PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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1-12 of 1064 Products & Services

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  • AlphaPlex Terbium immunoassay kits

    AlphaPlex-545 Human IL1β Detection Kit

    The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
    Price Range : 613.00 USD - 10500.00 USD
  • AlphaLISA SureFire Ultra kits

    Total p70 S6K AlphaLISA SureFire Ultra Detection Kit

    The AlphaLISA® SureFire® Ultra™ total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-p70 S6K assay.
    Price Range : 504.00 USD - 30400.00 USD
  • AlphaLISA immunoassay kits

    CCL4/MIP-1ß (human) AlphaLISA Detection Kit

    The AlphaLISA® Human C-C Motif Chemokine 4 / Macrophage Inflammatory Protein-1beta (CCL4 / MIP-1ß) Detection Kit is designed for detection and quantitation of human CCL4/MIP-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
    Price Range : 1693.00 USD - 10500.00 USD
  • Assay principle of Alpha Terbium SureFire Ultra assay

    Phospho-ERK1/2 (Thr202/Tyr204) Terbium SureFire Ultra Detection Kit

    The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-ERK1/2 (Thr202/Tyr204) in a multiplexed assay.
    Price Range : 378.00 USD - 22800.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

    The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1687.00 USD - 18200.00 USD
  • GLP-1 (7-36) (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human GLP-1 enables the detection and quantitation of human GLP-1 (7-36 amide) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 725.00 USD - 10200.00 USD
  • Alpha donor antibody coated

    Anti-Human IgG Alpha Donor beads

    Alpha Donor beads conjugated to mouse anti-human IgG (Fc-specific) antibody. This bead can be used to capture human IgG antibodies containing an Fc-region for Alpha no-wash protein detection, Fc-receptor binding, antibody detection, antibody binding, and other applications.
    Price Range : 509.00 USD - 9200.00 USD
  • SureFire principle

    AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

    The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1538.00 USD - 16600.00 USD
  • AlphaLisa Analyte 2

    IL-17A (mouse), lyophilized (AlphaLISA)

    Mouse IL-17A standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA mouse IL-17A kits, but can also be ordered separately.
    List Price : 257.00 USD
  • AlphaPlex Samarium immunoassay kits

    AlphaPlex-645 (Sm) Human TNFα Detection Kit

    The AlphaPlex™ 645 (Samarium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
    Price Range : 633.00 USD - 10500.00 USD
  • Anti-HA AlphaLISA Acceptor Beads

    AlphaLISA Acceptor beads conjugated to anti-HA (hemagglutinin) antibody. These beads can be used to capture HA-tagged proteins and peptides.
    Price Range : 613.00 USD - 25200.00 USD
  • AlphaLISA immunoassay kits

    CD28 (human) AlphaLISA Detection Kit

    The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 625.00 USD - 15000.00 USD
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