Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

Products & Services (296)
Technical Resources (326)
Tools & More (13)
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Products & Services (296)

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Consumables (2)
Instruments (1)
Reagents (293)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (4)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (2)
AMPKα (2)
ATF1 (1)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (2)
c-Met (2)
Caspase-9 (1)
Chk-1 (1)
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Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
Elk1 (1)
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ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
JNK1/2/3 (2)
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LCK (1)
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MKK3/6 (1)
MKK4 (3)
mTOR (2)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (5)
PDK1 (1)
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SHP-1 (2)
SHP-2 (3)
SLP-76 (4)
SMAD1 (3)
SMAD2 (3)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
Topo IIα (2)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (99)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (173)
Mobility Shift (36)
Protein Analysis & Detection (124)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (57)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (58)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (29)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (147)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (91)
TR-FRET (86)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (107)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay Points (200-50,000)

100 (51)
1000 (1)
10000 (128)
500 (134)
5000 (14)
50000 (103)

Assay Target Class

Histone (10)
Phosphoprotein (112)
Protein (29)

Automation Compatible

Yes (231)

Experimental Type

In vitro (162)

Format

Microplates (176)

1-25 of 296 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac)

TRF0400-D , TRF0400-M

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

TGRS3S10K , TGRS3S50K , TGRS3S500

The AlphaScreen^® SureFire^® p-STAT3 (p-Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

TRF0406-M , TRF0406-D

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

TGRS6P2S10K , TGRS6P2S500 , TGRS6P2S50K

The AlphaScreen^® SureFire^® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

TRF0405-M , TRF0405-D

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaScreen SureFire STAT5 (p-Tyr694;Tyr699) Assay Kit

TGRS5S50K , TGRS5S500 , TGRS5S10K

The AlphaScreen^® SureFire^® p-STAT5 (p-Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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AlphaScreen SureFire JNK 123 (p-Thr183;Tyr185) Assay Kit

TGRJPS50K , TGRJPS10K , TGRJPS500

The AlphaScreen^® SureFire^® p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

TRF0404-M , TRF0404-D

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

TGRSM3S50K , TGRSM3S500 , TGRSM3S10K

The AlphaScreen^® SureFire^® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

TRF0412-M , TRF0412-D

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

AL723C , AL723F , AL723HV

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Price Range : 725.00 USD - 12900.00 USD
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AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

TGRIRS50K , TGRIRS500 , TGRIRS10K

The AlphaScreen^® SureFire^® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

TRF0407-D , TRF0407-M

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.

Price Range : 3632.00 USD - 9100.00 USD
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AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

TGRT2AS10K , TGRT2AS50K , TGRT2AS500

The AlphaScreen^® SureFire^® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1538.00 USD - 16600.00 USD
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Total MKK4 AlphaLISA SureFire Ultra Detection Kit

ALSU-TMKK4-A-HV , ALSU-TMKK4-A500 , ALSU-TMKK4-A50K , ALSU-TMKK4-A10K

The AlphaLISA^® SureFire^® Ultra™ total MKK4 assay is a sandwich immunoassay for quantitative detection of MKK4 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-MKK4 assay.

Price Range : 504.00 USD - 30400.00 USD
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AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

TGRS1S50K , TGRS1S10K , TGRS1S500

The AlphaScreen^® SureFire^® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

ALSU-PMTOR-A50K , ALSU-PMTOR-A-HV , ALSU-PMTOR-A10K , ALSU-PMTOR-A500

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.

Price Range : 504.00 USD - 29200.00 USD
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DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

AD0039 , AD0038

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.

Price Range : 339.00 USD - 3387.00 USD
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AlphaScreen SureFire CREB (p-Ser133) Assay Kit

TGRCBS10K , TGRCBS500 , TGRCBS50K

The AlphaScreen^® SureFire^® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1703.00 USD - 18200.00 USD
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AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

TGRJS50K , TGRJS10K , TGRJS500

The AlphaScreen^® SureFire^® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1538.00 USD - 16600.00 USD
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Total eIF2α AlphaLISA SureFire Ultra Detection Kit

ALSU-TEIF2-A-HV , ALSU-TEIF2-A500 , ALSU-TEIF2-A10K , ALSU-TEIF2-A50K

The AlphaLISA^® SureFire^® Ultra™ total eIF2α assay is a sandwich immunoassay for quantitative detection of eIF2α (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-eIF2α assay.

Price Range : 504.00 USD - 30400.00 USD
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AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

TGR703S10K , TGR703S50K , TGR703S500

The AlphaScreen^® SureFire^® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1538.00 USD - 16600.00 USD
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PDE Substrate iFL-cAMP, for LabChip EZ Reader

760444

Fluorescently-labelled cAMP for Phosphodiesterase Assays (Enyzme inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

TGRT2A2S50K , TGRT2A2S500 , TGRT2A2S10K

The AlphaScreen^® SureFire^® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1538.00 USD - 16600.00 USD

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