PerkinElmer

AlphaLISA Immunoassays

OF

ELISA, western blot, and other immunodetection technologies have commonly been used for quantitation of biomarkers and other analytes in a variety of sample types. These methods necessitate long, tedious protocols and often provide limited detectable range.

AlphaLISA® provides a homogenous (no-wash) alternative to ELISAs, with a simple, streamlined workflow that can be used to detect and quantitate biomolecules in both simple and complex sample types. AlphaLISA is a bead-based luminescent amplification assay, offering greater sensitivity, a wider dynamic range and small sample sizes over traditional ELISA.

 

In the AlphaLISA assay, a biotinylated antibody and an antibody-conjugated AlphaLISA Acceptor bead are used to capture the target analyte. The biotinylated antibody associates with an Alpha streptavidin-coated Donor bead. When the analyte is present in the sample, the Donor and Acceptor beads are brought together. Upon excitation, a photosensitizer inside the Donor bead converts ambient oxygen to an excited singlet state. Singlet oxygen diffuses up to 200 nm to produce a chemiluminescent reaction in the Acceptor bead, leading to light emission. The amount of light is proportional to the amount of analyte present in your sample.

Alpha offers the benefits over ELISAs including:

  • No-wash
  • Faster time to results
  • Requires a fraction of the sample size, ~5µL
  • Wider dynamic range up to 5 log
  • Improved sensitivity
  • Ability to multiplex
  • Ability to study large protein complexes

For research use only. Not for use in diagnostic procedures.

 

AlphaLISA Homogenous Immunoassays
AlphaLISA Homogenous Immunoassays

Make the switch to AlphaLISA

Switching to a new immunoassay platform may seem like a large undertaking, but it doesn’t have to be. We have a team of experts ready to help you make the switch. We have a wide range of support options from development guides to or Application Support Knowledgebase (ASK) and from technical support, to our global Field Application Scientist who are hands-on subject matters in assay development.
OnPoint_2color_100x100
OnPoint_2color_100x100

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can custom conjugate your antibody or biomolecule to any bead, or biotinylate or DIG-label your targets. Or maybe you are you short on time and need an assay developed quickly? Custom Alpha assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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1-25 of 482 Products & Services

  • ANGPTL4 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • Frataxin (human) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for detection and quantitation of human Frataxin (hFXN) in cell and tissue lysates.
    Price Range : 828.00 USD - 12000.00 USD
  • AlphaLisa Analyte 2

    NFL (human), lyophilized (AlphaLISA)

    Recombinant human neurofilament light chain protein (NFL) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human NFL detection kit, but can also be ordered separately.
    List Price : 867.00 USD
  • 4-1BBL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human 4-1BBL enables the quantitative determination of 4-1BB ligand in buffer, cell culture media, cell supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • Testosterone AlphaLISA Detection Kit

    The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 768.00 USD - 12000.00 USD
  • IL-17A (human) AlphaLISA Biotin-Free Detection Kit

    AlphaLISA Human IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of human IL-17A in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 828.00 USD - 12000.00 USD
  • NFL (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human neurofilament light chain protein (NFL) enables the quantitative determination of human FABP3 in buffer and cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 20000.00 USD
  • IL-17A (bovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 830.00 USD - 23300.00 USD
  • Residual Protein A AlphaLISA Detection Kit

    The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 905.00 USD - 7500.00 USD
  • PerkinElmer

    SureFire Ultra MultiPlex Tb CaptSure Acceptor Beads

    CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
    Price Range : 300.00 USD - 12300.00 USD
  • TNFα (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 828.00 USD - 12000.00 USD
  • CD117 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD117 enables the quantitative determination of human CD117 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • Albumin (human) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for detection of human serum albumin (HSA) in urine, buffered solution, or cell culture medium.
    Price Range : 3470.00 USD - 27100.00 USD
  • BACE-1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • CCL5/RANTES (human) AlphaLISA Detection Kit

    The AlphaLISA® Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) Detection Kit is designed for detection and quantitation of human CCL5/RANTES in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3470.00 USD - 27100.00 USD
  • Total SMAD2 AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total SMAD2 assay is a sandwich immunoassay for quantitative detection of total SMAD2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-SMAD2.
    Price Range : 526.00 USD - 34900.00 USD
  • Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.
    Price Range : 678.00 USD - 27900.00 USD
  • AlphaPlex Acceptor Bead 545 Antibody

    Anti-rabbit IgG AlphaPlex-545 (Tb) Acceptor Beads

    AlphaPlex™-545 (Terbium) Acceptor beads conjugated to anti-rabbit IgG antibody, for use in Alpha no-wash multiplexing assays.
    Price Range : 700.00 USD - 28700.00 USD
  • CCL2/MCP1 (mouse/rat) AlphaLISA Detection Kit

    The AlphaLISA® Mouse/Rat C-C Motif Chemokine 2/Monocyte Chemoattractant Protein 1 (CCL2/MCP1) Detection Kit is designed for detection and quantitation of mouse or rat CCL2/MCP1 in buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Mouse CCL2/MCP1 can be quantitatively measured in mouse serum. The analyte in this kit is mouse CCL2/MCP1.
    Price Range : 3470.00 USD - 27100.00 USD
  • IL-4 (rhesus) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for rhesus interleukin-4 (IL-4) enables the quantitative determination of rhesus IL-4 in buffered solution, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • AlphaLISA Acceptor Bead Antibody Coated

    Anti-DIG AlphaLISA Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.
    Price Range : 700.00 USD - 28700.00 USD
  • EPO (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Erythropoietin (EPO) Detection Kit is designed for detection and quantitation of human EPO in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3470.00 USD - 27100.00 USD
  • Alpha Donor Bead Antibody Conjugated

    Anti-HRP Alpha Donor Beads

    Alpha Donor beads conjugated to anti-HRP antibody. These beads can be used to capture HRP-fusion proteins for Alpha no-wash detection, binding, and other assays.
    Price Range : 556.00 USD - 10000.00 USD
  • Alpha Terbium SureFire Ultra Multiplex TBSU

    Phospho-SMAD3 (Ser423/425) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD3 (Ser423/425) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

    The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
    Price Range : 526.00 USD - 33600.00 USD
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