GPCR research remains a widely studied area in drug discovery. As the complexity of the roles of GPCRs in signaling are unraveled, it is as important as ever to have reagents you can trust. We offer just that, with assay reagents for any GPCR target, including Gs-, Gq- and Gi-coupled receptors. Whether you are looking for functional or binding assays, our portfolio is sure to offer what you need.
Our GPCR research reagents portfolio offers solutions for identifying and monitoring a variety of GPCR research applications, including:
GPCR pathway activation via intracellular calcium, cAMP, phospho-ERK, inositol phosphates
Reporter gene expression in response to GPCR signaling
GTPγS binding activity
Receptor Cell Lines & Membranes
Our selection of GPCR cell lines and membranes allows you to decrease your assay development time and increase the quality of your results. Our cell lines and membrane preparation are validated for optimal performance in both function and binding assays.
Receptor binding studies are an integral part of GPCR research. Our radioligand are all tested specifically for receptor binding during development. Furthermore, many of our GPCR related radioligands are used to QC our GPCR cell lines and membrane preparations.
PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.
AlphaLISA SureFire Ultra
This assay platform for the detection of phosphoproteins in simple to complex matrices serves as an excellent secondary technology for challenging targets and provides an alternative for tough-to-screen targets not optimally coupled through the calcium or cAMP pathway
Glucagon-like peptide-1 (GLP-1), labeled with 125I. GLP-1 is a peptide hormone involved in secretion of insulin, and binds and activates GLP-1 receptors, a member of the glucagon receptor family of GPCRs.
Deoxycytidine triphosphate (dCTP) is a nucleoside triphosphate that is used whenever DNA is synthesized, such as in the polymerase chain reaction. 125I dCTP may be used to produce cDNA transcripts of viral or mRNA.
The AlphaLISA®SureFire®Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Our frozen, irradiated AequoZen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
AequoScreen Parental cell stably expressing the mitochondrially targeted apoaequorin gene. GPCRs of your choice can be transiently or stably transfected into these cell lines, for luminescence calcium assays.
Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.