PerkinElmer

Drug Discovery Screening Reagents

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Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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1-25 of 1508 Products & Services

  • Frataxin (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • LANCE Detection Kit

    IL-6 (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • AlphaLISA Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
    Price Range : 1673.00 USD - 30500.00 USD
  • Cry1F AlphaLISA Detection Kit

    The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 715.00 USD - 11100.00 USD
  • Europium-labeled antibody for LANCE assays

    LANCE Eu-W1024 Anti-c-Myc

    Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
    Price Range : 324.00 USD - 15600.00 USD
  • AlphaLisa Analyte 2

    IL-17A (porcine), lyophilized (AlphaLISA)

    Porcine interleukin 17A (IL-17A) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IL-17A Detection Kit, but can also be ordered separately.
    List Price : 841.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
    Price Range : 395.00 USD - 26200.00 USD
  • AlphaLISA immunoassay kits

    IL-1β (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 1β (IL-1β) enables the quantitative determination of porcine IL-1β in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1268.00 USD - 12300.00 USD
  • AlphaLisa Analyte 2

    Hepatitis B Virus e Antigen (HBeAg), lyophilized (AlphaLISA)

    Hepatitis B virus e antigen (HBeAg) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA HBeAg Detection Kit, but can also be ordered separately.
    List Price : 481.00 USD
  • EGFR (human) LANCE Ultra TR-FRET Detection Kit

    The LANCE® Ultra Human EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
    Price Range : 865.00 USD - 11500.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra Total SMAD3 Assay Kit

    The AlphaLISA® SureFire® Ultra total SMAD3 assay is a sandwich immunoassay for quantitative detection of SMAD3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.
    Price Range : 526.00 USD - 34900.00 USD
  • AlphaPlex-545-antibody-bead2

    Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads

    AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.
    Price Range : 1692.00 USD - 30800.00 USD
  • Anti-rabbit IgG (Fc specific) AlphaLISA Acceptor Beads

    AlphaLISA® Acceptor beads conjugated to anti-rabbit IgG antibody. These beads can be used to capture rabbit IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
    Price Range : 700.00 USD - 28700.00 USD
  • alphascreen surefire total

    AlphaScreen SureFire Total IkB alpha Assay Kit

    The AlphaScreen® SureFire® total IκBα assay is a sandwich immunoassay for quantitative detection of IκBα (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • PerkinElmer

    Alpha SureFire Ultra Activation Buffer B

    Additional buffer for Alpha SureFire Ultra assays.
    Price Range : 126.00 USD - 664.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total p53 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-p53 (Ser15) + Total p53 assay kit is used to measure both the phosphorylation (on Ser15) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaLisa Analyte 2

    Beef troponin, liquid (AlphaLISA)

    Beef troponin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Beef Troponin Detection Kit, but can also be ordered separately.
    List Price : 278.00 USD
  • SureFire principle

    AlphaScreen SureFire JNK 123 (p-Thr183;Tyr185) Assay Kit

    The AlphaScreen® SureFire® p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1788.00 USD - 19100.00 USD
  • AlphaLISA Lysis Buffer

    Immunoassay buffer

    Pre-formulated buffer optimal for most AlphaLISA analyte detection assays. This buffer is used in most AlphaLISA immunoassay kits, and can be purchased to develop your own AlphaLISA immunoassay.
    Price Range : 240.00 USD - 458.00 USD
  • AlphaLisa Analyte 2

    IL-1α, lyophilized (AlphaLISA)

    Human Interleukin 1 alpha (IL1α) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IL1α kit, but can be ordered separately.
    List Price : 294.00 USD
  • AlphaScreen® P-Tyr-100 (Phosphotyrosine) Assay Kit

    AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine P-Tyr-100 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
    Price Range : 214.00 USD - 16400.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

    Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
    Price Range : 303.00 USD - 3531.00 USD
  • Testosterone AlphaLISA Detection Kit

    The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 768.00 USD - 12000.00 USD
  • Alpha donor antibody coated

    Anti-Human IgG Alpha Donor beads

    Alpha Donor beads conjugated to mouse anti-human IgG (Fc-specific) antibody. This bead can be used to capture human IgG antibodies containing an Fc-region for Alpha no-wash protein detection, Fc-receptor binding, antibody detection, antibody binding, and other applications.
    Price Range : 581.00 USD - 10500.00 USD
  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 (Tb) Human IFN-γ Detection Kit

    The AlphaPlex™ 545 (Terbium) human interferon-gamma (IFN-γ) Detection Kit is designed for the quantitative determination of human IFN-γ together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 549.00 USD - 12000.00 USD
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