Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

Products & Services (287)
Technical Resources (326)
Tools & More (13)
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Products & Services (287)

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Product Type

Consumables (2)
Instruments (1)
Reagents (284)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (4)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (1)
AMPKα (2)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (1)
c-Met (2)
Chk-1 (1)
Chk-2 (1)
Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
ErbB2 (1)
ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
JNK1/2/3 (2)
JNK1/3 (1)
LCK (1)
MEK1 (1)
MKK3/6 (1)
MKK4 (3)
mTOR (2)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (4)
PDK1 (1)
S6RP (3)
SHP-1 (2)
SHP-2 (3)
SLP-76 (4)
SMAD1 (3)
SMAD2 (3)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (3)
Mouse (1)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (164)
Mobility Shift (36)
Protein Analysis & Detection (115)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (57)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (49)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (29)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (138)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (91)
TR-FRET (86)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (98)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay Points (200-50,000)

100 (51)
1000 (1)
10000 (119)
500 (125)
5000 (14)
50000 (94)

Assay Target Class

Histone (10)
Phosphoprotein (103)
Protein (29)

Automation Compatible

Yes (222)

Experimental Type

In vitro (153)

Format

Microplates (167)

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1-25 of 287 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

TGRMK4S500 , TGRMK4S50K , TGRMK4S10K

The AlphaScreen^® SureFire^® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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AlphaScreen SureFire p53 (p-Ser15) Assay Kit

TGRP53S50K , TGRP53S500 , TGRP53S10K

The AlphaScreen^® SureFire^® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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LabChip ProfilerPro Separation Buffer (400 ml) including Coating Reagent 8, for EZ Reader

760367

Separation buffer containing coating reagent 8 (CR8), a positively-charged additive that binds the silanol groups of the glass channels in the LabChip multi-sipper chip (the glass is negatively-charged) to neutralize the charge. Non-specific interaction of substrates and products that are highly positively charged, or substrates with sequences containing repeats of a positive charge, will be reduced with this separation buffer.

List Price : 216.00 USD
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Phospho-ZAP-70 (Tyr319) AlphaLISA SureFire Ultra Detection Kit

ALSU-PZAP-A500 , ALSU-PZAP-A50K , ALSU-PZAP-A-HV , ALSU-PZAP-A10K

The AlphaLISA^® SureFire^® Ultra™ phospho-ZAP-70 assay is a sandwich immunoassay for quantitative detection of ZAP-70 (phosphorylated on Tyr319) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD
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AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

TGRPS500 , TGRPS50K , TGRPS10K

The AlphaScreen^® SureFire^® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

TGRMK3S50K , TGRMK3S10K , TGRMK3S500

The AlphaScreen^® SureFire^® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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Total STAT3 LANCE Ultra TR-FRET Cellular Detection Kit

TRF4005C , TRF4005M

LANCE Ultra total STAT3 kits are designed for the detection of total STAT3 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-STAT3, and is compatible with both adherent and suspension cells.

Price Range : 1557.00 USD - 7000.00 USD
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ProfilerPro Kinase Peptide Substrate 8, for LabChip EZ Reader

760352

Fluorescently-labelled peptide substrate 8 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit

TGRP532S500 , TGRP532S10K , TGRP532S50K

The AlphaScreen^® SureFire^® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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Total AKT1/2/3 AlphaLISA SureFire Ultra Detection Kit

ALSU-TAKT-B500 , ALSU-TAKT-B50K , ALSU-TAKT-B-HV , ALSU-TAKT-B10K

The AlphaLISA^® SureFire^® Ultra™ total Akt1/2/3 assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Akt1/2/3 assay.

Price Range : 526.00 USD - 34900.00 USD
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AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

TGRS1S10K , TGRS1S500 , TGRS1S50K

The AlphaScreen^® SureFire^® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

ALSU-PST4-A-HV , ALSU-PST4-A500 , ALSU-PST4-A10K , ALSU-PST4-A50K

The AlphaLISA^® SureFire^® Ultra^™ p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

TGRS6PS50K , TGRS6PS500 , TGRS6PS10K

The AlphaScreen^® SureFire^® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.

Price Range : 1615.00 USD - 17400.00 USD
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Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

ALSU-PMTOR-A-HV , ALSU-PMTOR-A10K , ALSU-PMTOR-A500 , ALSU-PMTOR-A50K

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

ALSU-PERK-A-HV , ALSU-PERK-A50K , ALSU-PERK-A10K , ALSU-PERK-A500

The AlphaLISA^® SureFire^® Ultra^™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

ALSU-P4EBP-A10K , ALSU-P4EBP-A-HV , ALSU-P4EBP-A50K , ALSU-P4EBP-A500

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

ALSU-PVGFR-A-HV , ALSU-PVGFR-A50K , ALSU-PVGFR-A10K , ALSU-PVGFR-A500

The AlphaLISA^® SureFire^® Ultra^™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 33600.00 USD
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ProfilerPro Kinase Peptide Substrate 12, for LabChip EZ Reader

760356

Fluorescently-labelled peptide substrate 12 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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ProfilerPro Kinase Peptide Substrate 27, for LabChip EZ Reader

760424

Fluorescently-labelled peptide substrate 27 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

TRF0216-D , TRF0216-M

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)

Price Range : 2220.00 USD - 7700.00 USD
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LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

TRF0201-D , TRF0201-C , TRF0201-M

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK

Price Range : 319.00 USD - 6500.00 USD
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AlphaScreen SureFire STAT3 (p-Tyr705) Assay Kit

TGRS3S500 , TGRS3S10K , TGRS3S50K

The AlphaScreen^® SureFire^® p-STAT3 (p-Tyr705) assay is a sandwich immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1788.00 USD - 19100.00 USD
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AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

ALSU-TSLP-A500 , ALSU-TSLP-A50K , ALSU-TSLP-A-HV , ALSU-TSLP-A10K

The AlphaLISA^® SureFire^® Ultra^™ Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 526.00 USD - 33600.00 USD
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Phospho-SMAD2 (Ser465/467) AlphaLISA SureFire Ultra Detection Kit

ALSU-PSM2-A10K , ALSU-PSM2-A500 , ALSU-PSM2-A-HV , ALSU-PSM2-A50K

The AlphaLISA^® SureFire^® Ultra™ phospho-SMAD2 assay is a sandwich immunoassay for quantitative detection of SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD

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