PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-25 of 363 Products & Services

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  • IL-4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-4 Detection Kit is designed for detection and quantitation of human interleukin 4 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 240.00 USD - 873.00 USD
  • PCSK9 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PCSK9 Detection Kit is designed for detection and quantitation of human Proprotein Convertase Subtilisin/Kexin Type 9 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
    Price Range : 320.00 USD - 873.00 USD
  • LANCE standard

    LANCE Ultra PCSK9, lyophilized (0.3ug)

    LANCE Ultra PCSK9, lyophilized (0.3ug)
    List Price : 250.00 USD
  • CCL5 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CCL5/RANTES Detection Kit is designed for detection and quantitation of human CCL5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Detection Kit

    PD-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PD-1 Detection Kit is designed for detection and quantitation of human PD-1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 453.00 USD - 11100.00 USD
  • IgM (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IgM Detection Kit is designed for detection and quantitation of human IgM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1494.00 USD - 9900.00 USD
  • Protein

    PD-1 (human), lyophilized (LANCE)

    Human PD-1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human PD-1 TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 308.00 USD
  • LANCE Ultra Lysis Buffer 1 (5X)

    LANCE Ultra Lysis Buffer 1 (5X)

    LANCE Ultra Lysis Buffer 1 is used in select LANCE Ultra cellular phosphophorylation detection kits. This buffer is already provided in these kits, but can be ordered separately.
    Price Range : 140.00 USD - 719.00 USD
  • CXCL1/GRO-α (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CXCL1/GRO-α Detection Kit is designed for detection and quantitation of human CXCL1 (GRO-α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • LANCE Detection Kit

    Residual Protein A LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • LANCE Detection Kit

    IL-5 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-5 Detection Kit is designed for detection and quantitation of human interleuukin-5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • LANCE Detection Kit

    Cortisol LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Cortisol Detection Kit is designed for detection and quantitation of cortisol in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1624.00 USD - 11100.00 USD
  • LANCE Detection Kit

    Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Detection Kit

    Tau (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human TAU Detection Kit is designed for detection and quantitation of human Tau in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-eIF2A (Ser51) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1512.00 USD - 6800.00 USD
  • LANCE Detection Kit

    IL-11 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • LANCE Detection Kit

    MBNL1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MBNL1 Detection Kit is designed for detection and quantitation of human muscleblind-like protein 1 (MBNL1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2405.00 USD - 11100.00 USD
  • IgE (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IgE Detection Kit is designed for detection and quantitation of human immunoglobulin E in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
    Price Range : 327.00 USD - 890.00 USD
  • IL-28B (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 930.00 USD - 12400.00 USD
  • PIP Collagen (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of human PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • LANCE Ultra ULight™-Protein A

    Protein A labeled with ULight™ dye.
    Price Range : 417.00 USD - 900.00 USD
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