As the understanding of the role of epigenetic changes in various disease states advances, the need for high quality reagents that can rapidly identify and validate therapeutic molecules that target these enzymes is increasing.
Our epigenetics assay portfolio includes:
Cellular detection kits – these AlphaLISA® kits enable rapid and direct detection of endogenous modifications of epigenetic marks on histon H3
In vitro assay toolbox – consists of validated off-the-shelf reagents for the detection of post-translational modifications at histone H3, H4 and p53
3H-SAM Methylation assay – enables both quantitative kinetic measure of substrate methylation rates and the measure of methyltransferases with low activity utilizing the gold standard reagent for the study of histone methylation – Adenosyl-L-Methionine, S-[methyl-3H]; (3H-SAM)
Benefits of epigenetic kits and assays include:
Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in biochemical or cell-based format
Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
Specificity: Reagents highly specific for the mark of interest
Simplicity: One-well, no-wash assay platforms amendable to automation and HTS
Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays
Whether you need an epigenetics kit or validated assay, our suite of epigenetic research reagents offers a faster, more efficient path in the race to identify promising epigenetic drug candidates.
AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.
AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 and H4 methylated on arginine residues. This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.
DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.