PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
  • Products and Services

  • Resources, Events & More

Narrow your results
Antibody Conjugates
  • Anti-6X His (3)
  • Anti-Acetyl-Lysine (1)
  • Anti-bovine IgA (1)
  • Anti-bovine IgG (1)
  • Anti-bovine IgG1 (1)
  • Anti-bovine IgG2 (1)
  • Anti-bovine IgM (1)
  • Anti-c-Myc (1)
  • Anti-C-tag (1)
  • Anti-chicken IGY (1)
  • Anti-DIG (2)
  • Anti-DNP (2)
  • Anti-FITC (1)
  • Anti-FLAG (4)
  • Anti-GFP (2)
  • Anti-goat IgG (1)
  • Anti-GST (1)
  • Anti-H3 (C-term) (1)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2-1 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (2)
  • Anti-H3K4 (2)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H3R2me (1)
  • Anti-H4K20me1 (1)
  • Anti-H4R3me (1)
  • Anti-HA (2)
  • Anti-HRP (2)
  • Anti-human IgE (1)
  • Anti-human IgG (3)
  • Anti-human IgG1 (2)
  • Anti-human IgG2 (1)
  • Anti-human IgG3 (1)
  • Anti-human IgG4 (2)
  • Anti-MBP (1)
  • Anti-methyl-Arg (1)
  • Anti-mouse IgA (1)
  • Anti-mouse IgE (1)
  • Anti-mouse IgG (5)
  • Anti-mouse IgG1 (1)
  • Anti-mouse IgG2a (1)
  • Anti-mouse IgG2b (1)
  • Anti-mouse IgG3 (1)
  • Anti-mouse IgM (2)
  • Anti-O-GlcNAc (1)
  • Anti-p53K382ac (1)
  • Anti-rabbit IgG (3)
  • Anti-rat IgG (2)
  • Anti-sheep IgG (1)
  • Anti-V5 (1)
  • CaptSure (1)
  • Glutathione (3)
  • Human Fc-fragment (2)
  • LCA (1)
  • Nickel chelate (2)
  • Protein A (5)
  • Protein G (1)
  • Protein L (1)
  • Strep-Tactin (2)
  • Streptavidin (4)
  • Streptavidin/Anti-c-Myc (1)
  • Streptavidin/Anti-DIG (1)
  • Streptavidin/Anti-FITC (1)
  • Streptavidin/Anti-FLAG (1)
  • Streptavidin/Anti-GST (1)
  • Streptavidin/Anti-HA (1)
  • Streptavidin/Anti-mouse IgG (1)
  • Streptavidin/Anti-rabbit IgG (1)
  • Streptavidin/Nickel chelate (1)
  • Streptavidin/P-Tyr-100 (1)
  • Streptavidin/Phosphosensor (1)
  • Streptavidin/Protein A (1)
  • Streptavidin/PT66 (1)
  • Streptavidin/PY20 (1)
  • Streptavidin/Unconjugated (1)
  • Unconjugated (5)
Application
  • Antibody binding (3)
  • cAMP (1)
  • Epigenetics (36)
  • High biotin samples (8)
  • Isotyping (12)
  • Kinase (159)
  • Multiplexing (45)
  • Protein Analysis & Detection (806)
  • Protein-nucleic acid interactions (4)
  • Protein-protein interactions (83)
Assay points(200-50,000)
  • 100 (274)
  • 1000 (6)
  • 10000 (153)
  • 500 (465)
  • 5000 (317)
  • 50000 (151)
Assay Target
  • 4EBP1 (5)
  • A1AT (2)
  • Acetyl CoA carboxylase (2)
  • Adiponectin (2)
  • Aflatoxin (2)
  • Agglutinin (soybean) (2)
  • Akt1 (5)
  • Akt1/2 (1)
  • Akt1/2/3 (8)
  • AKT1/2/3 (2)
  • Albumin (5)
  • ALK (3)
  • Alpha-synuclein (2)
  • AMPKα (2)
  • ANGPT1 (2)
  • ANGPT2 (2)
