PerkinElmer

GrowDex Hydrogels

How to use Growdex hydrogels

We offer a range of products for scalable animal-free 3D cell culture applications, providing convenience and consistent results. GrowDex® hydrogels are natural nanofibers of cellulose extracted from wood and sourced from sustainable and responsibly managed forests. Supplied as ready-to-use, prepacked syringes, GrowDex mimics the extracellular matrix and supports cell growth and differentiation. These animal-free matrices are a proven solution for automation and high throughput screening. They can be used for 3D cell culture for spheroids and organoids, in personalised medicine, regenerative medicine, organ-on-a-chip models, drug release studies and more.

Cells can be recovered from GrowDex for downstream processing, with a one-step enzymatic process using our GrowDase.

Benefits of GrowDex hydrogels:

  • Animal-free: No animal biomolecules interfering with readouts.
  • Reproducible: Comprising nanofibrillar cellulose and purified water only, GrowDex hydrogels are manufactured to the highest standards with strict quality control criteria to ensure lot-to-lot reproducibility.
  • Reliable: Robust and sustained performance regardless of the production lot ordered.
  • Stable: Used and stored at room temperature, GrowDex hydrogels can be used in liquid handling systems.
  • Ready-to-use: Simplified protocol in which GrowDex hydrogels are mixed with media and cells.
  • Tunable: Optimal gel stiffness is achieved by dilution with cell culture media. Matrix composition can be optimized easily by supplementing cell culture media with specific biomolecules such as growth factors.
  • Compatible with high-content screening systems: No auto-fluorescence and transparency.

Why choose animal-free hydrogels for cell culture?

Despite being widely used, animal derived matrices are often poorly defined, contain contaminating molecules (RNA/DNA), suffer from lot-to-lot variability, and can be difficult to use. With animal free hydrogels, like GrowDex, you can benefit from:

  • Reproducible assays with no lot-to-lot variation
  • Temperature stability allowing room temperature handling
  • Defined composition without unknown protein composition

GrowDex

The original animal-free 3D hydrogel for cell culture, used in a wide range of cell-based assays. It has been validated on various cell models and applications.

GrowDex-T

  • Transparent hydrogel that has been specifically developed to offer superior imaging properties, without compromising any of the advantages of the original GrowDex hydrogel for 3D cell culture.
  • Ideal for use in brightfield, phase contrast and confocal microscopy applications as well as high content imaging.
  • GrowDex-A

    • Customizable transparent hydrogel, modified by the addition of an avidin group, that can be used to bind different biotinylated molecules.
    • Prescence of biotinylated molecules may enhance cell culture conditions for specific 3D applications.

    GrowDase

  • Cellulase enzyme that breaks down GrowDex hydrogel to form a solution in an easy one-step efficient cell recovery process, with no impact on cell viability or functionality.
  • Liberated cells can be used in gene or protein expression studies or for future experiments.
  • Products & Services (4)
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    • GrowDase

      GrowDase

      GrowDase enzyme breaks down GrowDex® hydrogel to form a solution in an easy one- step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.

      Price Range : 136.00 USD - 179.00 USD
    • GrowDex

      GrowDex®-A

      GrowDex®-A is a ready to use, transparent hydrogel that has been specifically developed to bind different biotinylated molecules, whose presence may enhance cell culture conditions for specific 3D applications. GrowDex-A consists of avidin conjugated nanofibrillar cellulose which can be customized by binding different biotinylated molecules e.g. proteins or peptides, to create a cell specific matrix.  Ambient handling and shear thinning properties of GrowDex-A enable easy dispensing and use in automated systems.

      Price Range : 218.00 USD - 565.00 USD
    • GrowDex-T Hydrogel - 2.5 ml

      GrowDex®-T

      GrowDex®-T is an animal-free ready to use hydrogel that has been specifically developed to offer superior imaging properties, but without compromising on any of the advantages of the original hydrogel GrowDex. GrowDex-T can be mixed directly with cells and culture media, no gelation or cross-linking step required. Ambient handling and shear thinning properties of GrowDex-T enable easy dispensing and use in automated systems.

      Price Range : 193.00 USD - 506.00 USD
    • GrowDex

      GrowDex®

      GrowDex® is an animal-free hydrogel that enables complete control over the culture environment for cells.

      GrowDex can be used in a wide range of cell-based assays such as microscopic imaging or metabolic activity measurement. GrowDex has been validated on various cell models and applications including primary hepatocytes, patient derived cells, 3D organoid formation, hepatocyte toxicity, stem cell proliferation and differentiation, tumour cell migration and invasion, neurite outgrowth and network formation.

      Price Range : 155.00 USD - 373.00 USD