When washing and separation are essential to avoid high sample-dependent background or dissociate sticky complexes, turn to our DELFIA® time-resolved fluorescence assay for a solid-phase heterogeneous assay similar to ELISA – but with larger dynamic range and higher signal-to-noise. DELFIA utilizes the unique chemical properties of lanthanide chelates together with time-resolved fluorescence (TRF) detection to create a wash-based solid-phase assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility.
Advantages to our DELFIA TRF assay:
- High sensitivity: femtogram-level detection
- Wide dynamic range: 4–5 log
- Low background: large Stokes' shift and time-resolved measurement
- Superior stability: lanthanide chelates allow for delayed detection and batch processing
- Excellent flexibility: easily converted from 96-well to 384-well format
- Multiplexing capability
DELFIA TRF technology is used in a wide range of applications including:
- Immunodetection and immunogenicity
- Receptor-ligand binding
- Enzymatic assays
- Cell cytotoxicity and proliferation
- Protein-protein interactions
- Biodistribution
