Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

Products & Services (287)
Technical Resources (327)
Tools & More (13)
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Products & Services (287)

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Product Type

Consumables (2)
Instruments (1)
Reagents (284)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (4)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (1)
AMPKα (2)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (1)
c-Met (2)
Chk-1 (1)
Chk-2 (1)
Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
ErbB2 (1)
ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
JNK1/2/3 (2)
JNK1/3 (1)
LCK (1)
MEK1 (1)
MKK3/6 (1)
MKK4 (3)
mTOR (2)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (4)
PDK1 (1)
S6RP (3)
SHP-1 (2)
SHP-2 (3)
SLP-76 (4)
SMAD1 (3)
SMAD2 (3)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (3)
Mouse (1)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (164)
Mobility Shift (36)
Protein Analysis & Detection (115)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (57)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (49)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (29)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (138)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (91)
TR-FRET (86)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (98)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay Points (200-50,000)

100 (51)
1000 (1)
10000 (119)
500 (125)
5000 (14)
50000 (94)

Assay Target Class

Histone (10)
Phosphoprotein (103)
Protein (29)

Automation Compatible

Yes (222)

Experimental Type

In vitro (153)

Format

Microplates (167)

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1-25 of 287 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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AlphaLISA Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

AL143R , AL143M , AL143C

AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.

Price Range : 1673.00 USD - 30500.00 USD
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Anti-unmodified H3K4 AlphaPlex^™-545 Acceptor Beads

AP119TB-R , AP119TB-M , AP119TB-C

AlphaPlex^™-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

Price Range : 1692.00 USD - 30800.00 USD
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AlphaScreen SureFire JNK 123 (p-Thr183;Tyr185) Assay Kit

TGRJPS500 , TGRJPS50K , TGRJPS10K

The AlphaScreen^® SureFire^® p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

AD0041 , AD0040

Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.

Price Range : 303.00 USD - 3531.00 USD
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LabChip ProfilerPro Separation Buffer (400 ml) including Coating Reagent 8, for EZ Reader

760367

Separation buffer containing coating reagent 8 (CR8), a positively-charged additive that binds the silanol groups of the glass channels in the LabChip multi-sipper chip (the glass is negatively-charged) to neutralize the charge. Non-specific interaction of substrates and products that are highly positively charged, or substrates with sequences containing repeats of a positive charge, will be reduced with this separation buffer.

List Price : 216.00 USD
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Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AL114C , AL114M , AL114R

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Price Range : 1741.00 USD - 31700.00 USD
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AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

TGRERS50K , TGRERS500 , TGRERS10K

The AlphaScreen^® SureFire^® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

AD0038 , AD0039

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.

Price Range : 353.00 USD - 3531.00 USD
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Quan-T-RT™ [³H] - Reverse Transcriptase

TRK1022

Quan-T-RT™ contains the following components: Annealed primer/template bound to streptavidin-coated SPA beads, assay buffer, stop solution, [3H]TTP (3.7 MBq, 100 µCi) and instructions for use.

List Price : 1152.00 USD
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ProfilerPro Kinase Selectivity Assay Plate Kit 1, for LabChip EZ Reader

760373

Kit consist of matched pairs of 384-well kinase-assay and substrate plates. Each assay plate contains 24 different predispensed frozen kinases. Substrate plates contain the appropriate fluorescent labeled peptides and ATP.

List Price : 1637.00 USD
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Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

ALSU-PAMPK-A500 , ALSU-PAMPK-A-HV , ALSU-PAMPK-A10K , ALSU-PAMPK-A50K

The AlphaLISA^® SureFire^® Ultra^™ p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaLISA SureFire Ultra Total SYK Assay Kit

ALSU-TSYK-A500 , ALSU-TSYK-A-HV , ALSU-TSYK-A10K , ALSU-TSYK-A50K

The AlphaLISA^® SureFire^® Ultra^™ Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 526.00 USD - 33600.00 USD
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AlphaScreen SureFire NFkB (p-Ser536) Assay Kit

TGRNFS50K , TGRNFS500 , TGRNFS10K

The AlphaScreen^® SureFire^® p-NF-κB p65 (p-Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NF-κB p65 (phosphorylated on Ser536) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

TRF0407-M , TRF0407-D

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3787.00 USD - 9500.00 USD
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Phospho-MKK4 (Ser257) AlphaLISA SureFire Ultra Detection Kit

ALSU-PMKK4-A50K , ALSU-PMKK4-A-HV , ALSU-PMKK4-A10K , ALSU-PMKK4-A500

The AlphaLISA^® SureFire^® Ultra™ phospho-MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated on Ser257) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD
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ProfilerPro Kinase Peptide Substrate 11, for LabChip EZ Reader

760355

Fluorescently-labelled peptide substrate 11 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

ALSU-PST4-A50K , ALSU-PST4-A-HV , ALSU-PST4-A10K , ALSU-PST4-A500

The AlphaLISA^® SureFire^® Ultra^™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

ALSU-PJNK-A500 , ALSU-PJNK-A-HV , ALSU-PJNK-A50K , ALSU-PJNK-A10K

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

TGRGBS500 , TGRGBS10K , TGRGBS50K

The AlphaScreen^® SureFire^® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

ALSU-PSLP-A500 , ALSU-PSLP-A50K , ALSU-PSLP-A-HV , ALSU-PSLP-A10K

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.

Price Range : 526.00 USD - 33600.00 USD
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LANCE Ultra - KinaSelect™ -TK Kit, 1,000 assay points

TRF0301-D

LANCE® Ultra TR-FRET tyrosine kinase assay kit.

List Price : 1638.00 USD
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Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

TRF4007M , TRF4007C

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.

Price Range : 1557.00 USD - 7000.00 USD
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AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

ALSU-PP70-A-HV , ALSU-PP70-A10K , ALSU-PP70-A50K , ALSU-PP70-A500

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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TREM2 LANCE Ultra TR-FRET Detection Kit

TRF4030C , TRF4030M

The LANCE Ultra TREM2 detection kit is designed for the detection of TREM2 in cell supernatants using a simple, homogeneous LANCE Ultra assay (no wash steps).

Price Range : 1557.00 USD - 7000.00 USD

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