PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1073 Products & Services

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  • AlphaLisa Analyte 2

    PCSK9 (human), lyophilized (AlphaLISA)

    Human Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human PCSK9 kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA immunoassay kits

    Transthyretin (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human transthyretin enables the quantitative determination of human transthyretin (TTR) in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11100.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total p53 Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-p53 (Ser15) + Total p53 assay kit is used to measure both the phosphorylation (on Ser15) and total levels of endogenous p53 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure p53 phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 907.00 USD - 52700.00 USD
  • SureFire principle

    AlphaScreen SureFire STAT5 (p-Tyr694;Tyr699) Assay Kit 

    The AlphaScreen® SureFire® p-STAT5 (p-Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1703.00 USD - 18200.00 USD
  • TAU (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Microtubule-associated Protein Tau (TAU) Detection Kit is designed for detection and quantitation of human TAU in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies used in the kit correspond to clone numbers BT2 and Tau 12.
    Price Range : 3036.00 USD - 23700.00 USD
  • AlphaPlex-545-unconjugated

    Unconjugated AlphaPlex-545 Acceptor Beads

    Unconjugated AlphaPlex 545 (Terbium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
    Price Range : 1400.00 USD - 29500.00 USD
  • AlphaLisa Analyte 2

    EGFR (human), lyophilized (AlphaLISA)

    Human EGFR standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA EGFR kit, but can be ordered separately.
    List Price : 258.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) (Eu) + Total EGFR (Tb) Kit

    The Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) + Total EGFR assay kit is used to measure both phosphorylated and total levels of EGFR in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure EGFR (Tyr1068) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 907.00 USD - 52700.00 USD
  • AlphaLISA SureFire Ultra Lysate

    AlphaLISA SureFire Ultra Total SHP-2 Control Lysate

    Extra control lysate for the AlphaLISA SureFire Ultra total SHP-2 assay. This control is already provided in the AlphaLISA SureFire Ultra total SHP-2 assay kit, but can also be ordered separately.
    List Price : 22.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-4E-BP1 (Thr37/46) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-4E-BP1 (Thr37/46) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • Unconjugated AlphaLISA Acceptor beads

    Unconjugated AlphaLISA Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaLISA Acceptor beads for use in Alpha no-wash assays.
    Price Range : 1400.00 USD - 29400.00 USD
  • AlphaLisa Analyte 2

    Human Programmed Cell Death Protein 1 (PD-1), 0.3 µg (lyophilized)

    Human Programmed Cell Death Protein 1 (PD-1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human PD-1 Detection Kit, but can be ordered separately.
    List Price : 258.00 USD
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