PerkinElmer

DELFIA TRF Assay

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DELFIA® (Dissociation-Enhanced Lanthanide Fluorescent Immunoassay) TRF technology combines the benefits of Time Resolved Fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DEFLIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.

The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays.  By combining the benefits of TRF with the use of lanthanide chelates, DELFIA offers multiple advantages over standard fluorometric detection:

  • High sensitivity (femtogram level detection)
  • Wide dynamic range (4-5 log)
  • High S:B
  • Large Stokes shift
  • Superior stability – (days)
  • Multiplexing capability

From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.

DELFIAassay1
DELFIAassay1

Immunoassays

DELFIA delivers lower detection limits in the femtogram range, sensitivities that are at least 10x greater than standard colorimetric ELISA. DELFIA also offers a broad dynamic range, minimizing the need for time-consuming sample preparation and assay repeats.

Because DELFIA is not an enzyme-based technology, assay performance is not susceptible to degradation of enzyme conjugate activity or substrate signal. Lanthanide fluorescent signals are strong and stable, providing consistent signal measurement even months after an assay has been completed.

Furthermore, DELFIA maintains the wash steps of standard ELISA and so it is ideal when working in complex matrices.

Tcells
Tcells

Non-Radioactive Cytotoxicity and Cell Proliferation Assays

 

DELFIA cytotoxicity and cell proliferation assays provide an alternative to traditional radioactivity-based assays without compromising sensitivity or reliability.

The DELFIA Cytotoxicity assay closely mimics the traditional 51Cr-release protocol while the DELFIA Cell Proliferation assay couples 5-bromo-2-deoxyuridine (BrdU) incorporation with highly sensitive Europium-based TRF detection.

  • Sensitive
  • Time-saving
  • Convenient
  • Non-radioactive
BindingAssay
BindingAssay

Binding Assays

DELFIA provides a non-radioactive alternative to measure ligand-binding assays with Europium-labeled ligands, or biotinylated ligands in conjunction with Europium-streptavidin, using whole cells in adherent or filtration formats. It provides the sensitivity of traditional radioactive assays without the risk of exposure. DELFIA also offers a flexible, highly sensitive wash-based assay for protein-protein and other binding interactions that greatly reduces the potential for compound interference.

EnzymaticAssays
EnzymaticAssays

Enzymatic Assays

Enzymes are important targets in drug discovery. Many marketed drugs today function through inhibition of enzymes mediating disease phenotypes. DELFIA provides a time-resolved fluorescence enzymatic assay compatible with peptide, protein, histone, nucleosome, and other substrates for biochemical assays, or fixed and live cells for cell-based assays.  Furthermore, using DELFIA, multiplexing is possible.

OnPoint_2
OnPoint_2

OnPoint

Are you working on a novel target? If you are looking to develop your own assay PerkinElmer custom labeling services can conjugate your antibody or biomolecule to any of our DELFIA TRF chelates. Or maybe you are you short on time and need an assay developed quickly? Custom DELFIA assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your antibody or biomolecule custom-labeled or in developing a custom assay, please contact our OnPoint custom team.

iStock
iStock

How can we help you?

 

When it comes to developing assays or troubleshooting issues, always know you’re never on your own. With over 30 years’ experience in defining, synthesizing and optimizing chelate and chelate-based bioanalytical assays PerkinElmer is here to help you succeed.  Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.

FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development.  They are available to assist in both assay development and troubleshooting.

