Enzymology

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Find the end-point and real-time kinetic reagents and instruments you need for your enzymatic assays. We offer:

Radiolabeled substrates for highly-sensitive kinetic assays and antibody-free screening
TR-FRET, TRF, and Alpha no-wash technologies for fast and simple measurement of biochemical and cellular enzymatic activity
Microfluidic mobility-shift assays (MSA) for radiometric analysis of substrate conversion, kinetics, and mechanism-of-action
Multi-mode plate readers for maximal speed and throughput independent of technology platform

Products & Services (296)
Technical Resources (322)
Tools & More (13)
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Products & Services (296)

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Consumables (2)
Instruments (1)
Reagents (293)

Assay Target

4EBP1 (3)
Acetyl CoA carboxylase (1)
Akt1 (6)
Akt1/2 (1)
Akt1/2/3 (7)
ALK (2)
AMPKα (2)
ATF1 (1)
ATF2 (1)
BAD (2)
BTK (2)
c-Jun (2)
c-Met (2)
Caspase-9 (1)
Chk-1 (1)
Chk-2 (1)
Cofilin (1)
CREB (2)
EGFR (6)
eIF2α (2)
eIF4E (2)
Elk1 (1)
ErbB2 (1)
ERK1/2 (4)
GAPDH (2)
GSK3α (1)
GSK3β (2)
Histone H2A (1)
Histone H3 (10)
Hsp27 (1)
IKKα (3)
INSR (2)
IκBα (1)
JNK1/2/3 (2)
JNK1/3 (1)
LCK (1)
MEK1 (2)
MKK3/6 (1)
MKK4 (3)
mTOR (2)
NFκB (5)
p38 MAPK (1)
p53 (2)
p70 S6K (5)
PDK1 (1)
S6RP (3)
SHP-1 (2)
SHP-2 (1)
SLP-76 (4)
SMAD1 (3)
SMAD2 (3)
SMAD3 (3)
Src (2)
STAT1 (3)
STAT3 (5)
STAT4 (1)
STAT5 (3)
STAT6 (1)
SYK (3)
TBK1 (1)
Topo IIα (2)
TREM2 (1)
VEGF2 Receptor (3)
VEGFR2 (2)
Zap70 (4)
β-Catenin (1)

Target Species

Human (101)

Application

Drug Discovery & Development (3)
Epigenetics (46)
Kinase (173)
Mobility Shift (36)
Protein Analysis & Detection (124)

Therapeutic Area

Epigenetics (10)

Product Brand Name

AlphaLISA (31)
AlphaLISA SureFire Ultra (55)
AlphaPlex (1)
AlphaScreen (3)
AlphaScreen SureFire (58)
DELFIA (5)
LabChip EZ Reader (50)
LANCE (5)
LANCE Ultra (30)
LANCE Ultra (50)
SPA Enzymatic Assays (3)

Detection Method

Alpha (145)
DELFIA TRF (5)
Fluorescence (1)
Radiometric (4)
Time-Resolved Fluorescence (TRF) (93)
TR-FRET (88)

Molecular Modification

Acetylation (12)
GlcNAc (1)
Glycosidation (1)
Methylation (25)
Phosphorylation (107)
Total (1)
Unmodified (4)

Sample Type

Enzyme (38)

Antibody Conjugates

Anti-acetyl-lysine (1)
Anti-Acetyl-Lysine (1)
Anti-H3 (C-term) (1)
Anti-H3K27ac (2)
Anti-H3K27me2 (1)
Anti-H3K27me2-1 (1)
Anti-H3K27me3 (2)
Anti-H3K36me2 (2)
Anti-H3K4 (3)
Anti-H3K4me1-2 (2)
Anti-H3K9/H3K27 (1)
Anti-H3K9/K27 (1)
Anti-H3K9ac (2)
Anti-H3K9me2 (2)
Anti-H3R2me (1)
Anti-H4K20me1 (1)
Anti-H4R3me (2)
Anti-methyl arginine (1)
Anti-methyl-Arg (1)
Anti-O-GlcNAc (1)
Anti-O-linked-GlcNAc (1)
Anti-P-Ser 14-3-3 (1)
Anti-P-Tyr-100 (1)
Anti-p53K382ac (2)
Anti-phospho-4EBP1 (1)
Anti-phospho-Acetyl-CoA-Carboxylase (1)
Anti-phospho-Crosstide (1)
Anti-phospho-glycogen synthase (1)
Anti-phospho-Histone H3 (2)
Anti-phospho-I?Ba (1)
Anti-phospho-MEK1/2 (1)
Anti-phospho-p70S6K (1)
Anti-phospho-PKC (1)
Anti-phospho-PLK (1)
Anti-phospho-rpS6 (1)
Anti-phospho-serine (1)
Anti-phospho-threonine (2)
Anti-phospho-Topo IIa (1)
Anti-phospho-Tyrosine Hydroxylase (1)
P-Tyr-100 (1)
PT66 (2)
PY20 (2)
Streptavidin/Phosphosensor (1)
Streptavidin/PT66 (1)
Streptavidin/PY20 (1)

