PerkinElmer

DELFIA TRF Immunoassays

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ELISAs (enzyme-linked immunosorbent assays) have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. While wash-based ELISAs provide excellent quantitation of the target biomolecule, there are several limitations to these assays including a narrow dynamic range and limited signal stability.

 

DELFIA® (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) time-resolved fluorescence technology (TRF) provides a wash-based assay based on a principle and workflow similar to ELISA, but with a wider dynamic range and a stable signal that can be measured from overnight to days, months or years post-assay with proper storage of plates.

In a DELFIA immunoassay, capture antibody is coated to a microplate. Sample is added, followed by a series of wash steps. Following the first series of washes, Europium-labeled detection antibody is added to each well. The plate is washed one final time, and Enhancement Solution is added. The Enhancement Solution releases the Europium from its chelate, allowing the formation of a new, highly fluorescent chelate in solution. Excitation at 320 or 340 nm leads to detectable emission at 615 nm. The amount of analyte is proportional to the emission signal which can be quantitated by interpolation from a standard curve. The time-resolved nature of the detection step minimizes background signal, resulting in a stable, maximized signal-to-background.

DELFIA offers benefits over traditional ELISA including:

  • Wider dynamic range, up to 5 log
  • Greater sensitivity
  • Longer signal stability
  • Miniaturizable
  • Ability to multiplex

 

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100x100_NewestAssays

Make the switch to DELFIA TRF

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can conjugate your antibody or biomolecule to any of our DELFIA TRF chelates. Or maybe you are you short on time and need an assay developed quickly? Custom DELFIA assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your antibody or biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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  • Kinase (5)
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  • Phosphorylation (5)
Antibody Conjugates
  • Anti-6X His (1)
  • Anti-c-Myc (1)
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  • Anti-human IgG (1)
  • Anti-mouse IgG (1)
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  • Anti-phospho-threonine (1)
  • Anti-rabbit IgG (1)
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1-25 of 28 Products & Services

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  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Tb-N1 Streptavidin

    Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
    Price Range : 320.00 USD - 2180.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-HA

    Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
    Price Range : 655.00 USD - 6300.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-GST

    Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
    Price Range : 294.00 USD - 3387.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

    Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
    Price Range : 339.00 USD - 3387.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA®; Eu-N1 Goat anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
    Price Range : 758.00 USD - 3443.00 USD
  • DELFIA Eu-DTPA-DTA Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 rabbit anti-mouse-IgG

    Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.
    Price Range : 290.00 USD - 1256.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Human IgG, 100µg

    DELFIA® Europium-labeled anti-human IgG, for use in DELFIA time-resolved fluorescence (TRF) immunoassays.
    List Price : 355.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Sm-N1 Streptavidin

    Samarium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
    Price Range : 320.00 USD - 2180.00 USD
  • DELFIA Eu-N1-amino Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
    Price Range : 485.00 USD - 4523.00 USD
  • DELFIA Eu-DTPA-ITC Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

    Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
    Price Range : 291.00 USD - 3387.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

    Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
    Price Range : 252.00 USD - 3374.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Anti-phospho-(Ser) 14-3-3 motif, 10µg

    Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for capturing antibodies in DELFIA® TRF kinase assays.
    List Price : 2837.00 USD
  • DELFIA Sm-Labeling kit (Sm-N1-ITC Chelate), 0.2mg

    The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.
    List Price : 564.00 USD
  • DELFIA Eu-N1 ITC chelate & Eu standard, 1 mg

    DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
    List Price : 2229.00 USD
  • DELFIA Eu-DTPA-amino Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-c-myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in DELFIA® TRF assays.
    Price Range : 700.00 USD - 6300.00 USD
  • DELFIA Eu-N1-Iodoacetomido Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
    List Price : 2229.00 USD
  • DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.4 mg

    DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
    List Price : 564.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

    Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
    List Price : 1428.00 USD
  • DELFIA BrdU cell proliferation kit

    DELFIA®Cell proliferation kit, 10 x 96 wells

    DELFIA® time-resolved fluorescence (TRF) cell proliferation kit for measuring cytotoxicity or proliferation in mammalian cells.
    List Price : 743.00 USD
  • DELFIA Tb-DTPA-ITC Chelate and Terbium (Tb) Standard, 1mg

    Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Streptavidin

    DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 397.00 USD
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