PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1517 Products & Services

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  • MMP-3 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human MMP-3 Detection Kit is designed for detection and quantitation of human matrix metalloproteinase-3 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 829.00 USD - 11100.00 USD
  • AlphaLISA immunoassay kits

    IFNβ (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interferon β (IFNβ) enables the quantitative determination of porcine IFNβ in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • LANCE Detection Kit

    PAI-1 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human PAI-1 Detection Kit is designed for detection and quantitation of human PAI-1 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11100.00 USD
  • LANCE Ultra ULight™-IRS-1 (Tyr983) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence: CKKSRGDYMTMQIG
    Price Range : 515.00 USD - 1250.00 USD
  • AlphaLISA immunoassay kits

    TNFR1 (mouse, soluble) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for soluble mouse tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of soluble mouse TNFR1 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11100.00 USD
  • AlphaLisa Analyte 2

    GLP-1 (7-36) (human), lyophilized (AlphaLISA)

    Human GLP-1 (7-36) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human GLP-1 Detection Kit, but can be ordered separately.
    List Price : 250.00 USD
  • Protein

    PAI-1 (human), lyophilized (LANCE)

    Human PAI-1 standard, lyophilized, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human PAI-1 TR-FRET Detection Kit, but can be ordered separately.
    List Price : 250.00 USD
  • AlphaLISA immunoassay kits

    pNFH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human phosphorylated neurofilament heavy chain protein (pNFH) enables the quantitative determination of human pNFH in plasma or cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 750.00 USD - 11100.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaScreen cAMP assay principle

    AlphaScreen cAMP Detection Kit

    AlphaScreen no-wash assay kit for quantitative detection of cAMP.
    Price Range : 420.00 USD - 14400.00 USD
  • IL-4 (rhesus) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for rhesus interleukin-4 (IL-4) enables the quantitative determination of rhesus IL-4 in buffered solution, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 451.00 USD - 9900.00 USD
  • AlphaLisa Analyte 2

    IFNβ (porcine), lyophilized (AlphaLISA)

    Porcine interferon β (IFNβ) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IFNβ Detection Kit, but can also be ordered separately.
    List Price : 736.00 USD
  • AlphaLISA immunoassay kits

    IL-15 (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 465.00 USD - 10200.00 USD
  • Residual Protein A LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Residual Protein A Detection Kit is designed for detection and quantitation of Residual Protein A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • AlphaLisa Analyte 2

    Transthyretin (human), lyophilized (AlphaLISA)

    Recombinant human transthyretin (TTR) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human transthyretin detection kit, but can also be ordered separately.
    List Price : 759.00 USD
  • LANCE Detection Kit

    CHO HCP LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra CHO HCP Detection Kit is designed for detection and quantitation of CHO host cell proteins in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 830.00 USD - 11000.00 USD
  • LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence: CAGAGAIETDKEYYTVKD
    Price Range : 408.00 USD - 1250.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-EGF Receptor (Tyr1068) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-EGF Receptor (Tyr1068) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • LANCE_Fc_receptor

    FCGR3A/CD16a (176Val/V158) LANCE Ultra TR-FRET Binding Kit

    This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Val/V158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
    Price Range : 806.00 USD - 10700.00 USD
  • AlphaLISA immunoassay kits

    IgG1 (mouse) isotyping AlphaLISA Detection Kit

    This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.
    Price Range : 725.00 USD - 10100.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total BTK Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 907.00 USD - 52700.00 USD
  • IL-4 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 4 (IL-4) enables the quantitative determination of porcine IL-4 in cell culture media and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 15500.00 USD
  • AlphaLisa Analyte 2

    tPA (human), lyophilized (AlphaLISA)

    Human Tissue Plasminogen Activator (tPA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human tPA kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA immunoassay kits

    MCP2 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse MCP2 enables the quantitative determination of mouse monocyte chemoattractant protein 2 (MCP2; CCL8) in buffer and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1166.00 USD - 11100.00 USD
  • LANCE Ultra ULight™-poly GAT

    Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. Poly Glu-Ala-Tyr (1:1:1) labeled with ULight dye. This random polymer is a Tyrosine kinase substrate.
    Price Range : 436.00 USD - 999.00 USD
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