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Alpha Kits

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Alpha kits are ready to use for quick optimization and execution of assays for a variety of targets. Alpha technology, an alternative to a traditional ELISA assay kit, enables the detection and screening of molecules-of-interest in a no-wash, highly sensitive, quantitative assay.

  • Cytokine and biomarker detection: Avoid a troublesome, traditional ELISA reagent kit with a far superior alternative using our highly sensitive AlphaLISA® assay kits
  • Cellular epigenetic marks: Our epigenetic assay kits, alternatives to an ELISA assay kit, enable rapid and direct detection of endogenous modification of epigenetic targets
  • Cellular phosphorylation: Count on our technology for rapid, sensitive, and quantitative detection of protein phosphorylation from cells
  • Multiplexing assays: Use our highly sensitive and highly reproducible technology for all-in-one-well multiplexing

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1-25 of 612 Products & Services

  • Alpha Terbium SureFire Ultra Multiplex MPSU
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    Alpha SureFire® Ultra™ Multiplex phospho/total SYK Assay Kit

    MPSU-PTSYK-K-HV , MPSU-PTSYK-K500 , MPSU-PTSYK-K10K , MPSU-PTSYK-K50K
    The Alpha SureFire® Ultra™ Multiplex phospho-SYK (Tyr525/526) + Total SYK assay kit is used to measure both the phosphorylation (on Tyr525/526) and total levels of endogenous SYK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SYK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaLISA Binding
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    PD-1/PD-L1 (human) AlphaLISA Binding Kit

    AL356HV , AL356C , AL356F
    The AlphaLISA® human PD-1/PD-L1 binding kit is designed for the detection of binding activity between human PD-1 and PD-L1, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 828.00 USD - 11600.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    SCF (human) AlphaLISA Detection Kit

    AL3019HV , AL3019C , AL3019F
    The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • AlphaLISA SureFire Ultra Phospho-protein
    New
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    Phospho-Ret (pan Tyr) AlphaLISA SureFire Ultra Detection Kit

    ALSU-PRET-A500 , ALSU-PRET-A10K , ALSU-PRET-A50K , ALSU-PRET-A-HV

    The AlphaLISA® SureFire® Ultra™ p-Ret (pan Tyr) assay is a sandwich immunoassay for quantitative detection of phospho-Ret (pan Tyr phosphorylated) in cellular lysates using Alpha Technology.

    Price Range : 526.00 USD - 34900.00 USD
  • AlphaLISA Biotin Free DIG
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    IFN-γ (human) AlphaLISA Biotin-Free Detection Kit

    AL327HV , AL327C , AL327F
    The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
    Price Range : 828.00 USD - 12000.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    IL-25 (human) AlphaLISA Detection Kit

    AL3048HV , AL3048C , AL3048F
    The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 715.00 USD - 17200.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    Mesothelin (human) AlphaLISA Detection Kit

    AL3063HV , AL3063C , AL3063F
    The AlphaLISA® immunoassay kit for human mesothelin enables the quantitative determination of human mesothelin (CAK1, ERC) in serum, buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    IFNα (porcine) AlphaLISA Detection Kit

    AL576HV , AL576C , AL576F
    The AlphaLISA® immunoassay kit for porcine interferon α (IFNα) enables the quantitative determination of porcine IFNα in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 1268.00 USD - 12300.00 USD
  • AlphaScreen Anti-Species
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    AlphaScreen Phosphotyrosine (PY20) Assay Kit

    6760601C , 6760601M , 6760601R
    AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
    Price Range : 214.00 USD - 16400.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    Perlecan (human) AlphaLISA Detection Kit

    AL377HV , AL377C , AL377F
    The AlphaLISA® immunoassay kit for human perlecan enables the detection and quantitation of human perlecan (endorepellin) in human serum and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay showed no cross reacitivity to vimentin, fibronectin, nidogen-1, laminin, or collagen I.
    Price Range : 531.00 USD - 11600.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    HVEM (human) AlphaLISA Detection Kit

    AL3054HV , AL3054C , AL3054F
    The AlphaLISA® immunoassay kit for human herpesvirus entry mediator (HVEM) enables the quantitative determination of human HVEM (also known as TNFRSF14, CD270) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • Photo of Alpha CETSA 500 point kit
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    CDK2 Alpha SureFire CETSA Target Engagement Assay Kit

    CETSA-CDK2-A500 , CETSA-CDK2-A5K1 , CETSA-CDK2-A5K2 , CETSA-CDK2-A5K3 , CETSA-CDK2-A5K4 , CETSA-CDK2-A5K5

    Measure binding of small molecule drug candidates to target protein in a cellular environment with Alpha SureFire® CETSA® target engagement assays. For use in combination with Pelago Bioscience's CETSA® (Cellular Thermal Shift Assay) technology, this kit provides a convenient no-wash assay for detection and quantitation of small molecule binding to CDK2, and can be used in hit identification, hit confirmation, and lead optimization studies for increased confidence and better prediction early in the drug discovery process. Please note that a valid CETSA® subscription from Pelago Biosciences is required for use.

