PerkinElmer
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ProxiPlate-384 Plus, White 384-shallow well Microplate, Case 50

384-well microplate for use in time-resolved fluorescence, luminescence, Alpha, and radiometric assays. This white, polystyrene ProxiPlate™ has shallow wells allowing for lower volumes to be used in assays.

Part Number
Unit Size
List Price
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Quantity
6008280
Case of 50
448.00 USD
 
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6008289
Case of 200
1494.00 USD
 
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Overview

The ProxiPlate is PerkinElmer's shallow-well microplate, offering closer proximity to the analyzer's detectors. Designed to miniaturize your assay and utilize cost reduction advantages.

Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better microplates for better performance that guarantees better results for all PerkinElmer applications.

Packaging Notes:

  • Cases of 50 are packaged as 2 sleeves of 25 plates each. Each sleeve as 5 wraps with 5 plates.
  • TC-treated cases of 50 are packaged as 50 individually bagged and sealed lidded plates.
  • TC-treated cases of 160 are packaged as 8 sleeves of 20 lidded plates each. Each sleeve as 5 wraps of 4 lidded plates.
  • Cases of 200 are packaged as 8 sleeves of 25 plates each. Each sleeve as 5 wraps with 5 plates.
  • TC-treated cases of 200 are packaged as 8 sleeves of 25 plates each. Each sleeve as 5 wraps with 5 plates. Lids are not included but can be ordered separately as Cat. No. 6005619 for 96-well plates or Cat. No. 6007619 for 384-well plates.

Well Plate Dimensions

Well format: 384-well

Description Specification
Number of Rows 16
Number of Columns 24
Well volume 28 µL
Recommended working volume 10 µL- 20 µL
Height (mm) 14.35
Length (mm) 127.76
Width (mm) 85.48
Well diameter (mm) 3.3
Well depth (mm) 5.3
A1 to top offset (mm) 8.99
A1 to side offset (mm) 12.13
Well-to-well spacing (mm) 4.5

Specifications

Automation Compatible Yes
Coating Treatment Untreated
Color White
Detection Method Luminescence, Alpha, Time-Resolved Fluorescence (TRF & TR-FRET), Radiometric
Material Polystyrene
Product Brand Name ProxiPlate
Shipping Condition Ambient
Sterile No
Unit Size Case of 50
Wells Number 384 well plate
Well Shape Shallow-well
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Application Note

Determination of Cytokines Present in a CAR-T Co-Culture Environment by AlphaLISA and HTRF Technologies

While fundamental knowledge about tumor immunology has exploded recently, a new therapeutic approach to cancer is taking off: immunotherapy. Instead of directly attacking tumor cells, the idea is to help the immune system recognize and destroy them.

The use of CAR-T cells (Chimeric Antigen Receptor-T Cells), a new avenue of immunotherapy, consists in genetically modifying the patient's immune cells to arm them against a tumor. Concretely, T lymphocytes are taken from the patient's blood and modified in vitro. This leads to their expression of specific surface receptors, which recognize a tumor antigen. Once modified, these CAR-T cells are multiplied and re-injected into the patient's body in large quantities. There they go on to destroy cancer cells after binding to the tumor antigen, releasing a mixture of cytokines and pro-inflammatory chemokines.

This application note focuses on detecting cytokine and chemokine secretion using two orthogonal no-wash immunoassays, AlphaLISA® and HTRF®, in an in vitro co-culture model with CAR-T cells and CD19 positive Raji cells targeting tumors.

PDF 1 MB

Data Sheet