PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

AlphaLISA Homogenous Immunoassays
AlphaLISA Homogenous Immunoassays

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
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OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 558 Products & Services

  • Assay principle of Alpha Terbium SureFire Ultra assay

    Total Cofilin Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two targets in each well of an assay plate. This kit can be used in combination with the AlphaLISA SureFire Ultra phospho-cofilin assay to normalize to levels of total cofilin.
    Price Range : 395.00 USD - 26200.00 USD
  • Heme oxygenase 2 AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • AlphaScreen GST Detection Kit

    AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
    Price Range : 214.00 USD - 16400.00 USD
  • AlphaLISA immunoassay kits

    Cortisol (human) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.
    Price Range : 829.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    CD276 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD276 enables the quantitative determination of human Cluster of Differentiation 276 (CD276, B7-H3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaScreen Phosphotyrosine (PT66) Assay Kit

    AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
    Price Range : 271.00 USD - 16400.00 USD
  • N-cadherin (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human N-cadherin enables the detection and quantitation of human N-Cadherin in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 547.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    COL1A1 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human COL1A1 Detection Kit is designed for detection and quantitation of human COL1A1 in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 531.00 USD - 11600.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • CCL18 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

    The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
    Price Range : 526.00 USD - 33600.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

    The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1788.00 USD - 19100.00 USD
  • SureFire principle

    AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

    The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

    AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
    Price Range : 214.00 USD - 16400.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • Host Cell Residual DNA AlphaLISA Detection Kit

    The AlphaLISA® Host Cell Residual DNA Detection Kit is designed for detection and quantitation of DNA contaminants in cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay. This kit can be used with DNA from different prokaryotic and eukaryotic species.
    Price Range : 828.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    Cry1F AlphaLISA Detection Kit

    The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 715.00 USD - 11100.00 USD
  • IL-8 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1268.00 USD - 12300.00 USD
  • AlphaLISA immunoassay kits

    p24 (human HIV) AlphaLISA Detection Kit

    The AlphaLISA® Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Detection Kit is designed for detection and quantitation of HIV p24 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • AlphaLISA Cellular Epigenetics kits

    AlphaLISA di/mono-methylated-Histone H3 Lysine 27 (H3K27me2-1) Cellular Detection Kit

    This kit is designed for the detection of di/mono-methylated Histone H3 Lysine 27 (H3K27me2-1) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 828.00 USD - 14800.00 USD
  • PAI-1 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Plasminogen Activator Inhibitor-1 (PAI-1) Detection Kit is designed for detection and quantitation of human PAI-1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the active glycosylated human PAI-1.
    Price Range : 3470.00 USD - 27100.00 USD
  • AlphaLISA immunoassay kits

    Influenza A Nucleoprotein (NP) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for influenza A nucleoprotein (NP) enables the quantitative determination of nucleoprotein in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • SureFire principle

    AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

    The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1788.00 USD - 19100.00 USD
  • AlphaLISA immunoassay kits

    CYR61 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 867.00 USD - 16700.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-BAD (Ser112) Kit

    The AlphaScreen® SureFire® p-BAD (Ser112) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser112) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
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