PerkinElmer

Alpha Reagents

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Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-25 of 1090 Products & Services

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  • AlphaLISA immunoassay kits

    IL-6 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 6 (IL-6) enables the quantitative determination of porcine IL-6 in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • AlphaLisa Analyte 2

    IFNβ (porcine), lyophilized (AlphaLISA)

    Porcine interferon β (IFNβ) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IFNβ Detection Kit, but can also be ordered separately.
    List Price : 736.00 USD
  • AlphaLISA immunoassay kits

    TGF-β1 (human) AlphaLISA Biotin-Free Detection Kit

    The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 465.00 USD - 10200.00 USD
  • AlphaLISA immunoassay kits

    Transthyretin (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human transthyretin enables the quantitative determination of human transthyretin (TTR) in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11100.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-4E-BP1 (Thr37/46) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-4E-BP1 (Thr37/46) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • AlphaLISA immunoassay kits

    TNFα (cynomolgus) AlphaLISA Detection Kit

    The AlphaLISA® Cynomolgus TNFα Detection Kit is designed for detection and quantitation of cynomolgus and rhesus monkey tumor necrosis factor alpha in cell culture media using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 451.00 USD - 9900.00 USD
  • IFNβ (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interferon β (IFNβ) enables the quantitative determination of porcine IFNβ in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1109.00 USD - 10700.00 USD
  • PerkinElmer

    BTK (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human Bruton's tyrosine kinase (BTK) enables the quantitative determination of human BTK in buffer and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 11100.00 USD
  • AlphaLISA immunoassay kits

    IgG1 (mouse) isotyping AlphaLISA Detection Kit

    This AlphaLISA® immunoassay kit enables the isotyping of mouse IgG1 antibodies in cell culture media and non-mouse serum.
    Price Range : 725.00 USD - 10100.00 USD
  • AlphaLisa Analyte 2

    IFN-γ (ovine), lyophilized (AlphaLISA)

    Ovine IFNγ standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Ovine IFNγ Detection Kit, but can also be ordered separately.
    List Price : 243.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ Multiplex phospho/total BTK Assay Kit

    The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 907.00 USD - 52700.00 USD
  • IL-4 (porcine) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for porcine interleukin 4 (IL-4) enables the quantitative determination of porcine IL-4 in cell culture media and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 782.00 USD - 15500.00 USD
  • AlphaLISA immunoassay kits

    Tissue Plasminogen Activator (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Tissue Plasminogen Activator (tPA) Detection Kit is designed for detection and quantitation of human tPA in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both tPA and pro-tPA.
    Price Range : 1585.00 USD - 10500.00 USD
  • AlphaLISA immunoassay kits

    MCP2 (mouse) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for mouse MCP2 enables the quantitative determination of mouse monocyte chemoattractant protein 2 (MCP2; CCL8) in buffer and serum using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1166.00 USD - 11100.00 USD
  • alphascreen surefire total

    AlphaScreen SureFire Total Topo Iia Assay Kit

    The AlphaScreen® SureFire® total Topoisomerase II-α assay is a sandwich immunoassay for quantitative detection of Topoisomerase II-α (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • IL-1RA (human) AlphaLISA Detection Kit

    The AlphaLISA® Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 465.00 USD - 10200.00 USD
  • AlphaLISA immunoassay kits

    pNFH (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human phosphorylated neurofilament heavy chain protein (pNFH) enables the quantitative determination of human pNFH in plasma or cerebrospinal fluid (CSF) using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 750.00 USD - 11100.00 USD
  • AlphaLISA SureFire Ultra kits

    Total STAT3 AlphaLISA SureFire Ultra Assay Kit

    The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
    Price Range : 504.00 USD - 29200.00 USD
  • AlphaScreen® P-Tyr-100 (Phosphotyrosine) Assay Kit

    AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine P-Tyr-100 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
    Price Range : 188.00 USD - 14400.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire® Ultra™ phospho-AKT1 (Ser473) Assay Kit

    The AlphaLISA® SureFire® Ultra™ phospho-AKT1 (Ser473) assay kit can be used to measure levels of AKT1 phosphorylated at residue Ser473 in cellular lysates using no-wash Alpha technology.
    Price Range : 504.00 USD - 30400.00 USD
  • AlphaLISA SureFire Ultra kits

    AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

    The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 29200.00 USD
  • Alpha SureFire Ultra Multiplex kits

    Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

    The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 907.00 USD - 52700.00 USD
  • TAU (human) AlphaLISA Detection Kit

    The AlphaLISA® Human Microtubule-associated Protein Tau (TAU) Detection Kit is designed for detection and quantitation of human TAU in cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The antibodies used in the kit correspond to clone numbers BT2 and Tau 12.
    Price Range : 3036.00 USD - 23700.00 USD
  • AlphaLisa Analyte 2

    tPA (human), lyophilized (AlphaLISA)

    Human Tissue Plasminogen Activator (tPA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human tPA kit, but can be ordered separately.
    List Price : 257.00 USD
  • AlphaLISA SureFire Ultra kits

    Phospho-SHP-1 (Tyr564) AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ p-SHP-1 (Tyr564) assay is a sandwich immunoassay for quantitative detection of phospho-SHP-1 (phosphorylated on Tyr564) in cellular lysates using Alpha Technology.
    Price Range : 504.00 USD - 30400.00 USD
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