PerkinElmer

DELFIA TRF Assay

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When washing and separation are essential to avoid high sample-dependent background or dissociate sticky complexes, turn to our DELFIA® time-resolved fluorescence assay for a solid-phase heterogeneous assay similar to ELISA – but with larger dynamic range and higher signal-to-noise. DELFIA utilizes the unique chemical properties of lanthanide chelates together with time-resolved fluorescence (TRF) detection to create a wash-based solid-phase assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility.

Advantages to our DELFIA TRF assay:

  • High sensitivity: femtogram-level detection
  • Wide dynamic range: 4–5 log
  • Low background: large Stokes' shift and time-resolved measurement
  • Superior stability: lanthanide chelates allow for delayed detection and batch processing
  • Excellent flexibility: easily converted from 96-well to 384-well format
  • Multiplexing capability

DELFIA TRF technology is used in a wide range of applications including:

  • Immunodetection and immunogenicity
  • Receptor-ligand binding
  • Enzymatic assays
  • Cell cytotoxicity and proliferation
  • Protein-protein interactions
  • Biodistribution

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