Radioisotope

H-3 (3)

Fluorophore

Eu-N1 (5)
Eu-W1024 (37)
ULight (18)

Assay points(200-50,000)

100 (49)
1000 (1)
10000 (127)
500 (133)
5000 (14)
50000 (101)

Assay Target Class

Histone (10)
Phosphoprotein (112)
Protein (29)

Automation Compatible

Yes (232)

Experimental Type

In vitro (162)

Format

Microplates (176)

1-12 of 296 Products & Services

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EZ READER, SHIP LEVEL

122919

The EZ Reader can help to save time and money in your laboratory by identifying the most promising lead candidates faster.

The EZ Reader instrument is available outside the EU. Please contact your local representative for further information.
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AlphaLISA SureFire Ultra Total mTOR Assay Kit

ALSU-TMTOR-A500 , ALSU-TMTOR-A10K , ALSU-TMTOR-A-HV , ALSU-TMTOR-A50K

The AlphaLISA^® SureFire^® Ultra^™ Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 504.00 USD - 29200.00 USD
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Phospho-STAT1 (Tyr701) LANCE Ultra TR-FRET Detection Kit

TRF4028M , TRF4028C

LANCE Ultra phospho-STAT1 (Tyr701) kits are designed for the detection of phosphorylated STAT1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

Price Range : 1512.00 USD - 6800.00 USD
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AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AL124R , AL124M , AL124C

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

Price Range : 1481.00 USD - 27000.00 USD
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ProfilerPro Kinase Peptide Substrate 19, for LabChip EZ Reader

760363

Fluorescently-labelled peptide substrate 19 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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Biotin-anti-H3K79me2 antibody

AL148M , AL148C , AL148R

Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.

Price Range : 1481.00 USD - 27000.00 USD
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AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

TGRCHK1S10K , TGRCHK1S500 , TGRCHK1S50K

The AlphaScreen^® SureFire^® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1538.00 USD - 16600.00 USD
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AlphaLISA Anti-unmodified Histone H3 Lysine 4 (H3K4) Acceptor Beads

AL119R , AL119M , AL119C

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 unmodified against lysine 4 (H3K4). These beads can be used for no-wash AlphaLISA epigenetic eraser assays.

Price Range : 1481.00 USD - 27000.00 USD
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ProfilerPro Kinase Peptide Substrate 15, for LabChip EZ Reader

760359

Fluorescently-labelled peptide substrate 15 for kinase assays (inhibiton, target discovery, lead optimization)

List Price : 757.00 USD
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Phospho-c-Met (Tyr1234/1235) AlphaLISA SureFire Ultra Assay Kit

ALSU-PCMET-A50K , ALSU-PCMET-A500 , ALSU-PCMET-A-HV , ALSU-PCMET-A10K

The AlphaLISA^® SureFire^® Ultra^™ p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.

Price Range : 504.00 USD - 29200.00 USD
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AlphaLISA SureFire Ultra Total Akt1 Assay Kit

ALSU-TAKT1-A-HV , ALSU-TAKT1-A10K , ALSU-TAKT1-A50K , ALSU-TAKT1-A500

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 504.00 USD - 29200.00 USD
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Phospho-TBK1 (Ser172) LANCE Ultra TR-FRET Detection Kit

TRF4031C , TRF4031M

LANCE Ultra phospho-TBK1 (Ser172) kits are designed for the detection of phosphorylated TBK1 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

Price Range : 1512.00 USD - 6800.00 USD

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