  • ANGPT3 (2)
  • ANGPTL4 (2)
  • ApoA1 (2)
  • ApoB (2)
  • ApoE (2)
  • AST (2)
  • ATF1 (1)
  • ATF2 (1)
  • Aβ1-40 (2)
  • Aβ1-42 (4)
  • Aβ1-x (1)
  • Aβ40 (4)
  • Aβ42 (2)
  • B7-H5 (2)
  • BACE-1 (2)
  • BAD (2)
  • BCL-2 (2)
  • BDNF (2)
  • Beef troponin (2)
  • Beta-2 microglobulin (2)
  • BMP-7 (2)
  • BMP-9 (2)
  • BRCA2 (2)
  • BTLA (2)
  • c-Jun (2)
  • c-Met (3)
  • C-peptide (4)
  • CA125 (2)
  • cAMP (1)
  • cardiac Troponin I (2)
  • Cardiac Troponin T (2)
  • Caspase-9 (1)
  • CCL1 (2)
  • CCL2 (4)
  • CCL3 (2)
  • CCL4 (2)
  • CCL5 (4)
  • CD117 (2)
  • CD276 (2)
  • CD28 (2)
  • CD80 (3)
  • CD86 (3)
  • Chk-1 (1)
  • Chk-2 (1)
  • Chloramphenicol (2)
  • CHO HCP (2)
  • CHO-P (2)
  • Claudin 7 (2)
  • Cofilin (6)
  • COL1A1 (1)
  • Collagen IV (2)
  • Corticosterone (2)
  • Cortisol (1)
  • COX-2 (2)
  • CREB (4)
  • CRP (2)
  • Cry1Ab/1Ac (2)
  • Cry1F (2)
  • Cry2A (2)
  • CTLA4 (4)
  • CXCL1/GROα (2)
  • CXCL10 (4)
  • CXCL11 (2)
  • CXCL12 (2)
  • CXCL13 (4)
  • CXCL4/PF4 (2)
  • CXCL9 (2)
  • Cyr61 (2)
  • D-dimer (2)
  • E-Cadherin (2)
  • EGF (3)
  • EGFR (8)
  • eIF2α (3)
  • eIF4E (4)
  • Elk1 (1)
  • EPO (2)
  • ErbB2 (4)
  • ERK1/2 (8)
  • Factor VIII (3)
  • FCGR1 (1)
  • FCGR2A (2)
  • FCGR2B (1)
  • FCGR3A (4)
  • FGF23 (2)
  • Fibrinogen (2)
  • Fibronectin (2)
  • FLT-1 (2)
  • Frataxin (2)
  • FSH (2)
  • FSP1 (2)
  • G-CSF (2)
  • GAD67 (2)
  • Galectin 9 (2)
  • GAPDH (4)
  • GDNF (2)
  • Ghrelin (4)
  • GITR (2)
  • GLP-1 (2)
  • Glucagon (2)
  • GM-CSF (4)
  • Growth Hormone (2)
  • GSK3α (1)
  • GSK3β (3)
  • HBeAg (2)
  • HBsAg (2)
  • hCG (2)
  • Histone H2A (1)
  • Histone H3 (10)
  • Histone H4 (3)
  • HLA-C (2)
  • HLA-DRA (1)
  • HLA-E (2)
  • HO-1 (2)
  • HO-2 (2)
  • Hsp27 (1)
  • HVEM (2)
  • Hyaluronic Acid (2)
  • ICAM-1 (4)
  • IFN-λ2 (2)
  • IFNα (6)
  • IFNβ (4)
  • IFNγ (15)
  • IgA (4)
  • IgE (2)
  • IGF1 (4)
  • IGF2 (2)
  • IGFR (3)
  • IgG (6)
  • IgG1 (6)
  • IgG2 (2)
  • IgG2a (2)
  • IgG2b (2)
  • IgG3 (2)
  • IgG4 (4)
  • IgM (6)
  • IKKα (5)
  • IL-1RA (2)
  • IL-23 (2)
  • IL-6 (3)
  • IL10 (8)
  • IL11 (2)
  • IL12 (8)
  • IL13 (8)
  • IL15 (1)
  • IL17 (1)
  • IL17A (14)
  • IL17F (2)
  • IL18 (6)
  • IL18BP (2)
  • IL1α (4)
  • IL1β (12)
  • IL2 (9)
  • IL21 (2)
  • IL22 (3)
  • IL23 (2)
  • IL25 (4)
  • IL28B (2)
  • IL31 (2)
  • IL33 (4)
  • IL4 (9)
  • IL5 (4)
  • IL6 (9)
  • IL7 (2)
  • IL8 (7)
  • Influenza A NP (1)
  • INSR (4)
  • Insulin (5)
  • IP10 (6)
  • IκBα (2)
  • JNK1/2/3 (4)
  • JNK1/3 (1)
  • Kappa light chain (2)
  • KIM-1 (2)
  • LAG3 (3)
  • Lambda light chain (2)
  • Laminin (2)
  • LC3B (2)
  • LDH (2)
  • Leptin (4)
  • MANF (2)
