Alpha Kits

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Alpha kits are ready to use for quick optimization and execution of assays for a variety of targets. Alpha technology, an alternative to a traditional ELISA assay kit, enables the detection and screening of molecules-of-interest in a no-wash, highly sensitive, quantitative assay.

Cytokine and biomarker detection: Avoid a troublesome, traditional ELISA reagent kit with a far superior alternative using our highly sensitive AlphaLISA^® assay kits
Cellular epigenetic marks: Our epigenetic assay kits, alternatives to an ELISA assay kit, enable rapid and direct detection of endogenous modification of epigenetic targets
Cellular phosphorylation: Count on our technology for rapid, sensitive, and quantitative detection of protein phosphorylation from cells
Multiplexing assays: Use our highly sensitive and highly reproducible technology for all-in-one-well multiplexing

Products & Services (651)
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cAMP (1)
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Agriculture/Plant (3)
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Alpha SureFire CETSA (10)
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Alpha SureFire Ultra Multiplex (1)
AlphaLISA (337)
AlphaLISA SureFire Ultra (195)
AlphaLISA SureFire Ultra Multiplex (57)
AlphaPlex (18)
AlphaScreen (15)
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DELFIA TRF (1)

Detection Method

Alpha (631)

Molecular Modification

Acetylation (3)
Methylation (6)
Phosphorylation (23)
Total (1)
Unmodified (1)

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1-25 of 651 Products & Services

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Total ZAP-70 AlphaLISA SureFire Ultra Detection Kit

ALSU-TZAP-A-HV , ALSU-TZAP-A500 , ALSU-TZAP-A10K , ALSU-TZAP-A50K

The AlphaLISA^® SureFire^® Ultra™ total ZAP-70 assay is a sandwich immunoassay for quantitative detection of total ZAP-70 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay in detection of phospho-ZAP-70.

Price Range : 553.00 USD - 36700.00 USD
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Total c-Myc AlphaLISA SureFire Ultra Detection Kit

ALSU-TCMYC-A500 , ALSU-TCMYC-A10K , ALSU-TCMYC-A50K , ALSU-TCMYC-A-HV

The AlphaLISA® SureFire® Ultra™ total c-myc assay is a sandwich immunoassay for quantitative detection of c-myc (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization phosphorylation studies.

Price Range : 553.00 USD - 36700.00 USD
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Akt1 Alpha SureFire CETSA Target Engagement Assay Kit

CETSA-AKT1-A500 , CETSA-AKT1-A5K1 , CETSA-AKT1-A5K2 , CETSA-AKT1-A5K3 , CETSA-AKT1-A5K4 , CETSA-AKT1-A5K5

Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire^® CETSA^® target engagement assays. For use in combination with Pelago Bioscience's CETSA^® (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to AKT1, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA^® subscription from Pelago Biosciences is required for use.

Price Range : 2559.00 USD - 7800.00 USD
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IL-4 (porcine) AlphaLISA Detection Kit

AL578HV , AL578C , AL578F

The AlphaLISA^® immunoassay kit for porcine interleukin 4 (IL-4) enables the quantitative determination of porcine IL-4 in cell culture media and serum using a homogeneous AlphaLISA assay (no wash steps).

Price Range : 939.00 USD - 18600.00 USD
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IFN-β (human) AlphaLISA Detection Kit

AL3133C , AL3133F

The AlphaLISA® Human Interferon β (IFN-β) Detection Kit is designed for detection and quantitation of human IFN-β in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit corresponds to the IFN-β 1a subtype.

This product replaces AL265.

Price Range : 1905.00 USD - 12600.00 USD
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BCL-2 Alpha CETSA Target Engagement Assay Kit

CETSA-BCL2-A500 , CETSA-BCL2-A5K1 , CETSA-BCL2-A5K2 , CETSA-BCL2-A5K3 , CETSA-BCL2-A5K4 , CETSA-BCL2-A5K5

Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha CETSA^® target engagement assays. For use in combination with Pelago Bioscience's CETSA^® (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to BCL-2, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA^® subscription from Pelago Biosciences is required for use.

Price Range : 3962.00 USD - 34300.00 USD
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BTK (human) AlphaLISA Detection Kit

AL3110HV , AL3110C , AL3110F

The AlphaLISA^® immunoassay kit for human Bruton's tyrosine kinase (BTK) enables the quantitative determination of human BTK in buffer and cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Price Range : 940.00 USD - 13300.00 USD
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Phospho-Cofilin (Ser3) AlphaLISA SureFire Ultra Detection Kit

ALSU-PCOF-A-HV , ALSU-PCOF-A500 , ALSU-PCOF-A10K , ALSU-PCOF-A50K

The AlphaLISA^® SureFire^® Ultra^™ p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.

Price Range : 553.00 USD - 35300.00 USD
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AlphaScreen General IgG (Protein A) Detection Kit

6760617HV , 6760617C , 6760617M , 6760617R

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.

