PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

AlphaLISA_general320x320
AlphaLISA_general320x320

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
OnPoint_2color_100x100
OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 627 Products & Services

  • SureFire principle

    AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

    The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaLISA immunoassay kits

    IL-4 (bovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 531.00 USD - 11600.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-AKT (Ser473) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-AKT (Ser473) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA immunoassay kits

    IL-22 (human/rat) AlphaLISA Detection kit

    The AlphaLISA® Human/rat IL-22 Detection Kit is designed for detection and quantitation of human or rat interleukin 22 in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 828.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    AST (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 867.00 USD - 16700.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • MMP13 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human MMP-13 Detection Kit is designed for detection and quantitation of human MMP-13 in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 769.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    LDHA (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human lactate dehydrogenase subunit A (LDHA) enables the quantitative determination of human LDHA in cell lysates and serum using a homogeneous AlphaLISA assay (no wash steps). Please note this is NOT a cell cytotoxicity assay, as the assay does not measure secreted LDH.
    Price Range : 867.00 USD - 16700.00 USD
  • SureFire principle

    AlphaScreen SureFire p53 (p-Ser15) Assay Kit

    The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • alphascreen surefire total

    Total ERK (monoclonal) AlphaScreen SureFire Assay Kit

    The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1788.00 USD - 19100.00 USD
  • TDP-43 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human TDP-43 is designed for the quantitative determination of Transactive response DNA binding protein 43 (TDP43) in human serum, cerebrospinal fluid (CSF), cell lysates, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 742.00 USD - 24100.00 USD
  • TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 768.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    Adiponectin (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA SureFire Ultra kits

    Phospho-ZAP-70 (Tyr319) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ phospho-ZAP-70 assay is a sandwich immunoassay for quantitative detection of ZAP-70 (phosphorylated on Tyr319) in cellular lysates using Alpha Technology.
    Price Range : 526.00 USD - 34900.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

    The AlphaScreen® SureFire® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • SureFire principle

    AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

    The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex™ 545 Human Insulin Detection Kit

    The AlphaPlex™ 545 (Terbium) Human Insulin Detection Kit is designed for the quantitative determination of human Insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
    Price Range : 549.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    CHO-P AlphaLISA Detection Kit

    AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
    Price Range : 905.00 USD - 7500.00 USD
  • AlphaLISA immunoassay kits

    IL-10 (bovine/ovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for bovine IL-10 enables the detection and quantitation of bovine IL-10 (bIL10) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit also detects Ovine IL-10.
    Price Range : 828.00 USD - 14900.00 USD
  • AlphaLISA immunoassay kits

    CD117 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD117 enables the quantitative determination of human CD117 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • GDNF (human) AlphaLISA Detection kit

    The AlphaLISA® Human GDNF Detection Kit is designed for detection and quantitation of human GDNF in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 769.00 USD - 12000.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit

    The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaLISA SureFire Ultra kits

    Total AKT1/2/3 AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total Akt1/2/3 assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Akt1/2/3 assay.
    Price Range : 526.00 USD - 34900.00 USD
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