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VICTOR Nivo Multimode Microplate Reader

Now with Alpha Technology, the VICTOR® Nivo system packs all popular detection modes into the industry’s smallest multimode reader footprint. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all standard technologies (top only reading for Alpha). Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser environments. Combine with our assays and reagents to get you started right away.

For research use only. Not for use in diagnostic procedures.

Part Number HH35000500
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For research use only. Not for use in diagnostic procedures.


The VICTOR Nivo system is a high-performance filter-based multimode microplate reader equipped with all major detection technologies:

  • Absorbance
  • Luminescence
  • Fluorescence intensity
  • Time-resolved fluorescence
  • Fluorescence polarization
  • Alpha

It’s a compact, light-weight multimode plate reader designed for life science research laboratories performing routine low-throughput assays, or assay development work, with diverse application requirements.

The VICTOR NIVO system’s software has a modern, workflow-oriented user interface which is easy to learn and use and includes pre-written application protocols to get users productive quickly. In addition, MyAssays Desktop Standard software is provided for data analysis. With our Enhanced Security software option, it contains technological tools to help guide you through the complexity of compliance for 21 CFR Part 11.

The browser-based control software is independent of operating system, so it can be controlled via a variety of touchscreen devices, for example Windows or Mac OS PCs or tablets.

The VICTOR Nivo microplate reader can be operated via WiFi or a network connection, enabling experiments to be set-up, run and monitored remotely, such as from outside a biosafety cabinet or from a neighbouring lab.

Detection Technologies

At the heart of the VICTOR Nivo multimode plate reader is a dynamic wheel system with space for up to 32 filters, enabling the detection of a multitude of different dyes. Filters are exchanged between the inner and outer wheels, so any individual filter can serve either the excitation or emission light path. As a result, there’s no need to install new filters when switching between assays, and filters are locked in place, so they can’t get lost in the lab – ideal for multi-user environments. When fully-loaded, the filter system provides the flexibility to detect many dyes with better sensitivity and greater cost-effectiveness than a monochromator.

For absorbance measurements using the VICTOR Nivo multimode plate reader, there is a choice of either a filter- or a spectrometer-based system. With the spectrometer-based plate reader, full spectrum absorbance measurements are ultra-fast – 230 to 1000 nm at selectable resolutions (2.0 nm, 5.0 nm, 10 nm) in less than 1 second per well.

Up to eight discrete wavelengths can be detected at once in a single measurement with no wavelength switching, and the spectrometer-based VICTOR Nivo system also allows the detection of a wide range of dyes or measurement of samples with unknown absorbance spectra.

Features & Benefits

  • Compact, lightweight multimode plate reader which frees-up bench space and is easy to move into a biosafety cabinet or another lab
  • Internal dynamic filter wheel system, with space for up to 32 filters, provides ready access to filters for a large number of dyes
  • For absorbance, choice of filter-based detection for best sensitivity or spectrometer for wavelength flexibility
  • Time resolved fluorescence certified for use with HTRF® technology
  • Top and bottom reading of all standard technologies (absorbance, luminescence, fluorescence intensity, time-resolved fluorescence and fluorescence polarization), top reading only for Alpha; with high performance for plate formats from 1 to 1536-wells
  • Enhanced Security software for regulated environments provides technological controls and features that support 21 CFR Part 11 compliance
  • Optional dispenser for applications such as fast kinetics, flash luminescence assays, or dual addition assays
  • Integrated temperature control (up to 65 °C) and optional gas control unit to keep cells viable during long term kinetic assays
  • Browser-based software enables the plate reader to be controlled from a variety of devices – PC, laptop or tablet
  • Can be controlled via network or WiFi to facilitate remote working and data access


Depth 50.0 cm
Detection Method Absorbance, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence Intensity, Fluorescence Polarization
Height 27.0 cm
Portable Yes
Product Brand Name VICTOR Nivo
Weight 13.0 kg
Width 20.0 cm


