PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 480 Products & Services

  • C-Reactive Protein (human) AlphaLISA Detection Kit

    The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • p62 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for p62 enables the quantitative determination of p62 in human cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 901.00 USD - 12700.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-Rabbit IgG Alpha Donor beads

    Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
    Price Range : 603.00 USD - 10900.00 USD
  • TIM-3 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 752.00 USD - 18100.00 USD
  • IL-18BP (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human IL-18BP enables the quantitative determination of human interleukin 18-binding protein in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 939.00 USD - 14000.00 USD
  • NT-proBNP (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human NT-proBNP enables the quantitative determination of human N-terminal prohormone of brain natriuretic peptide (NT-proBNP) in buffer, serum, or plasma using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 13300.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-Mouse IgG Alpha Donor beads

    Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
    Price Range : 603.00 USD - 10900.00 USD
  • HVEM (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human herpesvirus entry mediator (HVEM) enables the quantitative determination of human HVEM (also known as TNFRSF14, CD270) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • GAD67 (Human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
    Price Range : 968.00 USD - 30900.00 USD
  • MMP-23 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human matrix metalloproteinase 23 (MMP-23) enables the quantitative determination of human MMP-23 in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 901.00 USD - 13300.00 USD
  • PCSK9 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) Detection Kit is designed for detection and quantitation of human PCSK9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. One antibody targets the pro-domain of PCSK9.
    Price Range : 1905.00 USD - 12600.00 USD
  • CXCL4/PF4 (Human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
    Price Range : 775.00 USD - 23300.00 USD
  • Receptor Tyrosine-Protein Kinase ERbB-2 (HER2) AlphaLISA Detection Kit

    The AlphaLISA® Human epidermal growth factor receptor 2 (ErbB-2, NEU, or HER2) Detection Kit is designed for detection and quantitation of human ErbB-2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 12600.00 USD
  • AlphaLISA Acceptor Bead Antibody Coated

    Anti-human IgG (Fc specific) AlphaLISA Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to anti-human IgG antibody. These beads can be used to capture human IgG antibodies. The antibody conjugated to these beads is Fc-specific, and is a mouse polyclonal antibody.
    Price Range : 736.00 USD - 30200.00 USD
  • Alpha Terbium SureFire Ultra Multiplex TBSU

    Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
    Price Range : 416.00 USD - 27600.00 USD
  • MMP-2 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Matrix Metalloproteinase-2 (MMP2) Detection Kit is designed for detection and quantitation of human MMP2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP2 and pro-MMP2.
    Price Range : 1905.00 USD - 12600.00 USD
  • CYR61 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 912.00 USD - 17600.00 USD
  • Streptavidin AlphaLISA® Acceptor beads

    AlphaLISA® Acceptor beads conjugated to streptavidin. These beads can be used to capture biotinylated proteins, peptides, nucleic acids (DNA and RNA), and small molecules for AlphaLISA no-wash assays.
    Price Range : 736.00 USD - 30200.00 USD
  • PON-1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 912.00 USD - 17600.00 USD
  • IL-4 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin 4 (IL4) Detection Kit is designed for detection and quantitation of human IL4 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1905.00 USD - 13300.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-DNP Alpha Donor Beads

    Alpha Donor beads conjugated to anti-DNP antibody. These beads can be used to capture DNP-labeled proteins, peptides, and small molecules.
    Price Range : 1995.00 USD - 23300.00 USD
  • LAG-3 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 940.00 USD - 18100.00 USD
  • Cry1F AlphaLISA Detection Kit

    The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 752.00 USD - 11700.00 USD
  • PerkinElmer

    SureFire Ultra MultiPlex Tb CaptSure Acceptor Beads

    CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
    Price Range : 316.00 USD - 12900.00 USD
  • CCL1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CCL1 enables the quantitative determination of human chemokine (C-C motif) ligand 1 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 901.00 USD - 12700.00 USD
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