PerkinElmer

AlphaLISA Biomarker Detection Kits

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Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. From assay development to primary and secondary screening, Alpha Technology provides researchers with robust and easy-to-use immunoassays adaptable to a variety of targets. With AlphaLISA, you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows for the detection of molecules of interest in buffer, cell culture media, serum, plasma and now urine in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Alpha Donor beads, while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules that triggers a cascade of energy transfer within the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. This light emission can then be detected on an Alpha-enabled reader.

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1-25 of 295 Products & Services

  • AlphaLISA immunoassay kits

    IL-12 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human interleukin-12 (IL-12; p70) enables the quantitative determination of human IL-12 in buffer, serum, plasma, and cell culture media using a homogeneous AlphaLISA assay (no wash steps). This kit replaces part numbers AL239C and AL239F.
    Price Range : 657.00 USD - 12000.00 USD
  • alphaimmunoassay1

    IL-17 (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin-17 (IL-17, IL17A) Detection Kit is designed for detection and quantitation of human IL-17 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    CXCL13 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CXCL13 enables the quantitative determination of human C-X-C Motif Chemokine Ligand 13 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12800.00 USD
  • AlphaLISA immunoassay kits

    TREM2 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human TREM2 enables the quantitative determination of human TREM2 in buffer, cell culture media, serum, plasma, cerebrospinal fluid and cell supernatant using a homogeneous AlphaLISA assay (no wash steps).
  • alphaimmunoassay1

    IL-1β (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interleukin-1β (IL1β) Detection Kit is designed for detection and quantitation of human IL-1β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1811.00 USD - 12000.00 USD
  • PerkinElmer

    BTK (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human Bruton's tyrosine kinase (BTK) enables the quantitative determination of human BTK in buffer and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLISA immunoassay kits

    TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 768.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    PDGF (BB dimer) (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human PDGF (BB homodimer) enables the quantitative determination of human platelet-derived growth factor (PDGF) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 715.00 USD - 17200.00 USD
  • AlphaLISA immunoassay kits

    HIV p24 AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 828.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    IRAK4 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human interleukin 1 receptor-associated kinase 4 (IRAK4) enables the quantitative determination of human IRAK4 in buffer, lysates, and tissue homogenates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 857.00 USD - 12600.00 USD
  • AlphaLISA immunoassay kits

    IL-4 (bovine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 531.00 USD - 11600.00 USD
  • AlphaLISA immunoassay kits

    Amyloid ß 1-x (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Amyloid beta 1-x (Aß 1-x) Detection Kit is designed for detection and quantitation of human Aß 1-x in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3470.00 USD - 27100.00 USD
  • AlphaLISA immunoassay kits

    IL-6 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 6 (IL-6) enables the quantitative determination of porcine IL-6 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1268.00 USD - 12300.00 USD
  • AlphaLISA immunoassay kits

    Albumin (human) AlphaLISA Detection Kit

    AlphaLISA no-wash immunoassay kit for detection of human serum albumin (HSA) in urine, buffered solution, or cell culture medium.
    Price Range : 3470.00 USD - 27100.00 USD
  • AlphaLISA immunoassay kits

    IgA (mouse) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 738.00 USD - 11500.00 USD
  • AlphaLISA immunoassay kits

    CD40 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLISA immunoassay kits

    IFN-β (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Interferon β (IFN-β) Detection Kit is designed for detection and quantitation of human IFN-β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-β 1a subtype.

    This product replaces AL265.

    Price Range : 1811.00 USD - 12000.00 USD
  • AlphaLISA immunoassay kits

    Galectin-9 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human galectin-9 enables the quantitative determination of human galectin-9 (Gal-9) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaLISA immunoassay kits

    PTEN (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human PTEN enables the detection and quantitation of human PTEN in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps). Other species of PTEN were not tested, but detection is expected with mouse and rat PTEN due to >99% sequence homology.
  • AlphaLISA immunoassay kits

    CCL1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CCL1 enables the quantitative determination of human chemokine (C-C motif) ligand 1 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 857.00 USD - 12100.00 USD
  • AlphaLISA immunoassay kits

    CD70 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD70 enables the quantitative determination of human CD70 in buffer, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 12600.00 USD
  • AlphaLISA immunoassay kits

    CD80 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaLISA immunoassay kits

    S100A4/FSP1 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human S100A4 enables the quantitative determination of human S100 calcium-binding protein A4 in serum, plasma, cerebrospinal fluid (CSF), buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • AlphaLISA immunoassay kits

    TNFα (bovine) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 768.00 USD - 12300.00 USD
  • AlphaLISA immunoassay kits

    BCL-2 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
    Price Range : 742.00 USD - 24100.00 USD
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