    Price Range : 2432.00 USD - 7400.00 USD
  • AlphaScreen Anti-Species
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    CTLA4-CD86 AlphaLISA Binding Kit

    AL3047C , AL3047F
    The AlphaLISA® CTLA-4/CD86 binding kit is designed for the detection of binding activity between human CTLA-4 and CD86, using a fast and simple homogeneous AlphaLISA assay (no wash steps). This assay can be used to screen for small molecules that inhibit binding, as a competitive ligand binding (CLB) assay to screen therapeutic blocking antibodies, and for potency assays.
    Price Range : 2144.00 USD - 13100.00 USD
  • AlphaLISA SureFire Ultra Phospho-protein
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    AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

    ALSU-PST5-B-HV , ALSU-PST5-B500 , ALSU-PST5-B10K , ALSU-PST5-B50K
    The AlphaLISA® SureFire® Ultra™ p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
    Price Range : 526.00 USD - 33600.00 USD
  • AlphaLISA SureFire Ultra total-protein
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    Total eIF2α AlphaLISA SureFire Ultra Detection Kit

    ALSU-TEIF2-A-HV , ALSU-TEIF2-A500 , ALSU-TEIF2-A10K , ALSU-TEIF2-A50K
    The AlphaLISA® SureFire® Ultra™ total eIF2α assay is a sandwich immunoassay for quantitative detection of eIF2α (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-eIF2α assay.
    Price Range : 526.00 USD - 34900.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    Kappa light chain (human) AlphaLISA Detection Kit

    AL3023HV , AL3023C , AL3023F
    The AlphaLISA® immunoassay kit for human kappa light chain enables the quantitative determination of human kappa light chain in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 515.00 USD - 11300.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    BTLA (human) AlphaLISA Detection Kit

    AL3062HV , AL3062C , AL3062F
    The AlphaLISA® immunoassay kit for human BTLA enables the quantitative determination of human B- and T-lymphocyte attenuator (BTLA, CD272) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 893.00 USD - 17200.00 USD
  • AlphaLISA SureFire Ultra total-protein
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    AlphaLISA SureFire® Ultra™ total PD-1 Assay Kit

    ALSU-TPD1-A-HV , ALSU-TPD1-A500 , ALSU-TPD1-A10K , ALSU-TPD1-A50K
    The AlphaLISA® SureFire® Ultra™ total PD-1 assay kit can be used to measure levels of PD-1 (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.
    Price Range : 526.00 USD - 34900.00 USD
  • AlphaScreen Anti-Species
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    AlphaScreen GST Detection Kit

    6760603C , 6760603M , 6760603R
    AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
    Price Range : 214.00 USD - 16400.00 USD
  • SureFire principle
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    AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

    TGRAF2S500 , TGRAF2S10K , TGRAF2S50K
    The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
    Price Range : 1615.00 USD - 17400.00 USD
  • AlphaPlex Samarium immunoassay kits
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    AlphaPlex-645 (Sm) Human Insulin Detection Kit

    AP204SM-HV , AP204SM-C , AP204SM-F
    The AlphaPlex™ 645 (Samarium) Human Insulin Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
    Price Range : 549.00 USD - 12000.00 USD
  • Alpha Terbium SureFire Ultra Multiplex MPSU
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    Alpha SureFire® Ultra™ Multiplex phospho/total BTK Assay Kit

    MPSU-PTBTK-K-HV , MPSU-PTBTK-K500 , MPSU-PTBTK-K10K , MPSU-PTBTK-K50K
    The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
    Price Range : 946.00 USD - 60600.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    IL-1α (bovine) AlphaLISA Detection Kit

    AL537HV , AL537C , AL537F
    The AlphaLISA immunoassay kit for detection and quantitation of bovine IL1α in serum, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
    Price Range : 768.00 USD - 12300.00 USD
  • AlphaLISA Biotin Free DIG
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    IL-15 (human) AlphaLISA Biotin-Free Detection Kit

    AL360HV , AL360C , AL360F
    The AlphaLISA® Human IL-15 Biotin-Free Detection Kit is designed for the quantitative detection of human Interleukin 15 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
    Price Range : 531.00 USD - 11600.00 USD
  • AlphaLISA Sandwich Anti-AnalyteConjugatedAcceptorBead
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    TIM-3 (human) AlphaLISA Detection Kit

    AL3052HV , AL3052C , AL3052F
    The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
    Price Range : 715.00 USD - 17200.00 USD
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