PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-12 of 363 Products & Services

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  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-AKT 1 (Thr308) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-AKT1 (Thr308) kits are designed for the detection of AKT1 (phosphorylated at T308) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1512.00 USD - 6800.00 USD
  • Protein

    ANGPTL3 (human), lyophilized (LANCE)

    Human angiopoietin-like 3 (ANGPTL3) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ANGPTL3 Detection Kit, but can also be ordered separately.
    List Price : 250.00 USD
  • Protein

    Adiponectin (human), lyophilized (LANCE)

    Human adiponectin standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Adiponectin TR-FRET Detection Kit, but can be ordered separately.
    List Price : 250.00 USD
  • Protein

    COX-2 (human), liquid (LANCE)

    Human COX-2 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COX-2 TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 308.00 USD
  • LANCE Ultra ULight™-poly GAT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 436.00 USD - 999.00 USD
  • LANCE Detection Kit

    IL-8 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-8 Detection Kit is designed for detection and quantitation of human interleukin 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • Protein

    ANGPT1 (human), lyophilized (LANCE)

    Human angiopoietin 1 (ANGPT1) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human ANGPT1 Detection Kit, but can also be ordered separately.
    List Price : 250.00 USD
  • LANCE Detection Kit

    Adiponectin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Detection Kit

    MMP-7 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-7 Detection Kit is designed for detection and quantitation of human MMP-7 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 806.00 USD - 10700.00 USD
  • LANCE Detection Kit

    MMP-3 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-3 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase-3 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • IL-6 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • LANCE Ultra ULight-CREBtide

    LANCE Ultra ULight™-CREBtide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 313.00 USD - 900.00 USD
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