PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

Products & Services (347)
Filters:
Assay Target
  • Adiponectin (2)
  • Akt1 (1)
  • Akt1/2/3 (2)
  • Albumin (2)
  • AMPKα (1)
  • ANGPT1 (2)
  • ANGPTL3 (2)
  • ANGPTL4 (2)
  • ApoA1 (2)
  • ApoC3 (2)
  • ApoE (2)
  • Aβ1-40 (2)
  • Bcl2 (2)
  • BTK (2)
  • c-Myc (2)
  • C-peptide (2)
  • cAMP (1)
  • CCL2 (3)
  • CCL3 (2)
  • CCL4 (2)
  • CCL5 (2)
  • COL1A1 (2)
  • Cortisol (2)
  • CRP (2)
  • CXCL1 (2)
  • CXCL10 (2)
  • CXCL9 (2)
  • E-Cadherin (2)
  • EGF (3)
  • EGFR (5)
  • eIF2α (1)
  • ErbB2 (2)
  • ERK1/2 (2)
  • FCGR3A (4)
  • Fibrinogen (2)
  • Fibronectin (2)
  • Frataxin (2)
  • FSH (2)
  • GAD67 (2)
  • GLP-1 (2)
  • hCG (2)
  • IFN-λ2 (2)
  • IFNα (2)
  • IFNγ (4)
  • IgA (2)
  • IgE (2)
  • IGF1 (4)
  • IgG (8)
  • IgM (2)
  • IL-1RA (2)
  • IL-23A (2)
  • IL-31 (2)
  • IL-7A (2)
  • IL11 (1)
  • IL12 (2)
  • IL17A (2)
  • IL17F (2)
  • IL1α (2)
  • IL1β (3)
  • IL2 (2)
  • IL28B (2)
  • IL4 (2)
  • IL5 (1)
  • IL6 (5)
  • IL8 (2)
  • Insulin (2)
  • Kappa light chain (2)
  • LC3B (2)
  • LCK (1)
  • Leptin (4)
  • MANF (2)
  • MBNL1 (1)
  • MIP1α (2)
  • MMP10 (2)
  • MMP12 (2)
  • MMP3 (2)
  • MMP7 (2)
  • MMP8 (2)
  • NFκB (2)
  • Nidogen-1 (2)
  • Oncostatin M (2)
  • p53 (2)
  • PAI-1 (2)
  • PCSK9 (1)
  • PD-1 (2)
  • PD-L1 (2)
  • Perlecan (2)
  • PIP Collagen (2)
  • Protein A (2)
  • PTEN (2)
  • Residual DNA (2)
  • SLP-76 (2)
  • SMAD2 (1)
  • SMAD3 (2)
  • Src (2)
  • STAT1 (3)
  • STAT3 (2)
  • STAT5 (1)
  • Tau (2)
  • TBK1 (2)
  • TDP-43 (2)
  • TNFα (4)
  • tPA (1)
  • TREM2 (2)
  • TSH (2)
  • VEGF-A (4)
  • Zap70 (2)
Application
  • cAMP (1)
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (79)
  • Protein Analysis & Detection (227)
  • Protein-protein interactions (20)
Assay Target Class
  • Antibody (16)
  • Cytokine (54)
  • DNA (2)
  • Fc-receptor (4)
  • Hormone (14)
  • Peptide (10)
  • Phosphoprotein (19)
  • Protein (95)
  • Receptor (2)
Therapeutic Area
  • Angiogenesis (11)
  • Autophagy (2)
  • Biologics/Bioprocess (24)
  • Cancer (39)
  • Cardiovascular (1)
  • Central Nervous System (9)
  • CNS & Neurologic (1)
  • Inflammation (52)
  • Metabolic (34)
Product Brand Name
  • LANCE (26)
  • LANCE Ultra (49)
  • LANCE SureLight (7)
  • LANCE Ultra (242)
Detection Method
  • Time-Resolved Fluorescence (TRF) (307)
  • TR-FRET (307)
Molecular Modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (41)

Sort by

1-25 of 347 Products & Services

  • LANCE Ultra Biomarker

    MMP-10 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2617.00 USD - 12100.00 USD
  • AlphaLisa Analyte 2

    MMP-7 (human), lyophilized (LANCE)

    Human MMP-7 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human MMP-7 TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 322.00 USD
  • LANCE Ultra Biomarker

    Kappa Light Chain (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1012.00 USD - 13500.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra Biomarker

    BCL-2 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human BCL-2 Detection Kit is designed for detection and quantitation of human Bcl-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE standard

    LANCE Ultra Mouse/Rat IL-17A, lyophilized. 1.0 μg

    Mouse IL-17A standard, for use in running standard curves in LANCE® Ultra™ TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Mouse IL-17A Detection Kit, but can be ordered separately.

    List Price : 273.00 USD
  • LANCE Ultra Biomarker

    IL-17A (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-17A Detection Kit is designed for detection and quantitation of human IL-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3955.00 USD - 9800.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 359.00 USD - 979.00 USD
  • LANCE Ultra Biomarker

    Albumin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-LCK (Tyr394) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • PerkinElmer

    IGF-1 (rat), lyophilized (LANCE)

    Rat IGF-1 (insulin-like growth factor 1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra rat IGF-1 Detection Kit, but can also be ordered separately.
    List Price : 335.00 USD
  • PerkinElmer

    CCL4 (human), lyophilized (LANCE)

    Human CCL4/MIP-1ß standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human CCL4/MIP-1ß TR-FRET Detection Kit, but can be ordered separately.
    List Price : 273.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-anti-C-tag antibody

    Europium-labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight™ APC-labeled reagents to create LANCE TR-FRET assays.
    Price Range : 361.00 USD - 10200.00 USD
  • LANCE Ultra Biomarker

    TSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • PerkinElmer

    BCL-2 (human), lyophilized (LANCE)

    Human BCL-2 standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET Detection Assays. This standard is already provided in the LANCE Ultra Human Bcl-2 Detection Kit, but can be ordered separately.
    List Price : 346.00 USD
  • PerkinElmer

    Perlecan (human), lyophilized (LANCE)

    Human perlecan standard, for use in running standard curves in LANCE Ultra TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human Perlecan Detection Kit, but can also be ordered separately.
    List Price : 346.00 USD
  • LANCE Ultra Biomarker

    ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: ARTKQTARKSTGGK
    Price Range : 333.00 USD - 1360.00 USD
  • LANCE Ultra Biomarker

    IFN-γ (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IFNγ Detection Kit is designed for detection and quantitation of human interferon gamma in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE standard

    IL-6 (human), lyophilized (LANCE), 0.1 µg

    Human Interleukin 6 (IL-6) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-6 Detection Kit, but can be ordered separately.
    List Price : 273.00 USD
  • LANCE Ultra Biomarker

    Total BTK LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total BTK kits are designed for the detection of total BTK (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-BTK detection, and is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • PerkinElmer

    Cortisol, liquid (LANCE)

    Cortisol standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Cortisol Detection Kit, but can also be ordered separately.
    List Price : 851.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra LCK Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-LCK detection assays.
    List Price : 392.00 USD
  • LANCE Ultra Biomarker

    Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • Jump to:
Business Insights (0)
Filters: