PerkinElmer

Chemiluminescent Blotting Substrates

Lightning ONEChemiluminescent substrates are important tools in the detection of proteins and nucleic acids. Essentially, chemiluminescence blot detection starts when the primary antibody recognizes and binds to the target protein or nucleic acid immobilized on a membrane. This is followed by a secondary antibody labeled with an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). A chemiluminescent substrate for the particular enzyme is then applied to the blot causing a light emitting reaction which is then detected, signaling the presence of the target.

Lightning ONEWestern Lightning ONE, PerkinElmer’s premier line of premixed one-component chemiluminescent HRP substrates, are available with protein detection sensitivity from picogram to low femtogram to cover all your detection needs. Using a proprietary technology, our premixed solution enables greater experimental consistency by avoiding pipetting errors and possible contamination.

Western Lightning, our range of two-component chemiluminescent HRP substrates offers protein detection at varying sensitivities to fit your needs.

Western Lightning CDP-Star® chemiluminescent substrates are for the highly sensitive detection of AP antibody conjugated or streptavidin conjugated proteins.

DNA Thunder CDP-Star® chemiluminescent substrates are for the highly sensitive detection of AP antibody conjugated or streptavidin conjugated DNA.

 

Use our chemiluminescent substrates for sensitive and consistent detection of proteins or nucleic acids in a wide range of biochemistry, immunogenetic, and molecular biology applications.

Discover Our Chemiluminescent Blotting Substrates

Product Format Enzyme Blotting Method (Target) Recommended Membrane
Western Lightning ONE One bottle – premixed, no pipetting required HRP Western (protein) Protran nitrocellulose membrane
Western Lightning Two bottle – requires mixing HRP Western Protein Protran nitrocellulose membrane
Western Lightning CDP-Star® Ready to use - no diluting necessary AP Western Protein Protran nitrocellulose membrane
DNA Thunder CDP-Star® Ready to use - no diluting necessary AP Southern (DNA) GeneScreen nylon membrane

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