PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
OnPoint_2color_100x100
OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
Products & Services (481)
Filters:
Assay Target
  • 4-1BBL (1)
  • 4EBP1 (2)
  • A1AT (1)
  • Adiponectin (1)
  • Akt1/2/3 (4)
  • Albumin (3)
  • ANGPT1 (1)
  • ANGPT3 (1)
  • ANGPTL4 (1)
  • ApoA1 (1)
  • ApoB (1)
  • Aβ1-x (1)
  • Aβ40 (1)
  • Aβ42 (1)
  • B7-H5 (1)
  • BACE-1 (1)
  • BCL-2 (1)
  • BMP-7 (1)
  • BMP-9 (1)
  • BRCA2 (1)
  • BTK (2)
  • BTLA (1)
  • C-peptide (2)
  • CA125 (1)
  • CCL18 (1)
  • CCL2 (1)
  • CCL3 (1)
  • CCL5 (1)
  • CD117 (1)
  • CD276 (1)
  • CD28 (1)
  • CD86 (1)
  • Cofilin (1)
  • Corticosterone (1)
  • Cortisol (1)
  • CREB (2)
  • CRP (1)
  • Cry1Ab/1Ac (1)
  • CXCL1/GROα (1)
  • CXCL10 (2)
  • CYCS (1)
  • Cyr61 (1)
  • E-Cadherin (1)
  • EGF (1)
  • EGFR (4)
  • eIF2α (3)
  • eIF4E (2)
  • EPO (1)
  • ErbB2 (4)
  • ERK1/2 (4)
  • FABP3 (2)
  • Factor VIII (1)
  • FASL (1)
  • FGF23 (1)
  • FLT-1 (1)
  • Frataxin (1)
  • FSH (1)
  • FSP1 (1)
  • G-CSF (1)
  • Galectin 9 (1)
  • GAPDH (2)
  • GDNF (1)
  • Ghrelin (2)
  • GM-CSF (1)
  • Growth Hormone (1)
  • GSK3β (2)
  • HBeAg (1)
  • hCG (1)
  • HCV cAg (1)
  • Histone H2A (1)
  • Histone H3 (5)
  • HLA-E (1)
  • HO-1 (1)
  • HO-2 (1)
  • Hyaluronic Acid (1)
  • ICAM-1 (3)
  • IFN-λ2 (1)
  • IFNα (3)
  • IFNγ (9)
  • IgE (1)
  • IGF1 (2)
  • IGF2 (1)
  • IGFR (2)
  • IgG (3)
  • IgG1 (2)
  • IgG2 (1)
  • IgG2a (1)
  • IgG2b (1)
  • IgG3 (1)
  • IgG4 (1)
  • IgM (3)
  • IKKα (2)
  • IL-1RA (1)
  • IL-23 (1)
  • IL-6 (2)
  • IL10 (4)
  • IL11 (1)
  • IL12 (3)
  • IL12/p70 (1)
  • IL13 (4)
  • IL17 (1)
  • IL17A (6)
  • IL17F (1)
  • IL18 (2)
  • IL18BP (1)
  • IL1α (2)
  • IL1β (7)
  • IL2 (5)
  • IL21 (1)
  • IL22 (2)
  • IL23 (1)
  • IL25 (1)
  • IL28B (1)
  • IL33 (2)
  • IL4 (5)
  • IL5 (2)
  • IL6 (5)
  • IL8 (4)
  • Influenza A NP (1)
  • Insulin (4)
  • IP10 (4)
  • IRAK4 (2)
  • IκBα (1)
  • JNK1/2/3 (2)
  • Kappa light chain (1)
  • LC3B (1)
  • LDH (1)
  • Leptin (2)
  • MANF (1)
  • MBNL1 (1)
  • MEK1 (3)
  • Mesothelin (1)
  • MKK4 (1)
  • MMP-12 (1)
  • MMP-7 (1)
  • MMP1 (1)
