PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

Products & Services (358)
Filters:

Filters :

Assay Target
  • Adiponectin (2)
  • Akt1 (1)
  • Akt1/2/3 (2)
  • Albumin (2)
  • AMPKα (1)
  • ANGPT1 (2)
  • ANGPTL3 (2)
  • ANGPTL4 (2)
  • ApoA1 (2)
  • ApoC3 (2)
  • ApoE (2)
  • Aβ1-40 (2)
  • Bcl2 (2)
  • BTK (2)
  • c-Myc (2)
  • C-peptide (2)
  • cAMP (2)
  • CCL2 (3)
  • CCL3 (2)
  • CCL4 (2)
  • CCL5 (2)
  • CHO HCP (1)
  • CHO-P (1)
  • COL1A1 (2)
  • Cortisol (2)
  • CRP (2)
  • CXCL1 (2)
  • CXCL10 (2)
  • CXCL9 (2)
  • E-Cadherin (2)
  • E.coli HCP (2)
  • EGF (3)
  • EGFR (5)
  • eIF2α (1)
  • ErbB2 (2)
  • ERK1/2 (2)
  • FCGR3A (4)
  • Fibrinogen (2)
  • Fibronectin (2)
  • Frataxin (2)
  • FSH (2)
  • GAD67 (2)
  • GLP-1 (2)
  • Glucagon (2)
  • hCG (2)
  • IFN-λ2 (2)
  • IFNα (2)
  • IFNγ (4)
  • IgA (2)
  • IgE (2)
  • IGF1 (4)
  • IgG (8)
  • IgM (2)
  • IL-1RA (2)
  • IL-23A (2)
  • IL-31 (2)
  • IL11 (1)
  • IL12 (2)
  • IL17A (3)
  • IL17F (2)
  • IL1α (2)
  • IL1β (3)
  • IL2 (2)
  • IL28B (2)
  • IL4 (2)
  • IL5 (1)
  • IL6 (5)
  • IL8 (2)
  • Insulin (2)
  • Kappa light chain (2)
  • LC3B (2)
  • LCK (1)
  • Leptin (4)
  • MANF (2)
  • MBNL1 (1)
  • MIP1α (2)
  • MMP10 (2)
  • MMP12 (2)
  • MMP3 (2)
  • MMP7 (2)
  • MMP8 (2)
  • NFκB (2)
  • Nidogen-1 (2)
  • Oncostatin M (2)
  • p53 (2)
  • PAI-1 (2)
  • PCSK9 (1)
  • PD-1 (2)
  • PD-L1 (2)
  • PER.C6 HCP (2)
  • Perlecan (2)
  • PIP Collagen (2)
  • Protein A (2)
  • PTEN (2)
  • Residual DNA (2)
  • SLP-76 (2)
  • SMAD2 (1)
  • SMAD3 (2)
  • Src (2)
  • STAT1 (3)
  • STAT3 (2)
  • STAT5 (1)
  • Tau (2)
  • TBK1 (2)
  • TDP-43 (2)
  • TNFα (4)
  • tPA (1)
  • TREM2 (2)
  • TSH (2)
  • VEGF-A (4)
  • Zap70 (2)
Application
  • cAMP (2)
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (79)
  • Protein Analysis & Detection (234)
  • Protein-protein interactions (22)
Therapeutic Area
  • Angiogenesis (11)
  • Autophagy (2)
  • Biologics/Bioprocess (30)
  • Cancer (39)
  • Cardiovascular (1)
  • Central Nervous System (9)
  • CNS & Neurologic (1)
  • Inflammation (51)
  • Metabolic (36)
Product Brand Name
  • LANCE (27)
  • LANCE Ultra (49)
  • LANCE SureLight (7)
  • LANCE Ultra (251)
Detection Method
  • Time-Resolved Fluorescence (TRF) (314)
  • TR-FRET (314)
Molecular Modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (41)
Antibody Conjugates
  • Anti-6X His (3)
  • Anti-acetyl-lysine (1)
  • Anti-c-Myc (2)
  • Anti-C-tag (2)
  • Anti-FITC (2)
  • Anti-FLAG (3)
  • Anti-GST (3)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K4 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H4R3me (1)
  • Anti-HA (1)
  • Anti-human IgG (2)
  • Anti-methyl arginine (1)
  • Anti-mouse IgG (3)
  • Anti-O-linked-GlcNAc (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Tyr-100 (1)
  • Anti-p53K382ac (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-p70S6K (1)
  • Anti-phospho-PKC (1)
  • Anti-phospho-PLK (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-threonine (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-rabbit IgG (3)
  • Anti-V5 (2)
  • Biotin (1)
  • Protein A (1)
  • Protein G (1)
  • PT66 (1)
  • PY20 (1)
  • Streptavidin (5)
Fluorophore
  • Eu-W1024 (51)
  • Eu-W1024-ITC (2)
  • Eu-W1284-Iodoacetamido (2)
  • Eu-W8044 (1)
  • Eu-W8044-DTA (1)
  • SureLight APC (6)
  • SureLight-APC (1)
  • ULight (30)
Assay Points (200-50,000)
  • 10000 (126)
  • 500 (123)
  • 5000 (4)
Assay Target Class
  • Antibody (16)
  • Cytokine (53)
  • DNA (2)
  • Fc-receptor (4)
  • Hormone (16)
  • Peptide (10)
  • Phosphoprotein (19)
  • Protein (101)
  • Receptor (2)
Automation Compatible
  • Yes (348)
Experimental Type
  • In vitro (275)
Format
  • Microplates (279)