  • MBNL1 (2)
  • MEK1 (4)
  • Mesothelin (2)
  • MKK3/6 (1)
  • MKK4 (1)
  • MMP-12 (2)
  • MMP-7 (2)
  • MMP1 (2)
  • MMP13 (2)
  • MMP2 (2)
  • MMP3 (2)
  • MMP8 (2)
  • MMP9 (4)
  • mTOR (3)
  • Myeloperoxidase (2)
  • Myoglobin (2)
  • N-cadherin (2)
  • Neprilysin (2)
  • NFκB (7)
  • NGAL (2)
  • Nidogen-1 (2)
  • NNMT (2)
  • Oncostatin M (2)
  • Osteoprotegerin (2)
  • OX40L (2)
  • p24 (5)
  • p38 MAPK (7)
  • p53 (5)
  • p62 (4)
  • p70 S6K (7)
  • PAI-1 (2)
  • PCSK9 (2)
  • PD-1 (4)
  • PD-L1 (4)
  • PD-L2 (2)
  • PDGF (2)
  • PDGF-BB (2)
  • PDGFR (2)
  • PDK1 (1)
  • PEG IgM (1)
  • PER.C6 HCP (2)
  • Perlecan (2)
  • PIGF (2)
  • PIP Collagen (2)
  • PON1 (2)
  • PON2 (1)
  • Progesterone (2)
  • PSA (2)
  • PSMA (2)
  • PTEN (2)
  • Residual DNA (2)
  • Residual Protein A (2)
  • S100A4 (2)
  • S6RP (4)
  • SCF (2)
  • SDF1 (2)
  • SHP2 (2)
  • SLP-76 (3)
  • SMAD1 (4)
  • SMAD2 (1)
  • SMAD3 (4)
  • Somatostatin (2)
  • STAT1 (5)
  • STAT3 (5)
  • STAT4 (2)
  • STAT5 (5)
  • STAT6 (2)
  • SYK (3)
  • Tau (2)
  • TDP-43 (2)
  • Testosterone (2)
  • TFF3 (2)
  • TGF-β1 (3)
  • TIM-3 (2)
  • TIMP1 (2)
  • TIMP2 (2)
  • TNFR1 (4)
  • TNFα (13)
  • Topo IIα (3)
  • tPA (2)
  • TRAIL (2)
  • Transferrin (2)
  • TrkA (1)
  • TrkB (1)
  • TSH (2)
  • TSLP (4)
  • Utrophin (2)
  • VCAM-1 (2)
  • VEGF-A (2)
  • VEGF2 Receptor (5)
  • VEGFR1 (2)
  • VEGFR2 (1)
  • Vimentin (2)
  • VISTA (2)
  • α-tubulin (3)
  • β-Catenin (1)
  • β-NGF (2)
Assay Target Class
  • Aminoglycan (2)
  • Antibody (41)
  • Collagen (3)
  • Cytokine (215)
  • DNA (2)
  • Fc-receptor (8)
  • Histone (13)
  • Hormone (36)
  • Peptide (20)
  • Phosphoprotein (149)
  • Protein (322)
  • Small Molecule (2)
Automation Compatible
  • No (1)
  • Yes (960)
Detection Method
  • Alpha (926)
Experimental Type
  • In vitro (682)
Format
  • Microplates (732)
Molecular Modification
  • Acetylation (8)
  • Glycosidation (1)
  • Methylation (16)
  • Phosphorylation (65)
  • Total (1)
  • Unmodified (4)
Product Brand Name
  • Alpha (14)
  • Alpha SureFire Ultra Multiplex (34)
  • AlphaLISA (680)
  • AlphaLISA SureFire Ultra (77)
  • AlphaPlex (42)
  • AlphaScreen (22)
  • AlphaScreen SureFire (71)
  • LANCE Ultra (2)
Target Species
  • Bovine (37)
  • Cynomolgus (4)
  • Human (548)
  • Monkey (2)
  • Mouse (72)
  • Ovine (2)
  • Plant (6)
  • Porcine (16)
  • Rat (4)
Therapeutic Area
  • Agriculture/Plant (6)
  • Angiogenesis (16)
  • Autophagy (7)
  • Biologics/Bioprocess (68)
  • Cancer (153)
  • Cardiovascular (22)
  • Central Nervous System (33)
  • Epigenetics (13)
  • Food Testing (6)
  • Inflammation (203)
  • Kidney (2)
  • Metabolic (55)
  • Virology (6)

