AlphaPlex Multiplexing Kits

AlphaPlex^™ is a homogeneous, all-in-one-well multiplexing reagent technology that provides highly sensitive detection of a wide range of analytes in a simple no-wash assay format based on our proven Alpha Technology. Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes.

• High sensitivity and accuracy
• High well-to-well reproducibility
• Wide range of analytes: from small nucleotides and large (MDa) proteins to scarce biological samples (primary and stem cells)
• Various affinities: from pM to mM (e.g. lectin-sugar interaction)
• Cost-savings from a 5-20 times reduction in sample consumption compared to other multiplexing platforms
• Simple, no-wash assay for easier set-up, saving you time and effort
• Use with Alpha-enabled filter-based readers such as the EnVision^® Multilabel Plate Reader, and JANUS^® Automated Workstation

The AlphaPlex assay technology enables easy, accurate multiplex quantification of a wide range of analytes, from large proteins to small proteins and scarce biological samples such as primary cells and stem cells, for increased predictivity and confidence in your results. It can be applied to a wide range of application including:

• Biomarkers for PD/PK
• Biomarkers for stem cells
• Kinase: total vs phosphorylated protein
• Epigenetic markers: total histone vs specific marker
• Amyloid peptides
• IgG profiling / testing
• Assay normalization with housekeeping proteins

AlphaPlex is a homogeneous luminescent proximity reagent technology that allows you to quantify multiple analytes in a single well. When the Donor and Acceptor beads are brought together, a cascade of chemical reactions is set in motion, causing a greatly amplified signal. By using multiple Acceptor beads which emit different wavelengths, multiple analytes can be detected.