Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

• Ease-of-use: homogeneous, no-wash assay, with no separation steps
• Fast: results in 3 hours for most applications
• High sensitivity: detect down to femtogram levels of target
• Large dynamic range: 4-5 logs
• Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

• AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
• AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
• AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
• Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

• No-wash/separation steps
• Homogeneous dispense-and-read technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.