Chemiluminescent substrates are important tools in the detection of proteins and nucleic acids. Essentially, chemiluminescence blot detection starts when the primary antibody recognizes and binds to the target protein or nucleic acid immobilized on a membrane. This is followed by a secondary antibody labeled with an enzyme such as horseradish peroxidase (HRP) or alkaline phosphatase (AP). A chemiluminescent substrate for the particular enzyme is then applied to the blot causing a light emitting reaction which is then detected, signaling the presence of the target.
Western Lightning™, our range of two-component chemiluminescent HRP substrates offers protein detection at varying sensitivities to fit your needs.
Western Lightning™ CDP-Star® chemiluminescent substrates are for the highly sensitive detection of AP antibody conjugated or streptavidin conjugated proteins.
DNA Thunder™ CDP-Star® chemiluminescent substrates are for the highly sensitive detection of AP antibody conjugated or streptavidin conjugated DNA.
Use our chemiluminescent substrates for sensitive and consistent detection of proteins or nucleic acids in a wide range of biochemistry, immunogenetic, and molecular biology applications.
| Product | Format | Enzyme | Blotting Method (Target) | Recommended Membrane |
|---|---|---|---|---|
| Western Lightning™ | Two bottle – requires mixing | HRP | Western Protein | Protran™ nitrocellulose membrane |
| Western Lightning™ CDP-Star® | Ready to use - no diluting necessary | AP | Western Protein | Protran™ nitrocellulose membrane |
| DNA Thunder CDP-Star® | Ready to use - no diluting necessary | AP | Southern (DNA) | GeneScreen™ nylon membrane |
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