PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1524 Products & Services

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  • ULight anti-FLAG

    ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
    Price Range : 453.00 USD - 11100.00 USD
  • AlphaLISA immunoassay kits

    Adiponectin (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Adiponectin Detection Kit is designed for detection and quantitation of human adiponectin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight-p70 S6K (Thr389) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
    Price Range : 306.00 USD - 1250.00 USD
  • AlphaLisa Analyte 2

    CCL5/RANTES (human), lyophilized (AlphaLISA)

    Human C-C Motif Chemokine 5 / Regulated Upon Activation Normal T-cell Expressed and Secreted (CCL5 / RANTES) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CCL5/RANTES kit, but can be ordered separately.
    List Price : 552.00 USD
  • IL-6 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 6 (IL-6) enables the quantitative determination of porcine IL-6 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • Alpha donor antibody coated

    Anti-Mouse IgG Alpha Donor beads

    Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
    Price Range : 502.00 USD - 9100.00 USD
  • PerkinElmer

    LANCE Ultra ULight™-MBP peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
    Price Range : 330.00 USD - 900.00 USD
  • SureFire principle

    AlphaScreen SureFire Akt 1/2 (p-Ser473) Assay Kit

    The AlphaScreen® SureFire® p-Akt 1/2 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt 1/2 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLISA immunoassay kits

    EPO (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Erythropoietin (EPO) Detection Kit is designed for detection and quantitation of human EPO in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3036.00 USD - 23700.00 USD
  • AlphaLISA immunoassay kits

    IFN-γ (mouse) AlphaLISA Detection Kit

    The AlphaLISA® Mouse Interferon-γ (IFNγ) Detection Kit is designed for detection and quantitation of mouse IFNγ in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLISA SureFire Ultra kits

    Total SMAD2 AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total SMAD2 assay is a sandwich immunoassay for quantitative detection of total SMAD2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-SMAD2.
    Price Range : 504.00 USD - 30400.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif: RRQSLIE
    Price Range : 320.00 USD - 873.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-BAD (Ser136) Kit

    The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLISA immunoassay kits

    Cry1F AlphaLISA Detection Kit

    The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 625.00 USD - 9700.00 USD
  • AlphaPlex-545-biomarker

    AlphaPlex-545 (Tb) TruHits Kit

    Control beads for identifying false positives and interfering compounds in AlphaPlex multiplexing no-wash assays.
    Price Range : 596.00 USD - 4138.00 USD
  • PerkinElmer

    LANCE Eu-W8044 Streptavidin

    Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays. The W8044 chelate provides more stability than the W1024 chelate.
    Price Range : 1087.00 USD - 9500.00 USD
  • AlphaLISA immunoassay kits

    IL-33 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • IL-13 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse IL-13 enables the quantitative determination of mouse interleukin-13 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human IL2 Detection Kit

    The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 633.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    IL-8 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

    Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
    Price Range : 1597.00 USD - 7400.00 USD
  • SureFire principle

    AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

    The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • Alpha_Strep-Tactin_Donor_bead

    Strep-Tactin® Alpha Donor beads

    Alpha Donor beads conjugated to Strep-Tactin®. These beads can be used to capture Strep-tag® II-tagged proteins for Alpha no-wash assays.
    Price Range : 502.00 USD - 9100.00 USD
  • AlphaLisa Analyte 2

    CRP (human), lyophilized (AlphaLISA)

    Human C-Reactive Protein (CRP) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CRP kit, but can be ordered separately.
    List Price : 257.00 USD
  • LANCE Ulight labeled peptide

    LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence: CSERRPHFPQFSYSASGTAR
    Price Range : 306.00 USD - 1250.00 USD
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