Discover Food Safety and Quality

AuroFlow PR1ME BT Combo MRL Assay

The AuroFlow™ PR1ME™ BT Combo MRL Assay is able to detect 14 beta-lactam antibiotics under the European MRL levels, as well as the metabolites of ceftiofur and cephaprin below MRL levels. The assay can detect all 3 regulated tetracycline antibiotics under the European MRL.The validation results indicate that the AuroFlow™ PR1ME™ BT Combo MRL Assay is appropriate for use as a screening test for beta-lactams and tetracyclines in raw commingled cow’s milk.

Validation results of the AuroFlow PRIME Beta-Lactam MRL Assay

AuroFlow™ PRIME Beta-Lactam MRL Assay (Bioo Scientific, Austin, TX) is a competitive receptor test in dipstick format for the rapid detection of residues of ?-lactams (penicillins and cefalosporins) in raw commingled cows’ milk. The time to result for the one-step assay is 3 minutes.

High Throughput Screening Solutions for Nitrofurans and Chloramphenicol in Shrimp Samples

The use of nitrofurans, a class of broad-spectrum antibiotics widely used in the aquaculture industry and their metabolites, has been regulated by everal countries and organizations across the world for their ahrmful effects on human health. ELISA assays are widely used for the detection of nitrofuran metabolites and chloramphenicol for regulatory conformance owing to the high sensitivity, selectivity and ease of use of the method. In this study, we demonstrate the accuracy and precision (CCß validation study) of the simultaneous 5-in-1 sample extraction method by manual ELISA and DS2 automation methods. Sensitivityof the assay kits (LOD) was also demonstrated using manual and DS2 method. Finally, sample variability testing was performed to characterize the effects of matrix from various shrimp sources.

Determination of the Nitrofuran Metabolites in Seafood using Ultra High-Performance Liquid Chromatography - Tandem Mass Spectrometry

Nitrofurans are a class of broad-spectrum antibiotics that are widely used to kill or slow down the growth of bacteria in the aquaculture industry. The use of nitrofurans and their metabolites has been restricted by a number of countries and organizations within the European Union, United States and China, due to their harmful side effects to human health, and a Minimum Required Performance Limit (MRPL) 0f 1.0 µg/kg has been set for food, animal and aquaculture products. LC/MS/MS methods have been used for the detection of nitrofuran metabolites in many studies owing to the high sensitivity, selectivity and specificity of the methods. However, nitrofuran metabolites demonstrate low ionization efficiency with the electrospray ion source used in LC/MS/MS systems. Therefore, the use of a derivatization step for nitrofuran metabolites before extraction and LC/MS/MS analysis is crucial to meet the low regulatory limits of 1.0 µg/kg in food samples. The main purpose of this work is to determine the detection limits for nitrofurans using QSight® 210 LC/MS/MS system.

Determination of Sulfonamides in Honey

In apiculture, sulfonamides are the most commonly used antibiotics to treat American and European Foulbrood, a type of disease that infects bees. This application note demonstrates how QSight 200 System can provides a very sensitive and robust platform for the analysis of sulfonamides in honey.

Determination, Confirmation and Quantitation of Multi-Class Antibiotic Residues in Milk by UHPLC MS/

A rapid, rugged and reliable LC/MS/MS method for the analysis of different antibiotic classes in milk with a simple sample preparation method showing good recoveries for most of the antibiotic classes. This method allowes the identification and quantification of target compounds in low ppb range (0.1 to 1 ppb) in milk with good precision and retention time stability.

A Multiclass Multiresidue Method for Analysis of Veterinary Drugs in Chicken by UHPLC/MS/MS

Veterinary drugs are used in animal production to treat diseases, prevent infection and protect growth of animals, which helps provide quality marketplace. However the uncontrolled use may cause heavy human disease so regulatory agencies around the world have established maximum residue levels (MRLs) or tolerances of veterinary drugs in foods. Drug analysis is generally challenging, due to the complexity of sample matrices and diversity of analytes from various classes of chemical properties. In this study, a fast, sensitive and selective method has been developed for analysis of 73 veterinary drugs (covering 13 different chemical classes) in chicken samples by coupling solvent extraction method with LC/MS/MS.

Determination of Thirteen Beta Agonists in Pork Using UHPLC - Tandem Mass Spectrometry

Beta-adrenergic receptor agonists (ß- agonists) are synthetic drugs utilized in the treatment of conditions such as asthma in humans, and are also used as a growth-promoting agent in food-producing animals. The use of ß- agonists in food-producing livestock has been either banned or regulated across many countries owing to human health concerns. To aid in the monitoring and detection of ß- agonists in livestock, a highly sensitive and selective analytical method has been developed, and is presented in this study. This LC/MS/MS method details the analysis of 13 ß- agonists in pork samples, and utilizes isotopically-labeled internal standards to compensate for matrix effects present in complex food matrices.