PerkinElmer

LANCE TR-FRET Immunoassays

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ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

LANCE® (Lanthanide Chelate Excite) TR-FRET is a homogeneous (no-wash) alternative to traditional ELISA. LANCE is a proximity assay that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with faster time to results and no wash steps.

 

In a LANCE TR-FRET immunoassay, a target specific antibody is either conjugated directly to or is captured by an antibody conjugated to a Europium chelate. A second, target specific, antibody is either conjugated to or is captured by an antibody conjugated to the ULight™ acceptor dye. These two target- specific antibody capture the analyte, brining the Europium chelate donor and the ULight acceptor dye into proximity. Upon excitation at 320 nm or 340 nm, the Europium chelate is excited and energy is transferred to the ULight dye, which emits light at 665 and 615 nm. The amount of light emitted is proportional to the amount of analyte present in the sample.

LANCE TR-FRET offers benefits over traditional ELISA and other immunodetection technologies including:

  • No-wash
  • Streamlined protocol with one or 2 additional steps
  • Faster time to results
  • Miniaturizable
  • Wider dynamic range, up to 4 log
  • Longer signal stability

For research use only. Not for use in diagnostic procedures.

 

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100x100_NewestAssays

Make the switch to LANCE TR-FRET

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
OnPoint_2color_100x100
OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components with LANCE donor and acceptor molecules. Or maybe you are you short on time and need an assay developed quickly? Custom LANCE assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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