Sort by

1-25 of 358 Products & Services

  • Protein

    Apo-CIII (human), lyophilized (LANCE)

    Human Apo-CIII standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoC3 Detection Kit, but can also be ordered separately.
    List Price : 253.00 USD
  • LANCE Detection Kit

    IFN-γ (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra cAMP Detection Kit

    The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
    Price Range : 692.00 USD - 9000.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9400.00 USD
  • Protein

    IL-23A (human), lyophilized (LANCE)

    Human IL-23A standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-23A Detection Kit, but can also be ordered separately.
    List Price : 321.00 USD
  • LANCE Detection Kit

    IL-11 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • PerkinElmer

    SureLight Allophycocyanin-anti-FLAG antibody, 1 mg, lyophilized

    APC-labeled anti-FLAG antibody, for capturing FLAG-tagged proteins and peptides in LANCE TR-FRET assays.
    List Price : 619.00 USD
  • LANCE standard

    IFN-γ (mouse), lyophilized (LANCE)

    Mouse IFNγ standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Mouse IFNγ Detection Kit, but can also be ordered separately.
    List Price : 269.00 USD
  • Protein

    ANGPT1 (human), lyophilized (LANCE)

    Human angiopoietin 1 (ANGPT1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ANGPT1 Detection Kit, but can also be ordered separately.
    List Price : 261.00 USD
  • LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
    Price Range : 538.00 USD - 956.00 USD
  • LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
    Price Range : 1665.00 USD - 7700.00 USD
  • GLP-1 (7-36) (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human GLP-1 (7-36) Detection Kit is designed for detection and quantitation of human GLP-1 (7-36) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Detection Kit

    IL-23A (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-23A Detection Kit is designed for detection and quantitation of human interleukin-23A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Detection Kit

    IFN-α (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE® Eu-W1024 Anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 1133.00 USD - 9900.00 USD
  • LANCE Detection Kit

    GAD67 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • ANGPTL3 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ANGPTL3 Detection Kit is designed for detection and quantitation of human angiopoietin-like 3 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE standard

    LANCE Ultra m/rIL17A, lyophilized(1.0ug)

    LANCE Ultra m/rIL17A, lyophilized(1.0ug)
    List Price : 261.00 USD
  • AlphaLisa Analyte 2

    PIP Collagen (human), lyophilized (LANCE)

    PIP collagen standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra PIP Collagen TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 321.00 USD
  • LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
    Price Range : 2221.00 USD - 7700.00 USD
  • Glucagon (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Glucagon Detection Kit is designed for detection and quantitation of human glucagon in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Detection Kit

    IL-1β (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-1β Detection Kit is designed for detection and quantitation of human interleuukin-1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence: CKKSRGDYMTMQIG
    Price Range : 537.00 USD - 1303.00 USD
  • LANCE Ultra ULight™-CREBtide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 326.00 USD - 938.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total eIF2α LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • Jump to:
Business Insights (0)
Filters: