PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1033 Products & Services

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  • AlphaLISA immunoassay kits

    C-Peptide (mouse/rat) AlphaLISA Detection Kit

    The AlphaLISA® Mouse/Rat Connecting Peptide (C-Peptide) Detection Kit is designed for detection and quantitation of mouse or rat C-Peptide in buffered solution in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 3036.00 USD - 23700.00 USD
  • IgG4 (human) Pharmacokinetic AlphaLISA Detection Kit

    The AlphaLISA® Human IgG4 Pharmacokinetic Detection Kit is designed for the quantitative determination of human IgG4 in monkey serum and other samples, using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 1539.00 USD - 10200.00 USD
  • AlphaLisa Analyte 2

    Beef troponin, liquid (AlphaLISA)

    Beef troponin standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Beef Troponin Detection Kit, but can also be ordered separately.
    List Price : 243.00 USD
  • AlphaLisa Analyte 2

    PDGF (BB dimer) (human), lyophilized (AlphaLISA)

    Human PDGF (BB homodimer) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human PDGF Detection Kit, but can also be ordered separately.
    List Price : 308.00 USD
  • IL-1β (bovine) AlphaLISA Detection Kit

    The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1β in bovine serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 672.00 USD - 10700.00 USD
  • Alpha Fc receptor bind

    FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

    This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
    Price Range : 725.00 USD - 11100.00 USD
  • AlphaLisa Analyte 2

    S100A4/FSP1 (human), lyophilized (AlphaLISA)

    Human S100A4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human S100A4 Detection Kit, but can also be ordered separately.
    List Price : 243.00 USD
  • AlphaLISA immunoassay kits

    CTLA-4 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) enables the quantitative determination of human CTLA-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
  • AlphaLISA immunoassay kits

    GLP-1 (7-36) (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human GLP-1 enables the detection and quantitation of human GLP-1 (7-36 amide) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 725.00 USD - 10200.00 USD
  • SureFire principle

    AlphaScreen SureFire CREB (p-Ser133) Assay Kit

    The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • AlphaLISA immunoassay kits

    Soybean Agglutinin AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for Soybean Agglutinin enables the quantitative determination of Soybean agglutinin in food products in order to detect the presence of soybean proteins. It shows negligeable cross-reactivity with other lectins from alimentary plants.
    Price Range : 446.00 USD - 10500.00 USD
  • Alphaplex_545_protein_coated

    Streptavidin AlphaPlex™ 545 Acceptor Beads

    Streptavidin-coated AlphaPlex™ 545 (Terbium) Acceptor beads, for use in Alpha no-wash multiplexing assays.
    Price Range : 612.00 USD - 25100.00 USD
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