PerkinElmer

LANCE TR-FRET

OF

LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

Assays for Biomarker Detection
Assays for Biomarker Detection

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. http://www.perkinelmer.com/LANCE/ASK.

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1-25 of 352 Products & Services

  • IL-1α (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-1α Detection Kit is designed for detection and quantitation of human interleuukin-1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total EGFR LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra total EGFR kits are designed for the detection of EGFR (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-EGFR, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE standard

    IL-1α (human), lyophilized (LANCE)

    Human IL-1α standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-1α Detection Kit, but can also be ordered separately.
    List Price : 269.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra EGFR Positive Control Lysate

    Control lysate for LANCE Ultra TR-FRET cellular phospho-EGFR and Total EGFR detection assays.
    List Price : 376.00 USD
  • LANCE Ultra ULight-CREBtide

    LANCE Ultra ULight™-CREBtide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 326.00 USD - 938.00 USD
  • IL-11 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-11 Detection Kit is designed for detection and quantitation of human interleukin-11 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

    Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
    Price Range : 1133.00 USD - 9900.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-Src (Tyr419) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-Src (Tyr419) kits are designed for the detection of Src (phosphorylated at residue Y419) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE standard

    Protein A, lyophilized (LANCE)

    Protein A standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Residual Protein A Detection Kit, but can also be ordered separately.
    List Price : 261.00 USD
  • GAD67 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra eIF2α Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-eIF2a detection assays.
    List Price : 376.00 USD
  • LANCE Ultra ULight™-PKC substrate

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight dye. Synthetic peptide derived from amino acids 19-31 of PKCα.
    Price Range : 434.00 USD - 1184.00 USD
  • CCL5 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CCL5/RANTES Detection Kit is designed for detection and quantitation of human CCL5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • CCL2/MCP-1α (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse CCL2/MCP-1α Detection Kit is designed for detection and quantitation of mouse Monocyte Chemoattractant Protein 1α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
    Price Range : 1665.00 USD - 7700.00 USD
  • Protein

    CCL3/MIP1α (human), lyophilized (LANCE)

    Human CCL3 (MIP1α) standard, lyophilized, for use in running standard curves in LANCE TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human CCL3/MIP1α Detection Kit, but can be ordered separately.
    List Price : 261.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra LCK Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-LCK detection assays.
    List Price : 376.00 USD
  • LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

    Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
    Price Range : 234.00 USD - 10600.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total NFκB LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
    Price Range : 1557.00 USD - 7000.00 USD
  • IFN-α (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • CCL4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CCL4/MIP-1ß Detection Kit is designed for detection and quantitation of human C-C Motif Chemokine 4/Macrophage Inflammatory Protein-1beta in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9400.00 USD
  • LANCE Ultra Europium anti-phospho-Glycogen Synthase (Ser641)

    Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL
    Price Range : 2587.00 USD - 7700.00 USD
  • LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
    Price Range : 341.00 USD - 928.00 USD
  • PerkinElmer

    LANCE® Eu-W1024 labeled anti-GST antibody

    Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 230.00 USD - 11300.00 USD
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