PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-12 of 1485 Products & Services

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  • AlphaLISA immunoassay kits

    IL-33 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human IL-33 enables the quantitative determination of human interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • AlphaLisa Analyte 2

    TDP-43 (human), lyophilized (AlphaLISA)

    Human TDP-43 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA TDP-43 Detection Kit, but can be ordered separately.
    List Price : 322.00 USD
  • Protein

    TSH (human), lyophilized (LANCE)

    Human thyroid stimulating hormone (TSH) standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human TSH Detection Kit, but can also be ordered separately.
    List Price : 250.00 USD
  • AlphaLISA immunoassay kits

    PDGF (BB dimer) (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

    Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

    LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
    Price Range : 1512.00 USD - 6800.00 USD
  • LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
    Price Range : 2130.00 USD - 7400.00 USD
  • IL-25 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • AlphaPlex-545-unconjugated

    Unconjugated AlphaPlex-545 Acceptor Beads

    Unconjugated AlphaPlex 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
    Price Range : 1400.00 USD - 29500.00 USD
  • AlphaLisa Analyte 2

    IL-6 (bovine), lyophilized (AlphaLISA)

    Bovine IL6 standard, 1 µg, for use in AlphaLISA no-wash immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IL6 kits, but can also be ordered separately as needed.
    List Price : 257.00 USD
  • AlphaLISA immunoassay kits

    FSH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 541.00 USD - 16200.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total NFκB Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-NFκB p65 (Ser536) + Total NFκB p65 assay kit is used to measure both the phosphorylation (on Ser536) and total levels of endogenous NFκB in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure NFκB phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
  • AlphaLISA immunoassay kits

    FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 451.00 USD - 9900.00 USD
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