PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

AlphaLISA_general320x320
AlphaLISA_general320x320

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
OnPoint_2color_100x100
OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 634 Products & Services

  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

    The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 29200.00 USD
  • Alpha donor antibody coated

    Anti-HRP Alpha Donor Beads

    Alpha Donor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for Alpha no-wash detection, binding, and other assays.
    Price Range : 487.00 USD - 8800.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire® Ultra™ total ErbB2 Assay Kit

    The AlphaLISA® SureFire® Ultra™ total ErbB2 assay kit can be used to measure levels of ErbB2 (HER2) (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.
    Price Range : 504.00 USD - 29200.00 USD
  • AlphaLISA immunoassay kits

    IL-7 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin 7 (IL7) Detection Kit is designed for detection and quantitation of human IL-7 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • pNFH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human phosphorylated neurofilament heavy chain protein (pNFH) enables the quantitative determination of human pNFH in plasma or cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 750.00 USD - 11100.00 USD
  • Alpha donor antibody coated

    Anti-Rabbit IgG Alpha Donor beads

    Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
    Price Range : 502.00 USD - 9100.00 USD
  • AlphaLISA immunoassay kits

    AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

    The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of human Programmed Cell Death Protein 1 (PD-1) in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    IL-6 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 6 (IL-6) enables the quantitative determination of porcine IL-6 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Total Cofilin Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two targets in each well of an assay plate. This kit can be used in combination with the AlphaLISA SureFire Ultra phospho-cofilin assay to normalize to levels of total cofilin.
    Price Range : 378.00 USD - 22800.00 USD
  • SureFire principle

    AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

    The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • PDGF (BB dimer) (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • SureFire principle

    AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

    The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLISA immunoassay kits

    MMP-2 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Matrix Metalloproteinase-2 (MMP2) Detection Kit is designed for detection and quantitation of human MMP2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP2 and pro-MMP2.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    MMP-12 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.
    Price Range : 465.00 USD - 10200.00 USD
  • SureFire principle

    AlphaScreen SureFire Histone H3 (p-Ser10) Assay Kit

    The AlphaScreen® SureFire® p-Histone H3 (Ser10) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (phosphorylated on Ser10) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • IgA (bovine) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of bovine IgA in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 672.00 USD - 10700.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex™ 545 IL-6 Detection Kit 

    The AlphaPlex™ 545 (Terbium) IL-6 Detection Kit is designed for the quantitative determination of human IL6 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
    Price Range : 633.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    EGF (human) AlphaLISA Detection Kit

    The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    CHO-P AlphaLISA Detection Kit

    AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
    Price Range : 792.00 USD - 6600.00 USD
  • AlphaLISA immunoassay kits

    Cry1F AlphaLISA Detection Kit

    The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 625.00 USD - 9700.00 USD
  • Anti-IgG1 AlphaLISA Acceptor beads

    AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
    Price Range : 594.00 USD - 24400.00 USD
  • BMP-9 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human BMP-9 enables the quantitative determination of human Bone Morphogenetic Protein 9 (BMP-9) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 18800.00 USD
  • AlphaLISA SureFire Ultra kits

    Phospho-VEGFR2 (Tyr951) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ phospho-VEGFR2 assay is a sandwich immunoassay for quantitative detection of VEGFR (phosphorylated on Tyr951) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 30400.00 USD
  • AlphaLISA immunoassay kits

    HEK293 HCP AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for HEK293 host cell proteins (HCP) enables the quantitative determination of contaminating HEK293 host cell proteins in buffer, culture media, lysis buffer, and cell lysates for bioprocess applications using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1259.00 USD - 10200.00 USD
  • IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit

    The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 1539.00 USD - 10200.00 USD
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