"Alpha Reagents" 1-50 of 973 Products and Services

Sort
Per Page

IL-4 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 446.00 USD - 9700.00 USD
Alphaplex-545-terbium-glutathione

Glutathione AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to glutathione, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-Human IgG AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-human IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD3 (Ser423/425) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD3 (Ser423/425) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human Insulin Detection Kit

The AlphaPlex™ 645 (Samarium) Human Insulin Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 461.00 USD - 10000.00 USD
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD

AlphaLISA Anti-mono-methyl-Histone H4 Lysine 20 Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against Histone H4 that is mono-methylated at lysine 20 (H4K20me1). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1420.00 USD - 25900.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human IL2 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 607.00 USD - 10000.00 USD
AlphaPlex Samarium Acceptor Beads

Anti-6X His AlphaPlex 645 (Sm) Acceptor beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
Alphaplex_545_protein_coated

Streptavidin AlphaPlex™ 545 Acceptor Beads

Streptavidin-coated AlphaPlex™ 545 (Terbium) Acceptor beads, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD

Anti-Sheep IgG AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-sheep IgG antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture sheep IgG antibodies for AlphaLISA no-wash assays.
Price Range : 588.00 USD - 24100.00 USD
SureFire principle

AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1653.00 USD - 17700.00 USD
AlphaLISA immunoassay kits

Mesothelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human mesothelin enables the quantitative determination of human mesothelin (CAK1, ERC) in serum, buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14400.00 USD
AlphaLISA immunoassay kits

Hepatitis B Virus e Antigen (HBeAg) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for hepatitis B virus (e antigen; HBeAg) enables the quantitative determination of HBeAg in cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 750.00 USD - 14800.00 USD
AlphaLisa Analyte 2

Claudin 7 (human), lyophilized (AlphaLISA)

Human claudin 7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Claudin 7 Detection Kit, but can be ordered separately.
List Price : 425.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL17 Detection Kit

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 359.00 USD - 10000.00 USD
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Thr1343) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLisa Analyte 2

IgM (mouse), lyophilized (AlphaLISA)

Mouse IgM lyophilized standard, for use in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse IgM isotyping kit, but can be ordered separately.
List Price : 238.00 USD
AlphaLISA immunoassay kits

Tissue Plasminogen Activator (human) AlphaLISA Detection Kit

The AlphaLISA® Human Tissue Plasminogen Activator (tPA) Detection Kit is designed for detection and quantitation of human tPA in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both tPA and pro-tPA.
Price Range : 1520.00 USD - 10100.00 USD

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 645.00 USD - 10100.00 USD
AlphaLisa Analyte 2

TFF3 (human), lyophilized (AlphaLISA)

Human Trefoil Factor 3 (TFF3) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human TFF3 kit, but can be ordered separately.
List Price : 247.00 USD
AlphaLISA immunoassay kits

IgM (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine IgM in serum, plasma, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 645.00 USD - 10300.00 USD

Anti-methyl-Histone H4 Arginine 3 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H4 methylated at arginine 3 (H4R3me). This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 1420.00 USD - 25900.00 USD
Alpha donor antibody coated

Anti-GFP Alpha Donor Beads

Alpha Donor beads conjugated to anti-GFP antibody. The antibody is a mouse monoclonal antibody that recognizes wildtype and recombinant GFP (rGFP), and cross-reacts with eGFP. These beads can be used to capture GFP-tagged proteins for AlphaScreen, AlphaLISA, and AlphaPlex no-wash assays.
Price Range : 612.00 USD - 8800.00 USD
AlphaPlex-545-antibody-bead2

