PerkinElmer

LANCE TR-FRET Assays

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

LANCE Ultra Biomarker
LANCE Ultra Biomarker

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

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1-25 of 262 Products & Services

  • LANCE standard

    LANCE Ultra hIL-11, lyophilized (0.3ug)

    Human IL-11 standard, for use in running standard curves in LANCE® Ultra TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human IL-11 Detection Kit, but can be ordered separately.
    List Price : 312.00 USD
  • LANCE Eu-W1024 Anti-6xHis

    LANCE Eu-W1024 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

    This is a replacement for AD0111.

    Price Range : 301.00 USD - 14200.00 USD
  • LANCE Ultra ULight-anti-6xHis

    Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.

    This is a replacement for TRF0105.

    Price Range : 570.00 USD - 14200.00 USD
  • PerkinElmer

    MMP-10 (human), lyophilized (LANCE)

    Recombinant human MMP-10 standard, for use in LANCE Ultra TR-FRET quantitation assays. This standard is already included in the LANCE Ultra human MMP-10 detection kit, but can also be ordered separately.
    List Price : 925.00 USD
  • LANCE Ultra Biomarker

    MMP-10 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2930.00 USD - 13600.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra TBK1 Positive Control Lysate

    Control lysate for LANCE Ultra TR-FRET cellular phospho-TBK1 detection assays.
    List Price : 439.00 USD
  • Phospho-TBK1 (Ser172) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra STAT1 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-STAT1 and total STAT1 detection assays.
    List Price : 439.00 USD
  • Phospho-STAT1 (Tyr701) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-STAT1 (Tyr701) kits are designed for the detection of phosphorylated STAT1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra Biomarker

    Total STAT1 LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total STAT1 kits are designed for the detection of STAT1 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-STAT1 assay.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.
    Price Range : 532.00 USD - 1524.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra AMPKα Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-AMPKα detection assays.
    List Price : 439.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra LCK Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-LCK detection assays.
    List Price : 439.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra ZAP-70 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-ZAP-70 and Total ZAP-70 detection assays.
    List Price : 439.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra BTK Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-BTK and Total BTK detection assays.
    List Price : 439.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra NFκB Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-NFκB (Ser536) and Total NFκB detection assays.
    List Price : 439.00 USD
  • Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • Phospho-LCK (Tyr394) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra Biomarker

    Total eIF2α LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra Biomarker

    Total BTK LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total BTK kits are designed for the detection of total BTK (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-BTK detection, and is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • Phospho-BTK (Tyr223) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-BTK (Tyr223) kits are designed for the detection of phosphorylated BTK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra Biomarker

    Total NFκB LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
    Price Range : 1821.00 USD - 8200.00 USD
  • Phospho-NFκB (Ser536) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-NFκB (Ser536) kits are designed for the detection of phosphorylated NFκB in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1821.00 USD - 8200.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra Src Positive Control Lysate

    Control lysate for LANCE Ultra TR-FRET cellular phospho-Src detection assays.
    List Price : 439.00 USD
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