In the race to the next big breakthrough you need to have the best tools available. We are constantly working to keep pace with the speed of your innovation to provide you with the products you need.
New targets are available in our AlphaLISA®, Alpha SureFire® Ultra™, and Alpha SureFire CETSA® assays in several different therapeutic areas.
New targets are also available in our HTRF® technology in the areas of cardiovascular, CNS, fibrosis, inflammation & auto-immunity, immuno-oncology, and oncology. See link under Suggested Content to learn more about HTRF and our other technologies with new targets available.
Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.
Choose the Alpha solution that’s right for you
DELFIA® (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) TRF technology combines the benefits of Time Resolved Fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DEFLIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.
DELFIA TRF offers benefits over traditional ELISA including:
From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.
From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.
LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.
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The AlphaLISA® SureFire® Ultra™ total Rb assay is a sandwich immunoassay for quantitative detection of Rb (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The Alpha SureFire® Ultra™ Multiplex phospho-PDGF Receptor β (Tyr751) + Total PDGF Receptor &beta assay kit is used to measure both the phosphorylation (on Tyr751) and total levels of endogenous PDGF Receptor β in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure PDGF Receptor β phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
The AlphaLISA® SureFire® Ultra™ total STAT6 assay is a sandwich immunoassay for quantitative detection of STAT6 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® SureFire® Ultra™ total STAT4 assay is a sandwich immunoassay for quantitative detection of STAT4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The Alpha SureFire® Ultra™ Multiplex phospho AMPKα1/2 (Thr172) + Total AMPKα1/2 assay kit is used to measure both the phosphorylation (on Thr172) and total levels of endogenous AMPKα1/2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure AMPKα1/2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
The Alpha SureFire® Ultra™ Multiplex phospho Cofilin (Ser3) + Total Cofilin assay kit is used to measure both the phosphorylation (on Ser3) and total levels of endogenous Cofilin in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Cofilin phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
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