PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1424 Products and Services

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AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
Price Range : 180.00 USD - 13800.00 USD

CYR61 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cysteine rich angiogenic inducer 61 (CYR61) enables the quantitative determination of human Cyr61 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 
Price Range : 192.00 USD - 13800.00 USD

LDHA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lactate dehydrogenase subunit A (LDHA) enables the quantitative determination of human LDHA in cell lysates and serum using a homogeneous AlphaLISA assay (no wash steps). Please note this is NOT a cell cytotoxicity assay, as the assay does not measure secreted LDH.
Price Range : 728.00 USD - 14000.00 USD
AlphaLISA immunoassay kits

C-peptide (human) AlphaLISA Kit

AlphaLISA® no-wash immunoassay kit for detection of human C-peptide (human connecting peptide) in serum, buffered solution or cell culture medium.
Price Range : 1476.00 USD - 9800.00 USD
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 489.00 USD - 28400.00 USD

IL-8 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 8 (IL-8) enables the quantitative determination of porcine IL-8 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1064.00 USD - 10300.00 USD
AlphaLisa Analyte 2

IFN-β (human), lyophilized (AlphaLISA)

Human Interferon β (IFNβ) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. The standard corresponds to the IFN-ß 1a subtype . This standard is already provided in the AlphaLISA human IFNβ kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLisa Analyte 2

MBNL1 (human), lyophilized (AlphaLISA)

Human muscleblind-like protein 1 (MBNL1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human MBNL1 Detection Kit, but can also be ordered separately.
List Price : 760.00 USD

BCL-2 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human BCL-2 Detection Kit is designed for detection and quantitation of human Bcl-2 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 796.00 USD - 10600.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IKKα (Ser176/180) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA immunoassay kits

Utrophin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human utrophin enables the quantitative determination of utrophin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 288.00 USD - 18000.00 USD
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLisa Analyte 2

IgM (human), lyophilized (AlphaLISA)

Human IgM standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IgM kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA SureFire Ultra Lysate

AlphaLISA SureFire Ultra SMAD1 pS463/465 Lysate

Extra positive and negative control lysates for the AlphaLISA SureFire Ultra phospho-Smad1 (Ser463/465) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-Smad1 assay kit, but can also be ordered separately.
List Price : 21.00 USD
AlphaLISA immunoassay kits

IL-4 (rhesus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rhesus interleukin-4 (IL-4) enables the quantitative determination of rhesus IL-4 in buffered solution, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
SureFire principle

AlphaScreen SureFire ATF1 (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

AlphaScreen Mouse IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.
Price Range : 180.00 USD - 13800.00 USD

Anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 and H4 methylated on arginine residues. This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1448.00 USD - 25900.00 USD
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of human TNFα in serum, buffered solution or cell culture medium.
Price Range : 1520.00 USD - 10100.00 USD

MMP-1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Matrix Metalloproteinase-1 (MMP1) Detection Kit is designed for detection and quantitation of human MMP1 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP1 and pro-MMP1.
Price Range : 1520.00 USD - 10100.00 USD
AlphaLISA immunoassay kits

PON-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human paraoxonase/arylesterase 1 (PON-1) enables the quantitative determination of human PON-1 in buffered solution, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 728.00 USD - 14000.00 USD
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 1043.00 USD - 9100.00 USD
AlphaLISA immunoassay kits

Beta-2 Microglobulin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human beta-2 microglobulin enables the quantitative determination of human beta-2 microglobulin in buffered solution, serum, CSF, urine, saliva, cell culture supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 1747.00 USD - 14000.00 USD
SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA immunoassay kits

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT4 (Tyr693) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT4 (Tyr693) assay is a sandwich immunoassay for quantitative detection of endogenous STAT4 phosphorylated at Tyr693 in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLisa Analyte 2

IL-11 (human), lyophilized (AlphaLISA)

Human Interleukin 11 (IL-11) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human IL-11 kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLisa Analyte 2

IGF-1 (rat), lyophilized (AlphaLISA)

Rat IGF-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Rat IGF-1 Detection Kit, but can also be ordered separately.
List Price : 295.00 USD
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 1343.00 USD - 28300.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Samarium Acceptor Beads

Streptavidin AlphaPlex™ 645 (Sm) Acceptor Beads

The Streptavidin coated AlphaPlex™ 645 (Samarium) Acceptor beads are used to capture a wide variety of biotinylated reagents, ranging from small nucleotides to very large proteins. To avoid steric hindrance, it is recommended to use biotin derivatives including an aliphatic spacer composed of at least six carbons.
Price Range : 587.00 USD - 24100.00 USD
AlphaLISA immunoassay kits

HLA-C (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLisa Analyte 2

IL-2 (human), lyophilized (AlphaLISA)

Human Interleukin-2 (IL2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL-2 kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA SureFire Ultra kits

Total STAT3 AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 489.00 USD - 28400.00 USD
AlphaLISA immunoassay kits

GLP-1 (7-36) (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human GLP-1 enables the detection and quantitation of human GLP-1 (7-36 amide) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 695.00 USD - 9700.00 USD
Protein

IL-12/p40 (human), lyophilized (LANCE)

Human IL-12/p40 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-12/p40 Detection Kit, but can also be ordered separately.
List Price : 240.00 USD
AlphaPlex-545-antibody-bead2

Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.
Price Range : 1421.00 USD - 25800.00 USD
AlphaLISA binding assay

PDGF-BB/PDGFRβ (human) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human PDGF-BB and PDGFRβ, using a homogeneous AlphaLISA assay (no wash step). This assay can facilitate the design and development of antibody therapeutics by using competitive binding to PDGF-BB and PDGFRβ.
Price Range : 1747.00 USD - 14000.00 USD

ULight anti-c-myc antibody

ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
Price Range : 428.00 USD - 10800.00 USD
LANCE Detection Kit

PIP Collagen (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of human PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 773.00 USD - 10300.00 USD
AlphaLISA immunoassay kits

LAG-3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human LAG-3 enables the quantitative determination of human lymphocyte-activation gene 3 (LAG-3) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

LANCE Eu-W1024-ITC Chelate and Eu Standard, 1 mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in LANCE TR-FRET assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
List Price : 13000.00 USD

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 433.00 USD - 9500.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IKKα (Ser176/180) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
AlphaLisa Analyte 2

IFN-γ (cynomolgus), lyophilized (AlphaLISA)

Cynomolgus IFNγ standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Cynomolgus IFNγ Detection Kit, but can also be ordered separately.
List Price : 233.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD

Anti-bovine IgG2 AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-bovine IgG2 antibody. These beads can be used to capture bovine IgG2 antibodies.
Price Range : 828.00 USD - 28000.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IP10 Detection Kit

The AlphaPlex Terbium (Tb) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 688.00 USD - 10000.00 USD

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