PerkinElmer

LANCE TR-FRET

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LANCE® (Lanthanide Chelate Excite) TR-FRET technology combines the benefits of Time Resolved Fluorescence (TRF) with those of Förster Resonance Energy Transfer (FRET) in a simple, highly-sensitive, and highly reproducible assay platform for detection, quantitation and screening in microplate format. We now also offer PerkinElmer Cisbio HTRF® assay technology. HTRF (Homogeneous Time Resolved Technology), allowing us to provide a wide range of assays kits in a homogenous TR-FRET technology.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. Furthermore, FRET detection utilizes energy donor and energy acceptor fluorophore labels in solution to create a no-wash detection assay. By combining the benefits of TRF and FRET, LANCE TR-FRET offers a multitude of benefits over standard fluorometric detection:

  • Homogeneous assay format (no wash steps, no separation steps)
  • Reduced background leading to better S:B
  • Large Stoke’s shift
  • Wider dynamic range
  • Extended signal stability
  • Increased sensitivity. Lower detection limits of 10 pg/mL compared to 100 pg/mL for standard fluorometric detection
  • Faster time to results

From biomarker detection assays, to cAMP/GPCR studies, to kinase and epigenetic assays, our LANCE® and LANCE Ultra TR-FRET assays are sure to save you time and hassle.

HTRF_84458_320x320f
HTRF_84458_320x320f

HTRF (Homogeneous Time Resolved Technology)

 

We now also offer PerkinElmer Cisbio HTRF® assay technology. Find out more about HTRF (Homogeneous Time Resolved Technology) and the products offered in this technology here.

Assays for Biomarker Detection

Biomarker detection is key for advancing precision medicine. An array of biomarker detection kits are available utilizing our LANCE technologies in therapeutic areas including:
  • Angiogenesis
  • Animal Health
  • Autophagy
  • Biologics/Bioprocess
  • Cancer
  • Cardiovascular
  • Central nervous system
  • Inflammation
  • Metabolic
OnPoint Services
OnPoint Services

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website. https://www.perkinelmer.com/LANCE/ASK.

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1-25 of 341 Products & Services

  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 359.00 USD - 979.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 262.00 USD - 950.00 USD
  • LANCE Ultra Biomarker

    PAI-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PAI-1 Detection Kit is designed for detection and quantitation of human PAI-1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra ULight™-Protein A

    Protein A labeled with ULight™ dye.
    Price Range : 452.00 USD - 979.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
    Price Range : 349.00 USD - 950.00 USD
  • LANCE Ultra Biomarker

    p53 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra p53 Detection Kit is designed for detection and quantitation of human p53 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2541.00 USD - 11700.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3955.00 USD - 9800.00 USD
  • LANCE Ultra Biomarker

    Insulin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Insulin Detection Kit is designed for detection and quantitation of human insulin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra control lysate

    LANCE Ultra ZAP-70 Positive Control Lysate

    Control lysates for LANCE Ultra TR-FRET cellular phospho-ZAP-70 and Total ZAP-70 detection assays.
    List Price : 392.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: ARTKQTARKSTGGK
    Price Range : 333.00 USD - 1360.00 USD
  • LANCE Ultra Biomarker

    CCL5 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human CCL5/RANTES Detection Kit is designed for detection and quantitation of human CCL5 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
    Price Range : 2318.00 USD - 8000.00 USD
  • LANCE Ultra ULight™-IkappaB-alpha (Ser32/36) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser32/36 of human IkappaB-alpha. The ULight-IkappaB-alpha peptide can be phosphorylated at Ser 32/36 by the IKKβ kinase. Peptide core motif: RHDSGLDSM
    Price Range : 356.00 USD - 969.00 USD
  • LANCE Ultra Europium-anti-phospho-CREB (Ser133)

    Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 1264.00 USD - 5400.00 USD
  • PerkinElmer

    Ultra HiBlock Buffer 5X

    This product is designed to be used in combination with LANCE Ultra TR-FRET biomarker detection kits. The Ultra HiBlock buffer was specially developed buffer to block non-specific protein binding sites.
    Price Range : 257.00 USD - 488.00 USD
  • PerkinElmer

    Oncostatin M (human), lyophilized (LANCE)

    Human Oncostatin M (OSM) standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET Detection Assays. This standard is already provided in the LANCE Ultra Human Oncostatin M Detection Kit, but can be ordered separately.
    List Price : 346.00 USD
  • LANCE® Eu-W1024 Anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG, for capturing antibodies in LANCE® TR-FRET assays.
    Price Range : 1183.00 USD - 10300.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight-p70 S6K (Thr389) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
    Price Range : 333.00 USD - 1360.00 USD
  • LANCE Ultra ULight™-poly GT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 359.00 USD - 979.00 USD
  • LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36)

    Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from IkappaB-alpha phosphorylated at Ser32/36. IkappaB-alpha (Ser32/36) peptides can be phosphorylated by the IKKβ kinase. Sequence motif recognized: RHDSGLDSM
    Price Range : 1636.00 USD - 5300.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • PerkinElmer

    p53 (human), lyophilized (LANCE)

    Human p53 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra p53 Detection Kit, but can also be ordered separately.
    List Price : 1271.00 USD
  • LANCE Ultra Biomarker

    TNFα (mouse) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Mouse TNFα Detection Kit is designed for detection and quantitation of mouse tumor necrosis factor α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
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