Price Range : 113.00 USD - 17300.00 USD
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Alpha SureFire^® Ultra™ Multiplex phospho/total NFκB Assay Kit

MPSU-PTNFKB-K-HV , MPSU-PTNFKB-K500 , MPSU-PTNFKB-K10K , MPSU-PTNFKB-K50K

The Alpha SureFire^® Ultra™ Multiplex phospho-NFκB p65 (Ser536) + Total NFκB p65 assay kit is used to measure both the phosphorylation (on Ser536) and total levels of endogenous NFκB in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure NFκB phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

Price Range : 995.00 USD - 63800.00 USD
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AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

AL720HV , AL720C , AL720F

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Price Range : 871.00 USD - 15600.00 USD
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AlphaScreen cAMP Detection Kit

6760635D , 6760635M , 6760635R

AlphaScreen no-wash assay kit for quantitative detection of cAMP.

Price Range : 505.00 USD - 17400.00 USD
New

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AlphaLISA SureFire Ultra Human and Mouse Phospho-ATG16L1 (Ser278) Detection Kit

ALSU-PATG-A500 , ALSU-PATG-A10K , ALSU-PATG-A50K , ALSU-PATG-A-HV

The AlphaLISA^® SureFire^® Ultra^™ Human and Mouse Phospho-ATG16L1 (Ser278) assay is a sandwich immunoassay for quantitative detection of phospho-ATG16L1 (Ser278) in cellular lysates using Alpha Technology.

Price Range : 640.00 USD - 32400.00 USD
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PTEN (human) AlphaLISA Detection Kit

AL380HV , AL380C , AL380F

The AlphaLISA^® immunoassay kit for human PTEN enables the detection and quantitation of human PTEN in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps). Other species of PTEN were not tested, but detection is expected with mouse and rat PTEN due to >99% sequence homology.

Price Range : 575.00 USD - 12600.00 USD
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AlphaScreen FITC (Fluorescein) Detection Kit

6760605C , 6760605M , 6760605R

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads.

Price Range : 241.00 USD - 17300.00 USD
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PCSK9/LDLR (human) AlphaLISA Binding Kit

AL3132C , AL3132F

This kit is designed to assess binding and inhibition of human PCSK9 and LDLR, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics using competition assays.

This is a replacement for AL3119.

Price Range : 3008.00 USD - 21500.00 USD
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Phospho-PI3 Kinase p85 (Tyr458) AlphaLISA SureFire Ultra Detection Kit

ALSU-PP13K-A500 , ALSU-PP13K-A10K , ALSU-PP13K-A50K , ALSU-PP13K-A-HV

The AlphaLISA^® SureFire^® Ultra^™ p-PI3 Kinase p85 (Tyr458) (Mouse) assay is a sandwich immunoassay for quantitative detection of phospho-PI3 Kinase p85 (phosphorylated on Tyr158) in cellular lysates using Alpha Technology.

Price Range : 526.00 USD - 34900.00 USD
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ApoA1 (human) AlphaLISA Detection Kit

AL389HV , AL389C , AL389F

The AlphaLISA^® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.

Price Range : 774.00 USD - 23300.00 USD
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AlphaScreen SureFire IkBalpha (p-Ser32/Ser36) Assay Kit

TGRIKS500 , TGRIKS10K , TGRIKS50K

The AlphaScreen^® SureFire^® p-IκBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IκBα (phosphorylated on Ser32/36) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Price Range : 1699.00 USD - 18300.00 USD
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AlphaPlex-645 (Sm) Human ICAM-1 Detection Kit

AP282SM-HV , AP282SM-C , AP282SM-F

The AlphaPlex™-645 (Sm) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).

Price Range : 807.00 USD - 28500.00 USD
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AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit

ALSU-PRIP3-A500 , ALSU-PRIP3-A10K , ALSU-PRIP3-A50K , ALSU-PRIP3-A-HV

The AlphaLISA^® SureFire^® Ultra^™ Human Phospho-RIP3 (Ser227) assay is a sandwich immunoassay for quantitative detection of phospho-RIP3 (Ser227) in cellular lysates using Alpha Technology.

Price Range : 640.00 USD - 32400.00 USD
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Total EGFR AlphaLISA SureFire Ultra Detection Kit

ALSU-TEGFR-A-HV , ALSU-TEGFR-A500 , ALSU-TEGFR-A10K , ALSU-TEGFR-A50K

The AlphaLISA^® SureFire^® Ultra™ total EGFR assay is a sandwich immunoassay for quantitative detection of EGFR (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-EGFR assay.

Price Range : 553.00 USD - 36700.00 USD
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FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

AL347HV , AL347C , AL347F

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA^® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.

Price Range : 731.00 USD - 13300.00 USD
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Total Cofilin AlphaLISA SureFire Ultra Detection Kit

ALSU-TCOF-A-HV , ALSU-TCOF-A500 , ALSU-TCOF-A10K , ALSU-TCOF-A50K

The AlphaLISA^® SureFire^® Ultra^™ Total Cofilin assay is a sandwich immunoassay for quantitative detection of cofilin (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

Price Range : 553.00 USD - 35300.00 USD
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Factor VIII (human) AlphaLISA Detection Kit

AL342HV , AL342C , AL342F

The AlphaLISA^® Human VIII Detection Kit is designed for detection and quantitation of human VIII in cell culture media or serum using a homogeneous (no-wash steps, no separation steps) assay.

Price Range : 808.00 USD - 12600.00 USD