LANCE® Eu-W1024-ITC Chelate is optimized for the labeling of proteins and peptides at least one primary aliphatic amino group. The labeled compound can be used in homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays (LANCE® assays).
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
Automation Compatible | Yes |
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Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
Experimental Type | In vitro |
Fluorophore | Eu-W1024-ITC |
Product Brand Name | LANCE |
Shipping Condition | Blue Ice |
Unit Size | 1 mg |
Fluorescent isothiocyanato-activated (ITC-activated) Eu-W1024 chelate is optimized for labeling proteins and peptides containing at least one aliphatic amino group.