 14 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 721.00 USD
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 1450.00 USD - 6500.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Tyr701) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Tyr701) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 29500.00 USD

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