PerkinElmer

LANCE TR-FRET

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LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

Products & Services (348)
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1-25 of 348 Products & Services

  • LANCE Ultra ULight™-Streptavidin

    Streptavidin labeled with ULight™ dye.
    Price Range : 344.00 USD - 6700.00 USD
  • LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

    Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
    Price Range : 2221.00 USD - 7700.00 USD
  • LANCE® Eu-W1024 phospho-threonine antibody, 10 µg

    Europium-labeled anti-phosphothreonine antibody, for use in LANCE® TR-FRET kinase assays. The antibody is an affinity purified rabbit polyclonal IgG.
    List Price : 1350.00 USD
  • Europium-labeled antibody for LANCE assays

    Eu-anti-V5 (LANCE TR-FRET)

    Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
    Price Range : 336.00 USD - 9500.00 USD
  • ULight anti-rabbit IgG

    ULight™ labeled anti-rabbit IgG antibody for LANCE® TR-FRET assays. The anti-rabbit IgG antibody is a purified mouse polyclonal antibody.
    Price Range : 465.00 USD - 11800.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ultra Biomarker

    CRP (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Human CRP Detection Kit is designed for detection and quantitation of human C-reactive protein in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    MMP-3 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-3 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase-3 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    Nidogen 1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Nidogen 1 Detection Kit is designed for detection and quantitation of Nidogen 1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody

    Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.
    Price Range : 3787.00 USD - 9400.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra AMPKα Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-AMPKα detection assays.
    List Price : 376.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-LCK (Tyr394) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ultra Biomarker

    CXCL1/GRO-α (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CXCL1/GRO-α Detection Kit is designed for detection and quantitation of human CXCL1 (GRO-α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

    Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
    Price Range : 234.00 USD - 10600.00 USD
  • LANCE Ultra Biomarker

    IL-2 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-2 Detection Kit is designed for detection and quantitation of human IL-2 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. It is a fast and simple alternative to a human IL-2 ELISA kit.
    Price Range : 865.00 USD - 11500.00 USD
  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 473.00 USD - 11500.00 USD
  • LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342)

    Europium-labeled mouse monoclonal antibody recognizing human DNA Topoisomerase 2-alpha phosphorylated at Thr1342. Sequence motif recognized: DEK(pT)DDE
    Price Range : 2220.00 USD - 7700.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 251.00 USD - 910.00 USD
  • LANCE Ultra Biomarker

    LC3B (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human microtubule-associated protein 1 light chain 3 isoform B (LC3B) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Ultra Biomarker

    PD-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PD-1 Detection Kit is designed for detection and quantitation of human PD-1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra Biomarker

    Total BTK LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total BTK kits are designed for the detection of total BTK (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-BTK detection, and is compatible with both adherent and suspension cells.
    Price Range : 1557.00 USD - 7000.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 344.00 USD - 938.00 USD
  • LANCE Ultra Biomarker

    E cadherin (human) LANCE Ultra TR-FRET Detection Kit

    Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.
    Price Range : 840.00 USD - 11200.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra SMAD1 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-SMAD1 detection assays.
    List Price : 376.00 USD
  • PerkinElmer

    COL1A1 (human), lyophilized (LANCE)

    Human COL1A1 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human COL1A1 TR-FRET Detection Kit, but can also be ordered separately.
    List Price : 321.00 USD
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