PerkinElmer

DELFIA TRF Assay

OF

DELFIA® (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) TRF technology combines the benefits of Time Resolved Fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DEFLIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays.  By combining the benefits of TRF with the use of lanthanide chelates, DELFIA offers multiple advantages over standard fluorometric detection:

  • High sensitivity (femtogram level detection)
  • Wide dynamic range (4-5 log)
  • High S:B
  • Large Stokes shift
  • Superior stability – (days)
  • Multiplexing capability

From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.

 

DELFIAassay1
DELFIAassay1

Immunoassays

DELFIA delivers lower detection limits in the femtogram range, sensitivities that are at least 10x greater than standard colorimetric ELISA. DELFIA also offers a broad dynamic range, minimizing the need for time-consuming sample preparation and assay repeats.

Because DELFIA is not an enzyme-based technology, assay performance is not susceptible to degradation of enzyme conjugate activity or substrate signal. Lanthanide fluorescent signals are strong and stable, providing consistent signal measurement even months after an assay has been completed.

Furthermore, DELFIA maintains the wash steps of standard ELISA and so it is ideal when working in complex matrices.

Tcells
Tcells

Non-Radioactive Cytotoxicity and Cell Proliferation Assays

 

DELFIA cytotoxicity and cell proliferation assays provide an alternative to traditional radioactivity-based assays without compromising sensitivity or reliability.

The DELFIA Cytotoxicity assay closely mimics the traditional 51Cr-release protocol while the DELFIA Cell Proliferation assay couples 5-bromo-2-deoxyuridine (BrdU) incorporation with highly sensitive Europium-based TRF detection.

  • Sensitive
  • Time-saving
  • Convenient
  • Non-radioactive
BindingAssay
BindingAssay

Binding Assays

DELFIA provides a non-radioactive alternative to measure ligand-binding assays with Europium-labeled ligands, or biotinylated ligands in conjunction with Europium-streptavidin, using whole cells in adherent or filtration formats. It provides the sensitivity of traditional radioactive assays without the risk of exposure. DELFIA also offers a flexible, highly sensitive wash-based assay for protein-protein and other binding interactions that greatly reduces the potential for compound interference.

EnzymaticAssays
EnzymaticAssays

Enzymatic Assays

Enzymes are important targets in drug discovery. Many marketed drugs today function through inhibition of enzymes mediating disease phenotypes. DELFIA provides a time-resolved fluorescence enzymatic assay compatible with peptide, protein, histone, nucleosome, and other substrates for biochemical assays, or fixed and live cells for cell-based assays.  Furthermore, using DELFIA, multiplexing is possible.

OnPoint_2
OnPoint_2

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can conjugate your antibody or biomolecule to any of our DELFIA TRF chelates. Or maybe you are you short on time and need an assay developed quickly? Custom DELFIA assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your antibody or biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

 

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed.  Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development.  They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am?  We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website http://www.perkinelmer.com/DELFIA/ASK

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