PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1512 Products & Services

  • Protein

    GLP-1 (7-36) (human), lyophilized (LANCE)

    Human GLP-1 (7-36) standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human GLP-1 (7-36) TR-FRET Detection Kit, but can be ordered separately.
    List Price : 261.00 USD
  • AlphaLISA immunoassay kits

    IgA (mouse) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 738.00 USD - 11500.00 USD
  • SureFire principle

    AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

    The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaLISA immunoassay kits

    IgG (mouse) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of mouse IgG in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 829.00 USD - 11500.00 USD
  • AlphaLisa Analyte 2

    Mesothelin (human), lyophilized (AlphaLISA)

    Human mesothelin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Mesothelin Detection Kit, but can also be ordered separately.
    List Price : 481.00 USD
  • AlphaLISA immunoassay kits

    MMP-12 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.
    Price Range : 531.00 USD - 11600.00 USD
  • IgG1 (bovine) AlphaLISA Detection Kit

    The AlphaLISA® Bovine IgG1 Detection Kit is designed for detection, quantitation, and isotyping of bovine IgG1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 799.00 USD - 12800.00 USD
  • SureFire principle

    AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit

    The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1788.00 USD - 19100.00 USD
  • AlphaLISA binding assay

    PDGF-BB/PDGFRα (human) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRα, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRα.
    Price Range : 2082.00 USD - 16700.00 USD
  • LANCE Ultra Europium-anti-phospho-CREB (Ser133)

    Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
    Price Range : 1210.00 USD - 5200.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • IL-1ß (rat) AlphaLISA Detection Kit

    The AlphaLISA® Rat Interleukin 1ß (IL-1ß) Detection Kit is designed for detection and quantitation of rat IL-1ß in bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

    Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.
    Price Range : 3787.00 USD - 9500.00 USD
  • Fc-fragment (human) conjugated AlphaLISA Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to human IgG Fc fragment. These beads can be used to capture Fc binding proteins and receptors.
    Price Range : 920.00 USD - 28000.00 USD
  • AlphaLisa Analyte 2

    IFN-γ (bovine), lyophilized (AlphaLISA)

    Bovine IFNγ standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IFNγ kits, but can also be ordered separately.
    List Price : 399.00 USD
  • AlphaLISA immunoassay kits

    IgG Fc (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for detection and quantitation of human IgG Fc in buffer or cell culture supernatant allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development. The antibodies in the kit target the Fcγ region of human IgG.
    Price Range : 1313.00 USD - 10600.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-VEGF Receptor 2 (Tyr1175) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-VEGF Receptor 2 (Tyr1175) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA binding assay

    HLA-DRA/LAG3 (human) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between Human HLA-DRA and LAG3, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to HLA-DRA and LAG3.
    Price Range : 2082.00 USD - 16700.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

    The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.
    Price Range : 526.00 USD - 33600.00 USD
  • ULight anti-human IgG

    ULight™ labeled anti-human IgG antibody for LANCE® TR-FRET assays. The anti-human IgG antibody is a purified goat polyclonal antibody, and reacts with whole molecule human IgG.
    Price Range : 465.00 USD - 11800.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

    Total NFκB LANCE Ultra TR-FRET Detection Kit

    LANCE Ultra total NFκB kits are designed for the detection of NFκB (both phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra TR-FRET assay (no wash steps). This assay is compatible with both adherent and suspension cells, and is intended to be used as a control or normalization assay in conjunction with the LANCE phospho-NFκB assay.
    Price Range : 1557.00 USD - 7000.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-PLK (Ser137) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRRSLLE
    Price Range : 251.00 USD - 910.00 USD
  • AlphaLISA immunoassay kits

    IL-5 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse IL-5 enables the quantitative determination of mouse interleukin-5 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 715.00 USD - 17200.00 USD
  • AlphaLISA SureFire Ultra kits

    Phospho-ErbB2 (Tyr1221/1222) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
    Price Range : 526.00 USD - 33600.00 USD
  • Unconjugated AlphaLISA Acceptor beads

    Unconjugated AlphaLISA Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaLISA Acceptor beads for use in Alpha no-wash assays.
    Price Range : 1600.00 USD - 33600.00 USD
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