High Content Screening & Analysis

High-Content Analysis (HCA) or High-Content Screening (HCS) combines high-throughput automated imaging and analysis to extract quantitative multi-parametric data at the single-cell level. Originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, today high-content screening is established in a far broader area of the life science space as an unbiased imaging method to assess cellular function.

High-Content Imaging (HCI) is typically defined as lower throughput automated microscopy (<100,000 samples or data points) compared to high-content screening. However, high-content imaging is often used interchangeable with the terms high-content screening and high-content analysis.1

High-content screening offers a number of advantages over traditional techniques:

  • More information from cellular samples
    Fully automated imaging and unbiased quantitative image analysis exploits the full potential of microscopy, improving your lab’s throughput and productivity.
  • More meaningful results
    Screening can be performed in more physiologically and biologically relevant systems than in classical biochemical assays.
  • More questions answered simultaneously
    Data sets are more information-rich compared to non-imaging read-outs, allowing a number of questions to be answered simultaneously.


  • Buchser, William, et al. "Assay development guidelines for image-based high content screening, high content analysis and high content imaging." Assay guidance manual [Internet] (2014).


PerkinElmer provides a range of solutions - including instruments, informatics, automation and microplates - for high content imaging and analysis, and phenotypic screening of live cells, 3D cell models, stem cells, that can be tailored by our team of experts to meet your lab’s needs.

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See how the technologies behind our market-leading products help you succeed with even the most challenging applications and assays.

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Research Areas

Discover more about how PerkinElmer high-content analysis products can advance your research in areas such as cancer, neuroscience, infectious disease, toxicity and stem cells.

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Discover how PerkinElmer high-content screening solutions can benefit your research in both new and established applications.

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PerkinElmer offers a range of solutions to enable you to develop your assays with ease. Find out more about the assays you are interested in and how our products can help.

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Meet our Users

Read how scientists around the globe are using our high-content screening products and services to advance their research in a variety of fields, including rare genetic disease, infectious disease, cancer, and RNAi screening.

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