PerkinElmer

DELFIA TRF Assay

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When washing and separation are essential to avoid high sample-dependent background or dissociate sticky complexes, turn to our DELFIA® time-resolved fluorescence assay for a solid-phase heterogeneous assay similar to ELISA – but with larger dynamic range and higher signal-to-noise. DELFIA utilizes the unique chemical properties of lanthanide chelates together with time-resolved fluorescence (TRF) detection to create a wash-based solid-phase assay that offers high sensitivity, wide dynamic range, superior stability, and excellent flexibility.

Advantages to our DELFIA TRF assay:

  • High sensitivity: femtogram-level detection
  • Wide dynamic range: 4–5 log
  • Low background: large Stokes' shift and time-resolved measurement
  • Superior stability: lanthanide chelates allow for delayed detection and batch processing
  • Excellent flexibility: easily converted from 96-well to 384-well format
  • Multiplexing capability

DELFIA TRF technology is used in a wide range of applications including:

  • Immunodetection and immunogenicity
  • Receptor-ligand binding
  • Enzymatic assays
  • Cell cytotoxicity and proliferation
  • Protein-protein interactions
  • Biodistribution

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Application
  • Kinase (5)
  • Protein Analysis & Detection (23)
  • Protein-protein interactions (26)
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Molecular Modification
  • Phosphorylation (5)
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Antibody Conjugates
  • Anti-6X His (1)
  • Anti-c-Myc (1)
  • Anti-GST (1)
  • Anti-HA (1)
  • Anti-human IgG (1)
  • Anti-mouse IgG (1)
  • Anti-phospho-serine (1)
  • Anti-phospho-threonine (1)
  • Anti-rabbit IgG (1)
  • P-Tyr-100 (1)
  • PT66 (1)
  • PY20 (1)
  • Streptavidin (3)
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Fluorophore
  • Eu-DTPA-amino (1)
  • Eu-DTPA-DTA (1)
  • Eu-DTPA-ITC (1)
  • Eu-N1 (13)
  • Eu-N1-amino (1)
  • Eu-N1-DTA (1)
  • Eu-N1-Iodoacetamido (1)
  • Eu-N1-ITC (3)
  • Sm-N1 (1)
  • Sm-N1-ITC (1)
  • Tb-DTPA-ITC (1)
  • Tb-N1 (1)
  • Tb-N1-ITC (1)
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"DELFIA TRF Assay" 1-48 of 48 Products and Services

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DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Anti-phospho-(Ser) 14-3-3 motif, 10µg

AD0189
Europium-labeled anti-phospho-(Ser) 14-3-3 motif antibody, for capturing antibodies in DELFIA® TRF kinase assays.
List Price : 2837.00 USD
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DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

1244-302
DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 564.00 USD
PerkinElmer
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DELFIA® Research Buffer Set, 2x conc.

CR86-100
Protein- and detergent-free assay buffer in separate bottles, for DELFIA® time-resolved fluorescence (TRF) assays.
List Price : 389.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Anti-Phosphotyrosine (PY20)

AD0039 , AD0038
Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 339.00 USD - 3387.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 rabbit anti-mouse-IgG

AD0207 , AD0124
Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.
Price Range : 290.00 USD - 1256.00 USD
DELFIA cell cytotox kit
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DELFIA® EuTDA Cytotoxicity Reagents

AD0116
DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.
List Price : 424.00 USD
PerkinElmer
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DELFIA® Enhancement Solution, 1000 mL

4001-0010
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 883.00 USD
PerkinElmer
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DELFIA® 1 nmol/L Terbium standard solution, 50 mL

C558-100
For GLP or for general problem solving, lanthanide standard solutions are ready-to-use preparations of europium, samarium or terbium.
List Price : 128.00 USD
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DELFIA Eu-DTPA-DTA Chelate and Europium (Eu) Standard, 1mg

AD0024
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling. The DTPA chelate is more-stable than the N1 chelate.
List Price : 2229.00 USD
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DELFIA Eu-N1 ITC chelate & Eu standard, 1 mg

1244-301
DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 2229.00 USD
DELFIA BrdU cell proliferation kit
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DELFIA®Cell proliferation kit, 10 x 96 wells

AD0200
DELFIA® time-resolved fluorescence (TRF) cell proliferation kit for measuring cytotoxicity or proliferation in mammalian cells.
List Price : 743.00 USD
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DELFIA Eu-N1-amino Chelate and Europium (Eu) Standard, 1mg

AD0003
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
List Price : 2229.00 USD
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DELFIA Eu-N1-DTA Chelate and Europium (Eu) Standard, 1mg

AD0004
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The DTA-activated chelate reacts with free amino groups, sulfhydryl groups, and hydroxyl groups. Because of its high reactivity, we usually do not recommend DTA as it may “inactivate” the reagent you are labeling.
List Price : 2229.00 USD
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DELFIA® Assay Buffer without detergents, 5x conc.

