Is a homogeneous, luminescence proximity assay technology. When Alpha Donor and Acceptor beads are brought together, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are coated with various biomolecules and enable detection of unique biological events. From biomarker detection to inhibitor screening and identification, there’s an AlphaLISA® assay to suit your needs. Plus, Alpha SureFire® Ultra™ provides no-wash cellular kinase assays for the detection of phosphorylated proteins, allowing you to look at downstream cell signaling events.
Is a homogeneous proximity assay technology that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. We offer a selection of LANCE® Ultra TR-FRET biomarker immuno-oncology detection kits as well as an array of kits for cell signaling.
Assays are widely used assays not only in immuno-oncology studies, but in the drug discovery process in general. They allow us to monitor cell health giving us key insights on the effectiveness of potential drugs. We offer a range of cytotoxicity and cell proliferation assays, including cell proliferation via DNA synthesis or ATP monitoring. Cell-mediated cytotoxicity, including ADCC and CAR-T cell-mediated lysis, with DELFIA TRF cell-mediated cytotoxicity and CR-51 assays.
Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can label your assay components for use in any of our assay technologies – Alpha, LANCE and DELFIA. Or maybe you are you short on time and need an assay developed quickly? Custom assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.
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The AlphaLISA® SureFire® Ultra™ total JNK1/2 assay is a sandwich immunoassay for quantitative detection of JNK1/2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
The AlphaLISA® mouse Interferon β (IFNβ) Detection Kit is designed for detection and quantitation of mouse IFNβ in buffered solution, or cell culture in a homogeneous AlphaLISA assay (no wash steps).
The AlphaLISA® cyno PD-1/PD-L1 binding kit is designed for the detection of binding activity between cyno PD-1 and PD-L1, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
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