Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

Products & Services (287)
Technical Resources (326)
Tools & More (13)
Business Insights (0)

Products & Services (287)

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Product Type

Consumables (2)
Instruments (1)
Reagents (284)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (4)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (1)
AMPKα (2)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (1)
c-Met (2)
Chk-1 (1)
Chk-2 (1)
Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
ErbB2 (1)
ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
JNK1/2/3 (2)
JNK1/3 (1)
LCK (1)
MEK1 (1)
MKK3/6 (1)
MKK4 (3)
mTOR (2)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (4)
PDK1 (1)
S6RP (3)
SHP-1 (2)
SHP-2 (3)
SLP-76 (4)
SMAD1 (3)
SMAD2 (3)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (3)
Mouse (1)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (164)
Mobility Shift (36)
Protein Analysis & Detection (115)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (57)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (49)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (29)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (138)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (91)
TR-FRET (86)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (98)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay Points (200-50,000)

100 (51)
1000 (1)
10000 (119)
500 (125)
5000 (14)
50000 (94)

Assay Target Class

Histone (10)
Phosphoprotein (103)
Protein (29)

Automation Compatible

Yes (222)

Experimental Type

In vitro (153)

Format

Microplates (167)

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1-25 of 287 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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AlphaScreen SureFire CREB (p-Ser133) Assay Kit

TGRCBS10K , TGRCBS500 , TGRCBS50K

The AlphaScreen^® SureFire^® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit

TGRCMS50K , TGRCMS10K , TGRCMS500

The AlphaScreen^® SureFire^® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1771.00 USD - 19100.00 USD
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AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

TGRJS50K , TGRJS500 , TGRJS10K

The AlphaScreen^® SureFire^® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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ProfilerPro Kinase Peptide Substrate 19, for LabChip EZ Reader

760363

Fluorescently-labelled peptide substrate 19 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

TGREB2S10K , TGREB2S500 , TGREB2S50K

The AlphaScreen^® SureFire^® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1771.00 USD - 19100.00 USD
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AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

TGRBCS10K , TGRBCS500 , TGRBCS50K

The AlphaScreen^® SureFire^® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

TGRKBS50K , TGRKBS500 , TGRKBS10K

The AlphaScreen^® SureFire^® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

ALSU-TZAP-A10K , ALSU-TZAP-A500 , ALSU-TZAP-A50K , ALSU-TZAP-A-HV

The AlphaLISA^® SureFire^® Ultra™ total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.

Price Range : 526.00 USD - 34900.00 USD
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AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

ALSU-PCREB-A50K , ALSU-PCREB-A-HV , ALSU-PCREB-A500 , ALSU-PCREB-A10K

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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LabChip ProfilerPro Separation Buffer (400 ml) including Coating Reagent 8, for EZ Reader

760367

Separation buffer containing coating reagent 8 (CR8), a positively-charged additive that binds the silanol groups of the glass channels in the LabChip multi-sipper chip (the glass is negatively-charged) to neutralize the charge. Non-specific interaction of substrates and products that are highly positively charged, or substrates with sequences containing repeats of a positive charge, will be reduced with this separation buffer.

List Price : 216.00 USD
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AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

TGRCHK1S10K , TGRCHK1S50K , TGRCHK1S500

The AlphaScreen^® SureFire^® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

TGRCOFS500 , TGRCOFS10K , TGRCOFS50K

The AlphaScreen^® SureFire^® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1771.00 USD - 19100.00 USD
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Phospho-SHP-1 (Tyr536) AlphaLISA SureFire Ultra Detection Kit

ALSU-PSHP1-A50K , ALSU-PSHP1-A-HV , ALSU-PSHP1-A10K , ALSU-PSHP1-A500

The AlphaLISA^® SureFire^® Ultra™ p-SHP-1 (Tyr536) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-1 (phosphorylated on Tyr536) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD
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LANCE Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

TRF0414-D , TRF0414-M

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine3 (H4R3me). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3787.00 USD - 9400.00 USD
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Phospho-Acetyl CoA Carboxylase (Ser79) AlphaLISA SureFire Ultra Assay Kit

ALSU-PACC-A50K , ALSU-PACC-A10K , ALSU-PACC-A500 , ALSU-PACC-A-HV

The AlphaLISA^® SureFire^® Ultra^™ p-ACC (Ser79) assay is a sandwich immunoassay for quantitative detection of phospho-acetyl CoA carboxylase (phosphorylated on Ser79) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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Phospho-SMAD2 (Ser465/467) AlphaLISA SureFire Ultra Detection Kit

ALSU-PSM2-A10K , ALSU-PSM2-A500 , ALSU-PSM2-A50K , ALSU-PSM2-A-HV

The AlphaLISA^® SureFire^® Ultra™ phospho-SMAD2 assay is a sandwich immunoassay for quantitative detection of SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD
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ProfilerPro Kinase Peptide Substrate 22, for LabChip EZ Reader

760366

Fluorescently-labelled peptide substrate 22 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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ProfilerPro Kinase Peptide Substrate 24, for LabChip EZ Reader

760387

Fluorescently-labelled peptide substrate 24 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

TRF0129-M , TRF0129-D

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA

Price Range : 538.00 USD - 956.00 USD
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AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

TGRERS50K , TGRERS10K , TGRERS500

The AlphaScreen^® SureFire^® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AL120R , AL120C , AL120M

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Price Range : 1673.00 USD - 30500.00 USD
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AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

TGRPS500 , TGRPS50K , TGRPS10K

The AlphaScreen^® SureFire^® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

TRF0203-C , TRF0203-D , TRF0203-M

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE

Price Range : 1665.00 USD - 7700.00 USD
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AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

ALSU-PST3-A50K , ALSU-PST3-A10K , ALSU-PST3-A-HV , ALSU-PST3-A500

The AlphaLISA^® SureFire^® Ultra^™ p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD

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