PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1525 Products & Services

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  • AlphaLISA immunoassay kits

    IL-18 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine IL-18 enables the quantitative determination of porcine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 451.00 USD - 9900.00 USD
  • SureFire principle

    AlphaScreen SureFire p53 (p-Ser15) Assay Kit

    The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLISA immunoassay kits

    IFN-γ (ovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for ovine interferon gamma (IFNγ) enables the quantitative determination of ovine IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 451.00 USD - 9900.00 USD
  • AlphaLISA immunoassay kits

    EGF (human) AlphaLISA Detection Kit

    The AlphaLISA® EGF Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor (EGF) in cell culture media, serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 672.00 USD - 10500.00 USD
  • LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3632.00 USD - 9100.00 USD
  • AlphaLISA immunoassay kits

    IL-18BP (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human IL-18BP enables the quantitative determination of human interleukin 18-binding protein in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11600.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-PDK 1 (Ser241) Kit

    The AlphaScreen® SureFire® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLisa Analyte 2

    CXCL4/PF4 (Human) lyophilized (AlphaLISA)

    Human CXCL4/PF4 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA CXCL4/PF4 Detection Kit, but can be ordered separately.
    List Price : 317.00 USD
  • AlphaLISA immunoassay kits

    IL-8 (bovine/ovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.
    Price Range : 724.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    EGFR (human) AlphaLISA Detection Kit

    The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 724.00 USD - 10500.00 USD
  • IL-8 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human IL-8 Detection Kit is designed for detection and quantitation of human interleukin 8 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • AlphaLISA immunoassay kits

    Cry1Ab/1Ac AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for Cry1Ab/1Ac enables the detection and quantitation of Cry1Ab or Cry1Ac in plant seed and leaf extracts using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 607.00 USD - 9400.00 USD
  • AlphaLisa Analyte 2

    IL-2 (bovine), lyophilized (AlphaLISA)

    Bovine IL-2 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Bovine IL-2 Detection Kit, but can be ordered separately.
    List Price : 250.00 USD
  • AlphaLISA immunoassay kits

    Leptin (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Leptin Detection Kit is designed for detection and quantitation of human leptin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Due to the presence of leptin binding proteins in serum or other matrices, an acid-dissociation step might be required before performing the AlphaLISA assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • SureFire principle

    AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

    The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3632.00 USD - 9100.00 USD
  • SureFire principle

    AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

    The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • AlphaLISA immunoassay kits

    FcγR3B (CD16b) AlphaLISA Binding Kit

    The AlphaLISA FCGR3B (CD16b) binding kit is designed for the detection of binding between FcγR3B (CD16b) and human IgG Fc fragment using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapeutics using a competition assay format.
    Price Range : 2213.00 USD - 11100.00 USD
  • AlphaLISA SureFire Ultra kits

    Total MEK1 AlphaLISA SureFire Ultra Assay Kit

    The AlphaLISA® SureFire® Ultra™ Total MEK1 assay is a sandwich immunoassay for quantitative detection of MEK1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
    Price Range : 504.00 USD - 29200.00 USD
  • LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody

    Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.
    Price Range : 3632.00 USD - 9100.00 USD
  • SureFire principle

    AlphaScreen SureFire STAT1 (p-Tyr701) Assay Kit

    The AlphaScreen® SureFire® p-STAT1 (p-Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
    Price Range : 1597.00 USD - 7400.00 USD
  • AlphaLisa Analyte 2

    IL-6 (mouse), lyophilized (AlphaLISA)

    Mouse Interleukin-6 (IL-6) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-6 kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire® Ultra™ phospho-PD-1 (pan Tyr) Assay Kit

    The AlphaLISA® SureFire® Ultra™ phospho-PD-1 (pan Tyr) assay kit can be used to measure levels of PD-1 (phosphorylated on tyrosine residues) in cellular lysates using no-wash Alpha technology.
    Price Range : 504.00 USD - 30400.00 USD
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