CR85-100
Detergent-free assay buffer for DELFIA® time-resolved fluorescence (TRF) assays.
List Price : 888.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA® Eu-N1 Anti-HA

AD0053 , AD0054
Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
Price Range : 655.00 USD - 6300.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA® Eu-N1 Anti-c-myc

AD0112 , AD0113
Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in DELFIA® TRF assays.
Price Range : 700.00 USD - 6300.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA®; Eu-N1 Goat anti-Rabbit IgG

AD0105 , AD0106
Europium-labeled anti-rabbit IgG antibody, for use in DELFIA® TRF assays
Price Range : 758.00 USD - 3443.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Labeled Anti-Phosphothreonine Antibody, 10µg

AD0092
Europium-labeled anti-phosphothreonine antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays.
List Price : 1428.00 USD
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DELFIA Eu-DTPA-ITC Chelate and Europium (Eu) Standard, 1mg

AD0021
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
List Price : 2229.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA® Eu-N1 Anti-GST

AD0250 , AD0251
Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in DELFIA® TRF assays.
Price Range : 294.00 USD - 3387.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA® Eu-N1 Anti-6xHis

AD0108 , AD0109
Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
Price Range : 485.00 USD - 4523.00 USD
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DELFIA Eu-N1-Iodoacetomido Chelate and Europium (Eu) Standard, 1mg

AD0002
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The Iodacetamido-activated chelate reacts primarily with free sulfhydryl groups, such as cysteines.
List Price : 2229.00 USD
PerkinElmer
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DELFIA® Lysis Buffer, 30mL

4005-0010
DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.
List Price : 266.00 USD
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DELFIA Assay Buffer

1244-106 , 1244-111 , 4002-0010
DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
Price Range : 40.00 USD - 722.00 USD
PerkinElmer
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DELFIA® Inducer, 250 mL

4013-0010
DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.
List Price : 246.00 USD
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DELFIA Tb-N1-ITC Chelate and Terbium (Tb) Standard, 1mg

AD0009
Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
List Price : 2229.00 USD
PerkinElmer
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Stabilizer, 7.5% (DTPA-purified BSA), 50mL

CR84-100
BSA solution for DELFIA® time-resolved fluorescence (TRF) and LANCE® TR-FRET assays.
List Price : 371.00 USD
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DELFIA Sm-Labeling kit (Sm-N1-ITC Chelate), 0.2mg

1244-303
The DELFIA® Sm-Labeling kit is intended for labeling of proteins with samarium (Sm), for use in multiplexing dissociation enhanced time resolved fluoroimmunoassays.
List Price : 564.00 USD
PerkinElmer
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DELFIA 10 nmol/L Samarium standard solution, 50 mL

B115-100
Samarium standard solution for DELFIA time-resolved fluorescence (TRF) assays.
List Price : 131.00 USD
PerkinElmer
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DELFIA 1 nmol/L Europium standard solution, 50 mL

B119-100
Europium standard solution for DELFIA time-resolved fluorescence (TRF) assays.
List Price : 165.00 USD
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DELFIA Eu-N1-ITC Chelate and Eu Standard, 20 mg

AD0001
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts primarily with free amino groups, such as lysines.
List Price : 12200.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

AD0040 , AD0041
Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
Price Range : 291.00 USD - 3387.00 USD
PerkinElmer
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DELFIA® Wash Concentrate, 250mL

1244-114
Concentrated washing solution for DELFIA® time-resolved fluorescence (TRF) assays.
List Price : 80.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Tb-N1 Streptavidin

AD0048 , AD0047
Terbium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
Price Range : 320.00 USD - 2180.00 USD
PerkinElmer
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Fluorescein Standard Solution, 50mL, 100nMole/L

C557-100
100 nmol/L Fluorescein standard solution, 50 mL Shipped on blue ice.
List Price : 163.00 USD
PerkinElmer
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DELFIA® L*R binding buffer concentrate (10x), 250 mL

CR134-250
Buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
List Price : 571.00 USD
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DELFIA Tb-DTPA-ITC Chelate and Terbium (Tb) Standard, 1mg

AD0029
Terbium labeling chelate for creating your own Terbium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.
List Price : 2229.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Sm-N1 Streptavidin

AD0050 , AD0049
Samarium-labeled streptavidin for binding biotinylated biomolecules in DELFIA® time-resolved fluorescence (TRF) assays.
Price Range : 320.00 USD - 2180.00 USD
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DELFIA® Enhancer, 50mL

C500-100
DELFIA Enhancer solution for forming fluorescent Terbium and Dysprosium chelates in DELFIA time-resolved fluorescence (TRF) assays.
List Price : 188.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Anti-Human IgG, 100µg

1244-330
DELFIA® Europium-labeled anti-human IgG, for use in DELFIA time-resolved fluorescence (TRF) immunoassays.
List Price : 355.00 USD
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DELFIA Eu-DTPA-amino Chelate and Europium (Eu) Standard, 1mg

AD0023
Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups. The DTPA chelate is more-stable than the N1 chelate.
List Price : 2229.00 USD
PerkinElmer
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DELFIA® Enhancement Solution, 50 mL

1244-104
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 47.00 USD
PerkinElmer
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DELFIA® L*R wash concentrate (25x), 250 mL

CR135-250
Wash buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
List Price : 135.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

AD0160 , AD0159
Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 252.00 USD - 3374.00 USD
PerkinElmer
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DELFIA® BATDA Labeling Reagent, 50 µL

C136-100
BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.
List Price : 193.00 USD
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG
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DELFIA Eu-N1 Streptavidin

1244-360
DELFIA® Europium-labeled streptavidin, for use in DELFIA time-resolved fluorescence (TRF) assays.
List Price : 397.00 USD
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DELFIA® Enhancement Solution, 250 mL

1244-105
DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 236.00 USD
DELFIA Europium solution
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DELFIA® Eu-Solution, 200mL (for Cytotoxicity Detection Only)

C135-100
DELFIA® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.
List Price : 178.00 USD

"DELFIA TRF Assay" 1-48 of 48 Products and Services