Alpha Acceptor beads are used in conjunction with Alpha Donor beads to create homogeneous assays (no wash steps, no separation steps) for your application. AlphaLISA Technology: the flexible choice for biochemical and cellular assay creation.
Measure a broad range of protein-protein and other binding interactions, from low-affinity to high-affinity (pM to mM)
Detect and quantitate any analyte, from small molecules to very large protein and antibody complexes - spanning up to 200 nm or more
Screen and quantitate various enzymatic reactions, from kinase to epigenetic targets
Unconjugated AlphaLISA Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaLISA Acceptor beads for use in Alpha no-wash assays.
AlphaPlex™-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
AlphaLISA® Acceptor beads conjugated to anti-mouse IgM antibody. The antibody is a monoclonal rat antibody that reacts with the μ heavy chain of mouse IgM. These beads can be used to capture mouse IgM antibodies for AlphaLISA no-wash assays.
AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
The Streptavidin coated AlphaPlex™ 645 (Samarium) Acceptor beads are used to capture a wide variety of biotinylated reagents, ranging from small nucleotides to very large proteins. To avoid steric hindrance, it is recommended to use biotin derivatives including an aliphatic spacer composed of at least six carbons.
AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.