PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

AlphaLISA Homogenous Immunoassays
AlphaLISA Homogenous Immunoassays

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 512 Products & Services

  • AlphaLisa Analyte 2

    NFL (human), lyophilized (AlphaLISA)

    Recombinant human neurofilament light chain protein (NFL) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human NFL detection kit, but can also be ordered separately.
    List Price : 867.00 USD
  • AlphaLISA immunoassay kits

    NFL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human neurofilament light chain protein (NFL) enables the quantitative determination of human FABP3 in buffer and cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 20000.00 USD
  • AlphaLISA immunoassay kits

    CD276 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD276 enables the quantitative determination of human Cluster of Differentiation 276 (CD276, B7-H3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total NFκB Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-NFκB p65 (Ser536) + Total NFκB p65 assay kit is used to measure both the phosphorylation (on Ser536) and total levels of endogenous NFκB in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure NFκB phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaScreen Acceptor Beads

    AlphaScreen Biotinylated-GST

    Biotinylated-GST, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA®, AlphaScreen®, or AlphaPlex™) no-wash assays.
    List Price : 599.00 USD
  • AlphaLISA immunoassay kits

    4-1BBL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human 4-1BBL enables the quantitative determination of 4-1BB ligand in buffer, cell culture media, cell supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLisa Analyte 2

    Cytochrome C (human), lyophilized (AlphaLISA)

    Recombinant human cytochrome C (CYCS) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CYCS detection kit, but can also be ordered separately.
    List Price : 867.00 USD
  • AlphaLISA immunoassay kits

    IL-12 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine IL-12 enables the quantitative determination of porcine interleukin-12 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 715.00 USD - 21400.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-STAT3 (Tyr705) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT3 (Tyr705) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total MEK1 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-MEK1 (Ser218/222) + Total MEK1 assay kit is used to measure both the phosphorylation (on Ser218/222) and total levels of endogenous MEK1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure MEK1 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaScreen GST Detection Kit

    AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
    Price Range : 214.00 USD - 16400.00 USD
  • AlphaLISA immunoassay kits

    HLA-C (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaLISA immunoassay kits

    AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

    The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of human Programmed Cell Death Protein 1 (PD-1) in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 769.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    CCL18 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLISA SureFire Ultra kits

    Total p70 S6K AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-p70 S6K assay.
    Price Range : 526.00 USD - 34900.00 USD
  • AlphaLISA immunoassay kits

    Albumin (human) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for detection of human serum albumin (HSA) in urine, buffered solution, or cell culture medium.
    Price Range : 3470.00 USD - 27100.00 USD
  • AlphaLISA immunoassay kits

    Insulin-like Growth Factor 1 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Insulin-like Growth Factor 1 (IGF1) Detection Kit is designed for detection and quantitation of human IGF1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-STAT5 (Tyr694/699) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT5 (Tyr694/699) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA assay principle

    IL-23 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human IL-23 Detection Kit is designed for detection and quantitation of human interleukin 23 in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 736.00 USD - 22100.00 USD
  • AlphaScreen FLAG (M2) Detection Kit

    AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 
    Price Range : 214.00 USD - 16400.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total STAT3 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-STAT3 (Tyr705) + Total STAT3 assay kit is used to measure both the phosphorylation (on Tyr705) and total levels of endogenous STAT3 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT3 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaLISA assay principle

    TSH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human TSH enables the quantitative determination of human thyroid stimulating hormone (TSH) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 736.00 USD - 22100.00 USD
  • AlphaScreen Phosphotyrosine (PY20) Assay Kit

    AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
    Price Range : 214.00 USD - 16400.00 USD
  • AlphaLISA immunoassay kits

    IL-1β (bovine) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 768.00 USD - 12300.00 USD
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