  • MMP13 (1)
  • MMP2 (1)
  • MMP23 (1)
  • MMP3 (1)
  • mTOR (2)
  • Myoglobin (1)
  • Neprilysin (1)
  • NFL (2)
  • NFκB (4)
  • NGAL (1)
  • NNMT (1)
  • NT-proBNP (1)
  • Osteoprotegerin (2)
  • OX40L (1)
  • p24 (2)
  • p38 MAPK (2)
  • p53 (2)
  • p62 (1)
  • p70 S6K (4)
  • PCSK9 (1)
  • PD-1 (1)
  • PD-L1 (1)
  • PD-L2 (1)
  • PDGF (1)
  • PEG IgM (1)
  • Periostin (1)
  • Perlecan (1)
  • pNFH (1)
  • PON1 (1)
  • PON2 (1)
  • PSA (1)
  • S100A4 (1)
  • S6RP (2)
  • SCF (1)
  • SHP-1 (2)
  • SHP-2 (4)
  • SHP2 (1)
  • SLP-76 (3)
  • SMAD1 (2)
  • SMAD2 (2)
  • SMAD3 (2)
  • Src (1)
  • STAT1 (4)
  • STAT3 (3)
  • STAT4 (1)
  • STAT5 (3)
  • STAT6 (1)
  • SYK (3)
  • TDP-43 (1)
  • Testosterone (1)
  • TFF3 (1)
  • TGF-β1 (2)
  • TIMP2 (1)
  • TNFα (9)
  • Transthyretin (1)
  • VCAM-1 (1)
  • VEGF-A (1)
  • VEGF2 Receptor (1)
  • VEGFR1 (1)
  • VEGFR2 (2)
  • Vimentin (1)
  • VISTA (1)
  • Zap70 (1)
  • β-NGF (1)
Target Species
  • Human (3)
  • Mouse (1)
Application
  • Antibody binding (3)
  • Epigenetics (6)
  • High biotin samples (7)
  • Isotyping (8)
  • Kinase (74)
  • Multiplexing (54)
  • Protein Analysis & Detection (386)
  • Protein-nucleic acid interactions (4)
  • Protein-protein interactions (60)
Assay Target Class
  • Acetylated protein (1)
  • Aminoglycan (1)
  • Antibody (16)
  • Collagen (1)
  • Cytokine (113)
  • DNA (1)
  • Histone (6)
  • Hormone (16)
  • Peptide (5)
  • Phosphoprotein (74)
  • Protein (159)
Therapeutic Area
  • Agriculture/Plant (3)
  • Angiogenesis (8)
  • Autophagy (2)
  • Biologics/Bioprocess (22)
  • Cardiovascular (27)
  • Central Nervous System (36)
  • Epigenetics (6)
  • Immuno-oncology (2)
  • Inflammation (103)
  • Kidney (1)
  • Metabolic (57)
  • NASH (6)
  • Neuroinflammation (5)
  • Oncology (61)
  • Virology (7)
Product Brand Name
  • AlphaLISA (336)
  • AlphaLISA SureFire Ultra (50)
  • AlphaLISA SureFire Ultra Multiplex (43)
  • AlphaPlex (33)
  • AlphaScreen (17)
  • AlphaScreen SureFire (2)
Detection Method
  • Alpha (459)
Molecular Modification
  • Acetylation (1)
  • Methylation (3)
  • Phosphorylation (4)
  • Total (1)
  • Unmodified (1)

