PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-12 of 363 Products & Services

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  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-LCK (Tyr394) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1512.00 USD - 6800.00 USD
  • LANCE Detection Kit

    Oncostatin M (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Oncostatin M Detection Kit is designed for detection and quantitation of human OSM in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • Protein

    Oncostatin M (human), lyophilized (LANCE)

    Human Oncostatin M (OSM) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET Detection Assays. This standard is already provided in the LANCE Ultra Human Oncostatin M Detection Kit, but can be ordered separately.
    List Price : 317.00 USD
  • LANCE Detection Kit

    PD-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PD-1 Detection Kit is designed for detection and quantitation of human PD-1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total ZAP-70 LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total ZAP-70 kits are designed for the detection of ZAP-70 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-ZAP-70 assay.
    Price Range : 1512.00 USD - 6800.00 USD
  • Residual Protein A LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • Protein

    Fibronectin (human), lyophilized (LANCE)

    Human fibronectin standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human Fibronectin Detection Kit, but can be ordered separately.
    List Price : 317.00 USD
  • Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • IL-11 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • ULight anti-rabbit IgG

    ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
    Price Range : 446.00 USD - 11300.00 USD
  • LANCE Detection Kit

    EGF-EGFR LANCE Ultra TR-FRET Binding Kit

    This kit is designed for the detection of binding activity between Human EGF and EGFR using a homogeneous LANCE Ultra TR-FRET assay. This assay can facilitate the design and development of therapeutics which competitively inhibit EGF/EGFR interaction.
    Price Range : 806.00 USD - 10700.00 USD
  • LANCE Detection Kit

    EGFR (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
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