We provide innovative, tested, ready-to-go assays for your ever-changing detection needs.
Every day, our R&D scientists are busy developing fully validated, ready-to-use kits and reagents for the hottest new targets – the ones you’re working on now. Over the past couple of years, we’ve released over 300 new products, with plans to release even new ones every month.
The products below represent our newest assays. Be sure to check back frequently, as we’re constantly working to keep pace with the speed of your innovation to provide you with the products you need.
Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.
Ease-of-use: homogeneous, no-wash assay, with no separation steps
Fast: results in 3 hours for most applications
High sensitivity: detect down to femtogram levels of target
Large dynamic range: 4-5 logs
Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
Choose the Alpha solution that’s right for you
AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.
Homogeneous dispense-and-read technology
384-well and 1536-well formats
LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.
LANCE Ultra total STAT1 kits are designed for the detection of STAT1 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-STAT1 assay.
LANCE Ultra phospho-STAT1 (Tyr701) kits are designed for the detection of phosphorylated STAT1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.
The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Insulin Receptor β (Tyr1150/1151) in a multiplexed assay.
The AlphaLISA® immunoassay kit for human tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of human TNFR1 in buffered solution, serum, lysates, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
Hepatitis B Virus Surface antigen (HBsAg) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA HBsAg Detection Kit, but can also be ordered separately.
The Alpha SureFire®Ultra™ Multiplex phospho-STAT5 (Tyr694/695) + Total STAT5 assay kit is used to measure both the phosphorylation (on Tyr694/695) and total levels of endogenous STAT5 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT5 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.