Sort by

1-25 of 481 Products & Services

  • FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 542.00 USD - 11900.00 USD
  • BCL-2 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
    Price Range : 781.00 USD - 25400.00 USD
  • IL-8 (human) AlphaLISA Detection Kit

    The AlphaLISA Human IL8 Detection Kit is designed for the quantitative determination of human IL-8 in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • IL-1β (mouse) AlphaLISA Detection Kit

    The AlphaLISA® Mouse Interleukin-1ß (IL-1ß) Detection Kit is designed for detection and quantitation of mouse IL-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • Cardiac Troponin I (human) AlphaLISA Detection Kit

    The AlphaLISA® Human cardiac Troponin I Detection Kit is designed for detection and quantitation of human cardiac Troponin I in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3652.00 USD - 28500.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead

    IL-10 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of human IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • IL-8 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1334.00 USD - 12900.00 USD
  • AlphaLISA Acceptor Bead Antibody Coated

    Anti-goat IgG (Fc specific) AlphaLISA® Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to anti-goat IgG antibody. These beads can be used to capture goat IgG antibodies. The antibody is Fc-specific, and is a rabbit polyclonal F(ab')2 fragment.
    Price Range : 736.00 USD - 30200.00 USD
  • SCF (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 542.00 USD - 11900.00 USD
  • CD86 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD86 enables the quantitative determination of human CD86 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 752.00 USD - 18100.00 USD
  • CCL2/MCP1 (mouse/rat) AlphaLISA Detection Kit

    The AlphaLISA® Mouse/Rat C-C Motif Chemokine 2/Monocyte Chemoattractant Protein 1 (CCL2/MCP1) Detection Kit is designed for detection and quantitation of mouse or rat CCL2/MCP1 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Mouse CCL2/MCP1 can be quantitatively measured in mouse serum. The analyte in this kit is mouse CCL2/MCP1.
    Price Range : 3651.00 USD - 28500.00 USD
  • BTLA (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human BTLA enables the quantitative determination of human B- and T-lymphocyte attenuator (BTLA, CD272) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • IL-8 (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA Human IL8 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-8 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 871.00 USD - 12600.00 USD
  • AlphaLISA SureFire Ultra Lysate

    AlphaLISA SureFire Ultra Phospho-SHP-2 (Tyr542) Control Lysate

    Extra control lysate for the AlphaLISA SureFire Ultra phospho-SHP-2 (Tyr542) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-SHP-2 assay kit, but can also be ordered separately.
    List Price : 24.00 USD
  • Total NFκB AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-NFκB assay.
    Price Range : 553.00 USD - 36700.00 USD
  • IgM (human) AlphaLISA Detection Kit

    The AlphaLISA® Human IgM Protein Detection Kit is designed for detection and quantitation of human IgM in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 952.00 USD - 7900.00 USD
  • NT-proBNP (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human NT-proBNP enables the quantitative determination of human N-terminal prohormone of brain natriuretic peptide (NT-proBNP) in buffer, serum, or plasma using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 13300.00 USD
  • AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

    The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
    Price Range : 553.00 USD - 35300.00 USD
  • NFL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human neurofilament light chain protein (NFL) enables the quantitative determination of human FABP3 in buffer and cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 939.00 USD - 21000.00 USD
  • ICAM-1 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Intercellular Adhesion Molecule-1 (ICAM-1) Detection Kit is designed for detection and quantitation of human ICAM-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies target the extracellular domain of ICAM-1. The analyte in this kit consists of the extracellular domain of human ICAM-1.
    Price Range : 3652.00 USD - 28500.00 USD
  • Total MEK1 AlphaLISA SureFire Ultra Assay Kit

    The AlphaLISA® SureFire® Ultra™ Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
    Price Range : 553.00 USD - 35300.00 USD
  • Alpha Terbium SureFire Ultra Multiplex TBSU

    Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
    Price Range : 416.00 USD - 27600.00 USD
  • AlphaLISA SureFire Ultra Total GAPDH Assay Kit

    The AlphaLISA® SureFire® Ultra™ Total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for cell number.
    Price Range : 553.00 USD - 35300.00 USD
  • Myoglobin (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Myoglobin Detection Kit is designed for detection and quantitation of human myoglobin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3652.00 USD - 28500.00 USD
  • IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

    The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 752.00 USD - 22500.00 USD
  • Jump to:
Resource Library (558)