Anti-6X His AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 587.00 USD - 24100.00 USD
AlphaLISA Buffer

AlphaLISA universal buffer

Pre-formulated buffer used to QC Alpha assays. This buffer can sometimes be used for protein-protein binding, proof-of-concept, and other Alpha assays.
Price Range : 202.00 USD - 384.00 USD
PerkinElmer

AlphaLISA SureFire Ultra Phospho-Acetyl CoA Carboxylase (Ser79) Lysate

Extra control lysate for the AlphaLISA SureFire Ultra phospho-acetyl CoA carboxylase (Ser79) assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-ACC assay kit, but can also be ordered separately.
List Price : 21.00 USD
Alpha_Strep-Tactin_Donor_bead

Strep-Tactin® Alpha Donor beads

Alpha Donor beads conjugated to Strep-Tactin®. These beads can be used to capture Strep-tag® II-tagged proteins for Alpha no-wash assays.
Price Range : 481.00 USD - 8700.00 USD

Aflatoxin AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for aflatoxin enables the quantitative determination of aflatoxin in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 478.00 USD - 9800.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 489.00 USD - 28400.00 USD

IgG (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgG in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 695.00 USD - 9700.00 USD
AlphaLISA immunoassay kits

LC3B (human) AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of human LC3B in buffered solution or cell lysates. The antibodies in the kit detect both LC3B type I and type II. The analyte in this kit consists of recombinant LC3B fused to His-tag at the N-terminus.
Price Range : 2827.00 USD - 22100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-p70 S6 Kinase (Thr389) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
PerkinElmer

Alpha SureFire Ultra Dilution Buffer

Additional buffer for Alpha SureFire Ultra assays.
Price Range : 118.00 USD - 618.00 USD
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.
Price Range : 695.00 USD - 10100.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SMAD1 (Ser463/465) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SMAD1 (Ser463/465) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Price Range : 478.00 USD - 9800.00 USD
AlphaLISA immunoassay kits

IL-6 (bovine) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for quantitative detection of bovine IL-6. This kit permits detection of bovine IL-6 in bovine serum, plasma, and cell culture supernatants.
Price Range : 696.00 USD - 10300.00 USD
AlphaLISA immunoassay kits

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 600.00 USD - 14400.00 USD
AlphaPlex Terbium immunoassay kits

AlphaPlex™ 545 Human Insulin Detection Kit

The AlphaPlex™ 545 (Terbium) Human Insulin Detection Kit is designed for the quantitative determination of human Insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 461.00 USD - 10000.00 USD
AlphaLISA immunoassay kits

IFN-γ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine IFN-γ enables the detection and quantitation of porcine interferon-gamma in serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human, bovine, ovine, or equine IFNγ. Cross reactivity with other species has not been tested.
Price Range : 622.00 USD - 20200.00 USD
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1493.00 USD - 16100.00 USD
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 489.00 USD - 28400.00 USD
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-MEK1 (Ser218/222) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-MEK1 (Ser218/222) in a multiplexed assay.
Price Range : 367.00 USD - 22100.00 USD
AlphaPlex Samarium Acceptor Beads

Streptavidin AlphaPlex™ 645 (Sm) Acceptor Beads

The Streptavidin coated AlphaPlex™ 645 (Samarium) Acceptor beads are used to capture a wide variety of biotinylated reagents, ranging from small nucleotides to very large proteins. To avoid steric hindrance, it is recommended to use biotin derivatives including an aliphatic spacer composed of at least six carbons.
Price Range : 587.00 USD - 24100.00 USD

Anti-Chicken IgY AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-chicken IgY antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture chicken IgY antibodies for AlphaLISA no-wash assays.
Price Range : 588.00 USD - 24100.00 USD

TNFα (mouse) AlphaLISA Detection Kit

The AlphaLISA Mouse TNFα Detection Kit is designed for the quantitative determination of mouse TNFα in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 1520.00 USD - 10100.00 USD

"Alpha Reagents" 1-50 of 973 Products and Services

Per Page