PerkinElmer

Immunoassays

Traditional enzyme-linked immunosorbent assays (ELISAs) are often used to detect and quantify the levels of an analyte from a range of complex sample matrices (e.g. human serum, urine, or blood) where an abundance of other proteins and molecules are present. While compatible with complex matrices, a colorimetric ELISA is susceptible to the constraint of a narrow linear range for the optical density (OD), which is common to absorbance-based measurements. The smaller dynamic range (~ 2 logs) of the assay decreases the likelihood that your unknown sample concentration will fall within the standard curve and introduces the challenge of testing multiple dilutions from the same, potentially limited, sample. Repeat dilution testing of the same sample also requires additional assay wells and often leads to purchasing additional kits or coating additional assay plates to accurately quantify all your samples. Chemiluminescent ELISAs can be more sensitive, but require vast amounts of antibody, increasing the cost of the assay, and lack signal stability due to the nature of the rapid decline in luminescence over time.

Similarly, western blotting is widely used as a semi-quantitative method to determine and compare amounts of protein. These assays can suffer from limited sensitivity and throughput. Because western blotting involves a multistep protocol, variation and errors can occur at any step, reducing the reliability and reproducibility of results.

PerkinElmer offers a range of immunoassay technologies that enable selection of the optimal immunoassay platform for your requirements. From sample compatibility to sample volume, throughput, detectable range, signal window, signal stability, reproducibility, automation compatibility, and desired speed of results, PerkinElmer offers immunoassay technology platforms, pre-configured kits, and customizable reagents for superior results.

DELFIA TRF

DELFIA TRF

DELFIA® TRF is a proven, robust wash-based immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improved assay dynamic range. DELFIA also offers the ability for multiplexing. DELFIA TRF is compatible with the widest range of sample types, ranging from simple buffers, cell supernatants, and cell lysates to complex biological samples, including tissue, serum, plasma, and blood.

Below is a workflow comparison between a DELFIA TRF assay and a traditional ELISA.

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LANCE TR-FRET

LANCE TR-FRET

LANCE® TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with fastest results and no wash steps. LANCE TR-FRET is compatible with buffer, cellular supernatant and cell lysate samples.

Below is a workflow comparison between a LANCE TR-FRET assay and a traditional ELISA.

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Alpha

Alpha Technology

Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility. Alpha technology is compatible with a wide range of sample types ranging from simple buffers, cell supernatants, and cell lysates to complex tissue, serum, plasma, and other biological samples.

Alpha offers the benefits over ELISAs including:

  • Faster time to results
  • Ability to quantitate large protein complexes, nucleosomes, and viral particles
  • Option for multiplexing

Below is a workflow comparison between an Alpha assay and a traditional ELISA.

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Explore Assays by Therapeutic Areas

Immuno-oncology Reagents

Immuno-oncology (I-O) is a rapidly expanding area of personalized medicine. ...

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Inflammation

Inflammation is a natural marker for bodily health, and abnormal inflammato ...

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Biotherapeutics Research

Biotherapeutics allow researchers to explore new mechanisms for the treatme ...

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Explore Assays by Target Area

Biomarker Detection Reagents

Our biomarker detection reagents and kits are designed to excel in applicat ...

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Protein Kinase Research Reagents

Cancer, diabetes, and Alzheimer’s research can benefit from the study of ph ...

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Epigenetic Research Reagents

As the understanding of the role of epigenetic changes in various disease s ...

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GPCR Research Reagents

GPCR research remains a widely studied area in drug discovery. As the compl ...

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Protein Kinase Research

Protein kinases are key focus targets in drug discovery. Kinases regulate m ...

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Protein-Protein Interaction Research Reagents

The interactions and binding of proteins are implicated in a large number o ...

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Featured Products

Microplates

A microplate is a critical part of your discovery process. Microplates have ...

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EnVision 2105 Multimode Plate Reader

The EnVision® 2105 multimode plate reader provides exceptional speed, ultra ...

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EnSight Multimode Plate Reader

The EnSight™ multimode plate reader offers HTS Alpha, Ultra-sensitive Lumin ...

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VICTOR Nivo Multimode Microplate Reader

Now with Alpha Technology, the VICTOR® Nivo™ system packs all popular detec ...

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Find the immunoassay platform that is right for you