PerkinElmer

LANCE TR-FRET Immunoassays

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ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

LANCE® (Lanthanide Chelate Excite) TR-FRET is a homogeneous (no-wash) alternative to traditional ELISA. LANCE is a proximity assay that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with faster time to results and no wash steps.

 

In a LANCE TR-FRET immunoassay, a target specific antibody is either conjugated directly to or is captured by an antibody conjugated to a Europium chelate. A second, target specific, antibody is either conjugated to or is captured by an antibody conjugated to the ULight™ acceptor dye. These two target- specific antibody capture the analyte, brining the Europium chelate donor and the ULight acceptor dye into proximity. Upon excitation at 320 nm or 340 nm, the Europium chelate is excited and energy is transferred to the ULight dye, which emits light at 665 and 615 nm. The amount of light emitted is proportional to the amount of analyte present in the sample.

LANCE TR-FRET offers benefits over traditional ELISA and other immunodetection technologies including:

  • No-wash
  • Streamlined protocol with one or 2 additional steps
  • Faster time to results
  • Miniaturizable
  • Wider dynamic range, up to 4 log
  • Longer signal stability

For research use only. Not for use in diagnostic procedures.

 

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100x100_NewestAssays

Make the switch to LANCE TR-FRET

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 181 Products & Services

  • LANCE Ultra Biomarker

    MMP-10 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-10 Detection Kit is designed for detection and quantitation of human MMP-10 in cell culture media and cell supernatants using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 2617.00 USD - 12100.00 USD
  • LANCE Ultra Biomarker

    Kappa Light Chain (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Kappa Light Chain Detection Kit is designed for detection and quantitation of human kappa light chain in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1012.00 USD - 13500.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra Biomarker

    BCL-2 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human BCL-2 Detection Kit is designed for detection and quantitation of human Bcl-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    IL-17A (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-17A Detection Kit is designed for detection and quantitation of human IL-17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3955.00 USD - 9800.00 USD
  • LANCE Ultra Biomarker

    Albumin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Albumin Detection Kit is designed for detection and quantitation of human albumin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Phopho-protein

    Phospho-LCK (Tyr394) LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra phospho-LCK (Tyr394) kits are designed for the detection of phosphorylated LCK in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-anti-C-tag antibody

    Europium-labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE ULight or SureLight™ APC-labeled reagents to create LANCE TR-FRET assays.
    Price Range : 361.00 USD - 10200.00 USD
  • LANCE Ultra Biomarker

    TSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human TSH Detection Kit is designed for detection and quantitation of human thyroid stimulating hormone in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    IFN-γ (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IFNγ Detection Kit is designed for detection and quantitation of human interferon gamma in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    Total BTK LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total BTK kits are designed for the detection of total BTK (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-BTK detection, and is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra Biomarker

    Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    IGF-1 (rat) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Rat IGF-1 Detection Kit is designed for detection and quantitation of rat insulin-like growth factor 1 (IGF-1) in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 877.00 USD - 11700.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-c-Myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 338.00 USD - 16300.00 USD
  • LANCE Ultra Biomarker

    LC3B (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human microtubule-associated protein 1 light chain 3 isoform B (LC3B) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    MANF (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MANF Detection Kit is designed for detection and quantitation of human MANF in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 877.00 USD - 11700.00 USD
  • LANCE Ultra Biomarker

    IFN-λ2 (human) LANCE TR-FRET Detection Kit

    The LANCE® Ultra Human IFN-λ2 Detection Kit is designed for detection and quantitation of human interferon λ2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 1768.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    Perlecan (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Perlecan Detection Kit is designed for detection and quantitation of human perlecan in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • LANCE Ultra Biomarker

    IL-1β (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-1β Detection Kit is designed for detection and quantitation of human interleuukin-1β in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 903.00 USD - 12000.00 USD
  • PerkinElmer

    MMP-10 (human), lyophilized (LANCE)

    Recombinant human MMP-10 standard, for use in LANCE Ultra TR-FRET quantitation assays. This standard is already included in the LANCE Ultra human MMP-10 detection kit, but can also be ordered separately.
    List Price : 826.00 USD
  • LANCE Ultra Biomarker

    FSH (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Follicle Stimulating Hormone (FSH) Detection Kit is designed for detection and quantitation of human FSH in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 877.00 USD - 11700.00 USD
  • LANCE Ultra Biomarker

    Total ZAP-70 LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total ZAP-70 kits are designed for the detection of ZAP-70 (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-ZAP-70 assay.
    Price Range : 1626.00 USD - 7300.00 USD
  • LANCE Ultra Biomarker

    Total eIF2α LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra Total eIF2α kits are designed for the detection of total eIF2α (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-eIF2α detection, and is compatible with both adherent and suspension cells.
    Price Range : 1626.00 USD - 7300.00 USD
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