Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.
Choose the homogenous assay right for you:
AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.
Handle large biomolecular interactions with ease.
Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.
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Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire® CETSA® target engagement assays. For use in combination with Pelago Bioscience's CETSA® (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to CDK2, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA® subscription from Pelago Biosciences is required for use.
The AlphaLISA® SureFire® Ultra™ Human Phospho-ATM (Ser1981) assay is a sandwich immunoassay for quantitative detection of phospho-ATM (Ser1981) in cellular lysates using Alpha Technology.
Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire® CETSA® target engagement assays. For use in combination with Pelago Bioscience's CETSA® (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to CDK4, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA® subscription from Pelago Biosciences is required for use.
The AlphaLISA® SureFire® Ultra™ Human Phospho-GCN2 (Thr899) assay is a sandwich immunoassay for quantitative detection of phospho-GCN2 (Thr899) in cellular lysates using Alpha Technology.
The AlphaLISA® SureFire® Ultra™ Phospho-RSK1 (Ser380) assay is a sandwich immunoassay for quantitative detection of Phospho-RSK1 (Ser380) in cellular lysates using Alpha Technology.
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Phospho-ATM (Ser1981) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-ATM (Ser1981) assay kit, but can also be ordered separately.
The AlphaLISA® Mouse/Rat IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of mouse or rat IL-17 (IL-17A) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
This is a replacement for AL544.
Extra control lysate for the AlphaLISA® SureFire® Ultra™ Phospho-GCN2 (Thr899) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-GCN2 (Thr899) assay kit, but can also be ordered separately.
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