Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Technical Resources (324)

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Consumables (2)
Instruments (1)
Reagents (295)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (6)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (2)
AMPKα (2)
ATF1 (1)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (2)
c-Met (2)
Caspase-9 (1)
Chk-1 (1)
Chk-2 (1)
Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
Elk1 (1)
ErbB2 (1)
ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
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JNK1/3 (1)
LCK (1)
MEK1 (2)
MKK3/6 (1)
MKK4 (3)
mTOR (3)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (5)
PDK1 (1)
S6RP (3)
SHP-1 (2)
SHP-2 (1)
SLP-76 (4)
SMAD1 (3)
SMAD2 (4)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
Topo IIα (2)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (102)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (175)
Mobility Shift (36)
Protein Analysis & Detection (126)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (56)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (59)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (30)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (147)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (93)
TR-FRET (88)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (108)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay points(200-50,000)

100 (50)
1000 (1)
10000 (129)
500 (135)
5000 (14)
50000 (103)

Assay Target Class

Histone (10)
Phosphoprotein (114)
Protein (29)

Automation Compatible

Yes (234)

Experimental Type

In vitro (163)

Format

Microplates (177)

1-12 of 298 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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AlphaLISA Anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Acceptor Beads (for peptide substrate only)

AL123M , AL123R , AL123C

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 di-methylated at lysine 36 (H3K36me2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Price Range : 1581.00 USD - 27000.00 USD
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Phospho-Syk (Tyr352) AlphaLISA SureFire Ultra Detection Kit

ALSU-PSYK-B-HV , ALSU-PSYK-B10K , ALSU-PSYK-B500 , ALSU-PSYK-B50K

The AlphaLISA^® SureFire^® Ultra™ phospho-Syk assay is a sandwich immunoassay for quantitative detection of Syk (phosphorylated on Tyr352) in cellular lysates using Alpha Technology.

Price Range : 504.00 USD - 30400.00 USD
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AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

ALSU-PNFKB-A50K , ALSU-PNFKB-A500 , ALSU-PNFKB-A10K , ALSU-PNFKB-A-HV

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.

Price Range : 504.00 USD - 29200.00 USD
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AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

ALSU-PVGFR-A-HV , ALSU-PVGFR-A50K , ALSU-PVGFR-A500 , ALSU-PVGFR-A10K

The AlphaLISA^® SureFire^® Ultra^™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

Price Range : 504.00 USD - 29200.00 USD
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AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AL124C , AL124M , AL124R

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Price Range : 1481.00 USD - 27000.00 USD
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AlphaLISA Anti-O-linked GlcNAc Acceptor Beads

AL144C , AL144R , AL144M

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.

Price Range : 1481.00 USD - 27000.00 USD
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AlphaLISA dimethylated-Histone H3 Lysine 9 (H3K9me2) Cellular Detection Kit

AL717F , AL717HV , AL717C

This kit is designed for the detection of di-methylated Histone H3 Lysine 9 (H3K9me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Price Range : 725.00 USD - 12900.00 USD
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AlphaLISA SureFire Ultra Total SYK Assay Kit

ALSU-TSYK-A10K , ALSU-TSYK-A500 , ALSU-TSYK-A-HV , ALSU-TSYK-A50K

The AlphaLISA^® SureFire^® Ultra^™ Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 504.00 USD - 29200.00 USD
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AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

TGR70S50K , TGR70S500 , TGR70S10K

The AlphaScreen^® SureFire^® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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AlphaScreen SureFire Phospho-p38 MAPK Kit

TGR38S500 , TGR38S50K , TGR38S10K

The AlphaScreen^® SureFire^® p-38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Thr180/Tyr182) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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Phospho-AMPKα 1/2 (Thr172) AlphaLISA SureFire Ultra Assay Kit

ALSU-PAMPK-A-HV , ALSU-PAMPK-A500 , ALSU-PAMPK-A10K , ALSU-PAMPK-A50K

The AlphaLISA^® SureFire^® Ultra^™ p-AMPKα 1/2 (Thr172) assay is a sandwich immunoassay for quantitative detection of phospho-AMPKα (phosphorylated on Thr172) in cellular lysates using Alpha Technology.

Price Range : 504.00 USD - 29200.00 USD

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