operetta cls

Operetta CLS High Content Analysis System

Operetta® CLS is a high throughput microplate imager for high-content analysis (HCA). It can acquire, analyze and manage fluorescence, brightfield and digital phase contrast images. The combination of high power LED excitation with fast, precise mechanics and one large format sCMOS camera enables fast imaging. Controlled by Harmony software it offers a seamless workflow, for reliable discrimination of phenotypes even in complex cellular models.

To view our library of resources, images, and videos, visit the Resources and Image/Video Gallery sections below.

Features at a Glance

Intelligent image acquisition

Intelligent image acquisition

Target more accurately your object of interest for significantly reduced acquisition and analysis times, particularly valuable for 3D microtissue and rare event studies.

Machine learning

Machine learning

Easily create algorithms without being an image analysis expert using our PhenoLOGIC proprietary machine-learning technology.

Easily Quantify Cellular Phenotypes in Complex 3D Models

Easily quantify cellular phenotypes in complex 3D models

Explore your cell models by visualizing them in a 3D- and an XYZ-viewer and quantify volumetric and other 3D related phenotypic readouts.

Powerfully simple analysis capabilities

Powerfully simple analysis capabilities

Harmony® high-content imaging and analysis software - is the easy-to-learn, easy-to-use software that can make you more productive - faster.

High efficiency excitation design

High efficiency excitation design

Direct coupling limits the light loss that is typical of light guides or fibers.

Fast Frame Rate Imaging

Fast Frame Rate Imaging

Capture rapid cellular changes with an imaging frame rate up to 105 fps.

Automated water-immersion objectives

Automated water-immersion objectives

From the company with more than 20 years of experience - giving faster exposure times, increased resolution and reduced photodamage.

Confocal spinning-disk technology

Confocal spinning-disk technology

Provides a gentle imaging process (especially for live-cell experiments) with efficient background rejection.

Customer Testimonials

Dr. Davide Danovi
We have used the Operetta and Operetta CLS high-content devices from PerkinElmer extensively to quantitate cell behavior. It is important to combine an offer of intuitive off-the shelf solutions whilst allowing further analysis tools to be developed by users.
Dr. Davide Danovi
Centre for Stem Cells & Regenerative Medicine
King's College London
Dr. Doris Wilflingseder
Over the last few years, Operetta CLS has become increasingly important for our analysis and it provide lots of insights from a single transwell filter or from organoids in multiwell plates. Not only do you retrieve a lot of information, but you also get fantastic pictures. For example, we can visualize the interactions of SARS-CoV-2 with the multi-layered epithelia.
Univ. Prof. Dr. Doris Wilflingseder
Institute of Hygiene and Medical Microbiology
Medical University of Innsbruck

A Solution Configured to Suit Every Need

Whatever your application, there’s an Operetta CLS system configured to meet your requirements. And it’s modular, so it can change with your research demands.

Suit Every Need


Image/Video Gallery



Analysis of Mitochondrial Dynamics in Human iPSC-derived Neurons using the Operetta CLS High-Content Analysis System

Mitochondrial dynamics are essential for energy conversion and neuron survival. Understanding changes in mitochondrial dynamics is therefore crucial in developi ...
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Operetta CLS High-Content Analysis System Brochure

The product of more than a decade of experience in high-content screening, the Operetta CLS delivers the speed and sensitivity for everyday assays, and even com ...
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Cell Painting - A Cellular Imaging and Machine Learning Approach to Drug Discovery

Phenotypic Drug Discovery Re-Imagined Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combi ...
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Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ...
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Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large ...
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Publications for High-Content Screening

Publications in which PerkinElmer high-content analysis and screening systems are used number more than 1000 between 2010 and 2019. Here we feature some of the ...
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5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to t ...
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3D Volumetric and Zonal Analysis of Solid Spheroids

Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. Howe ...
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The Benefits of Water Immersion Lenses for High-Content Screening

The key parameters in high-content imaging - speed, sensitivity (or intensity) and resolution - cannot be optimized independently as altering one of them influe ...
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3D High-Content Screening of Organoids for Drug Discovery

This webinar will describe the marriage of 3D organoids and high-content screening (HCS) to discover targeted drug therapies effective against the malignant phe ...
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High Content Screening Applications

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