Application Support Knowledgebase (ASK): Need help at 1am?  We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more. Accessible 24 hours a day, 7 days a week on our website http://www.perkinelmer.com/DELFIA/ASK

Products & Services (56)
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1-25 of 56 Products & Services

  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

    Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
    Price Range : 252.00 USD - 3374.00 USD
  • Assay schematic for DELFIA Eu-streptavidin toolbox kit

    DELFIA Eu-labeled Goat Anti-HRP Toolbox Kit for ELISA Conversion

    DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled goat anti-HRP antibody for detection.
    Price Range : 560.00 USD - 866.00 USD
  • Assay schematic for DELFIA Eu-streptavidin toolbox kit
    New

    DELFIA Eu-labeled Streptavidin Toolbox Kit for ELISA Conversion

    DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled streptavidin for detection.
    Price Range : 134.00 USD - 655.00 USD
  • PerkinElmer

    DELFIA® Lysis Buffer, 30mL

    DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.
    List Price : 266.00 USD
  • Assay schematic for DELFIA Eu-streptavidin toolbox kit
    New

    DELFIA Eu-labeled Anti-Goat IgG Toolbox Kit for ELISA Conversion

    DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled anti-goat IgG antibody for detection.
    Price Range : 450.00 USD - 800.00 USD
  • DELFIA® Enhancer, 50mL

    DELFIA Enhancer solution for forming fluorescent Terbium and Dysprosium chelates in DELFIA time-resolved fluorescence (TRF) assays.
    List Price : 188.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
    New

    DELFIA Eu-N1 Mouse Anti-Goat IgG

    Europium-labeled anti-goat IgG antibody, for use in DELFIA® TRF assays
    Price Range : 289.00 USD - 3432.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-HA

    Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
    Price Range : 655.00 USD - 6300.00 USD
  • PerkinElmer

    DELFIA® Enhancement Solution, 1000 mL

    DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
    List Price : 883.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

    Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
    List Price : 1428.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA® Eu-N1 Anti-6xHis

    Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
    Price Range : 485.00 USD - 4523.00 USD
  • DELFIA Eu-N1-amino Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA®; Eu-N1 Goat anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
    Price Range : 758.00 USD - 3443.00 USD
  • DELFIA Eu-N1-DTA Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
    List Price : 2229.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Eu-N1 Goat Anti-HRP

    Europium-labeled anti-HRP IgG antibody, for use in DELFIA® TRF assays.
    Price Range : 145.00 USD - 379.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
    New

    DELFIA Eu-N1 Donkey Anti-Rabbit IgG

    Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
    Price Range : 290.00 USD - 3433.00 USD
  • PerkinElmer

    DELFIA® Wash Concentrate, 250mL

    Concentrated washing solution for DELFIA® time-resolved fluorescence (TRF) assays.
    List Price : 80.00 USD
  • DELFIA® Enhancement Solution, 250 mL

    DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
    List Price : 236.00 USD
  • PerkinElmer

    DELFIA® L*R wash concentrate (25x), 250 mL

    Wash buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
    List Price : 135.00 USD
  • DELFIA Assay Buffer

    DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
    Price Range : 40.00 USD - 722.00 USD
  • Assay schematic for DELFIA Eu-antibody toolbox kit
    New

    DELFIA Eu-labeled Anti-Mouse IgG Toolbox Kit for ELISA Conversion

    DELFIA® Toolbox kits allow you to build your own DELFIA time-resolved fluorescence (TRF) assay using your preferred antibody against the analyte of interest. Painlessly switch your traditional ELISA to one with a wider dynamic range, higher sensitivity, and great signal stability. This kit contains all necessary reagents to perform a sandwiching immunoassay using DELFIA Europium-labeled anti-mouse IgG antibody for detection.
    Price Range : 450.00 USD - 800.00 USD
  • DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

    DELFIA Tb-N1 Streptavidin

    Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
    Price Range : 320.00 USD - 2180.00 USD
  • DELFIA Eu-N1-Iodoacetomido Chelate and Europium (Eu) Standard, 1mg

    Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
    List Price : 2229.00 USD
  • DELFIA cell cytotox kit

    DELFIA® EuTDA Cytotoxicity Reagents

    DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.
    List Price : 424.00 USD
  • PerkinElmer

    DELFIA® Inducer, 250 mL

    DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.
    List Price : 246.00 USD
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