Modalities VICTOR Nivo F VICTOR Nivo S VICTOR Nivo Alpha F VICTOR Nivo Alpha S Standard Features Optional Extras
(Filter-based (F) or Spectrometer (S))
  • Top and bottom detection of all technologies (except Alpha) for plate formats up to 1536-wells
  • Temperature control up to 65°C
  • Shaker with three modes (linear, orbital, and double orbital)
  • Browser-based control software
  • Wi-Fi modem for remote control
  • MyAssays Desktop Standard analysis software
  • Dispenser (dual injectors)
  • Gas control (CO2 & O2) Automation Control SDK
  • 15-in. laptop
  • My Assays Desktop Pro analysis software
  • Enhanced Security software supporting 21 CFR Part 11 compliance in regulated environments
  • Test plate for alignment and system check
  • Field upgrades from standard to advanced models (includes time-resolved fluorescence and fluorescence polarization)
Fluorescence Intensity
Time-resolved Fluorescence Optional Optional Optional Optional
Fluorescence Polarization Optional Optional Optional Optional
Resources, Events & More
  • All

Application Note

High-Throughput DNA Quantification for Next Generation Sequencing on the VICTOR Nivo

There are many different methods of quantification available on the market for DNA samples and each can vary in precision, accuracy and throughput. Typically, DNA in next generation sequencing (NGS) workflows is measured using tube-based fluorescent assays such as Qubit® or qPCR. However, since samples have to be measured one at a time, this can create bottlenecks in a high-throughput lab. Using an automated liquid handler and plate reader instead of manually pipetting tube-based measurements can significantly decrease measurement time and increase throughput.

Download our application note to learn how we obtain consistent, reproducible and high-throughput results using the VICTOR® Nivo multimode plate reader in conjunction with the Zephyr G3 NGS workstation to quantify DNA with reagents from Quant-iT® assay kits.



VICTOR Nivo Multimode Plate Reader Brochure

This Brochure describes the VICTOR Nivo Multimode Plate Reader.



Solving the Complexity of Compliance

Reaching compliance according to the laws and regulations of each country can be a tedious, complicated process, and being GxP compliant (FDA 21 CFR Part 11, EU Annex 11, etc.) is necessary in order to deliver therapeutic treatments to market for pharmaceutical and biotechnology companies, including clinical research organizations.

Simplify compliance for your VICTOR® Nivo or EnVision® multimode plate reader with the help of our Enhanced Security software. In addition, our wide range of assays can help you achieve your research goals, and our qualification services will make sure your multimode plate reader and software are operating at the recommended technical specifications.

To guide you through the complexity of the compliance process, we can help with our proven solutions, customer training, and technical support so you can focus on your science and research.


Technical Note

Speed Up Your Immunoassays with AlphaLISA Detection on VICTOR Nivo

Using ELISA technology for immunoassays on a plate reader is laborious and time-consuming because it requires numerous wash steps. In the field of drug discovery, technologies that give robust, sensitive, automatable, and reproducible immunoassays at a lower cost are highly desirable.

Alpha Technology simplifies an assay workflow and accelerates time-to-results because no wash steps are needed. Learn more on the advantage of AlphaLISA® with its quicker time-to-results, simplified assay workflow, and improved assay robustness and reproducibility on the VICTOR® Nivo multimode plate reader, now with Alpha detection.

PDF 975 KB

White Paper

Guidance for Ensuring Your VICTOR Nivo Multimode Plate Reader Complies with 21 CFR Part 11

Ensuring that laboratory processes are compliant with the regulations of the U.S. Food and Drug Administration (FDA), such as 21 CFR Part 11, and other references, e.g. Annex 11 published by the European Commission, can be time-consuming and laborious, requiring meticulous documentation of procedures and record-keeping.

To help ensure that procedures which involve the VICTOR® Nivo multimode plate reader are compliant, an add-on to the instrument's software is available. Named the Enhanced Security option, this software provides a variety of tools to help you reach compliance for 21 CFR Part 11. With specific reference to the relevant paragraphs of 21 CFR Part 11, this document describes how the tools within the VICTOR Nivo Enhanced Security software facilitate compliance with this regulation.