PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

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1-25 of 350 Products & Services

  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-STAT5 (Tyr694/699) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-STAT5 (Tyr694/699) kits are designed for the detection of STAT5 (phosphorylated at Y694/Y699) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra EGFR Positive Control Lysate

    Control lysate for LANCE Ultra TR-FRET cellular phospho-EGFR and Total EGFR detection assays.
    List Price : 376.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra eIF2α Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-eIF2a detection assays.
    List Price : 376.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 344.00 USD - 938.00 USD
  • LANCE standard

    IFNα (human), lyophilized (LANCE)

    Human IFNα standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IFNα Detection Kit, but can also be ordered separately.
    List Price : 269.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total EGFR LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra total EGFR kits are designed for the detection of EGFR (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-EGFR, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 251.00 USD - 910.00 USD
  • Protein

    p53 (human), lyophilized (LANCE)

    Human p53 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra p53 Detection Kit, but can also be ordered separately.
    List Price : 1217.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • Protein

    Host Cell Residual DNA standard (LANCE)

    DNA standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Residual DNA Detection Kit, but can also be ordered separately.
    List Price : 321.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-SMAD2 (Ser465/467) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SMAD2 (Ser465/467) kits are designed for the detection of SMAD2 (phosphorylated at S465/S467) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Detection Kit

    IL-11 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Detection Kit

    IL17F (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-17F Detection Kit is designed for detection and quantitation of human interleukin 17 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Protein

    Apo-CIII (human), lyophilized (LANCE)

    Human Apo-CIII standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra ApoC3 Detection Kit, but can also be ordered separately.
    List Price : 253.00 USD
  • LANCE Detection Kit

    Fibronectin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Fibronectin Detection Kit is designed for detection and quantitation of human fibronectin in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Protein

    MMP-12 (human), lyophilized (LANCE)

    Human MMP-12 standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra MMP-12 TR-FRET Detection Kit, but can be ordered separately.
    List Price : 331.00 USD
  • LANCE Detection Kit

    LC3B (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human microtubule-associated protein 1 light chain 3 isoform B (LC3B) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • Protein

    Oncostatin M (human), lyophilized (LANCE)

    Human Oncostatin M (OSM) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET Detection Assays. This standard is already provided in the LANCE Ultra Human Oncostatin M Detection Kit, but can be ordered separately.
    List Price : 331.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ultra cAMP Detection Kit

    The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
    Price Range : 692.00 USD - 9000.00 USD
  • Protein

    Albumin (human), lyophilized (LANCE)

    Human albumin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Albumin TR-FRET Detection Kit, but can be ordered separately.
    List Price : 261.00 USD
  • LANCE Detection Kit

    EGFR (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Detection Kit

    FSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
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