The MuviCyte live-cell imaging system is designed to operate inside your cell-culture incubator, enabling you to maintain your cells under optimal conditions and perform a wide range of assays in a variety of culture vessels.
Life science research labs study cellular behaviors and pathways to gain a deeper understanding of functions, disease mechanisms and responses to treatments. Live-cell imaging is a vital tool for getting maximum information from precious cell samples.
The MuviCyte live-cell imaging kit consists of a MuviCyte live-cell imaging instrument plus 4x, 10x and 20x objective lenses, PC and monitor.
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For research use only. Not for use in diagnostic procedures.
The MuviCyte system is designed to operate inside your incubator, so you can maintain your cells under optimal conditions, keeping them healthy for weeks at a time. Controlled by an external PC, you can observe your cells remotely, which helps to keep the culture chamber at optimum levels of temperature, CO2, and humidity. The automated operation allows you to focus on your science while the instrument runs unattended.
With three-color fluorescence imaging, z-stacking, and stitching capabilities, you can perform a wide range of assays in a variety of culture vessels, e.g. chamber slides, petri dishes, flasks, microplates. Automated imaging, over hours to days, means your assays can be run at much higher throughput than with a traditional microscope.
The system has built-in image analysis software for assays such as cell health and viability, transfection efficiency, reporter gene, apoptosis and proliferation, and optional software available for scratch wound and spheroid assays.
The flexible movie-making software provides a great way to gain more realistic and meaningful insights into cell behavior, functions, and responses.
Features and benefits
Typical applications and assays
|Detection Method||Fluorescence, Brightfield|
|Imaging Modality||Live-cell imaging|
|Product Brand Name||MuviCyte|
Live-cell imaging, the study of living cells using microscopy, has become a requisite technology in many fields of biomedical research, such as cell biology, developmental biology and cancer research. Also, in drug discovery, researchers adopt live-cell imaging as they look for a more detailed understanding of cellular behavior.
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Live-cell imaging has evolved from allowing observation of large-scale changes to capturing subtle changes in dynamic cellular processes. Today, live-cell imaging coupled with high-content analysis enables researchers to extract quantitative data in real-time, facilitating new insights in basic life science research and drug discovery. Modern live-cell imaging systems can capture rapid cellular events, track cell movement, monitor protein signaling, screen cell health, and much more.
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To study cell migration dynamics or to identify drugs affecting cell migration, the scratch wound assay is one of the most prominent and popular in vitro methods.
As experimental procedures used in such assays are highly variable and lack standardization, results from different experiments or sources are often difficult to compare.
Download our tech note to learn how you can perform a kinetic, label-free, live-cell scratch wound assay, which is robust and reliable with the MuviCyte™ live-cell imaging system.
Cytotoxicity assays are often performed as endpoint assays, but a kinetic or time-resolved assay enables more precise assessment of cytotoxicity, especially if the kinetics of the compounds are unknown or varied, or if cells display different proliferation rates.
Assessment of cytotoxicity can also be affected by the type of cell model, e.g. 2D or 3D, or the use of fluorescent dyes which, over a long time-course, can have negative effects on metabolism and proliferation and hence on responses to the exogenous factors.
In this technical note, we describe label-free analysis of cell growth in monolayer and spheroid growth in 3D using brightfield images in order to determine cytotoxic effects, and compare the responses of three different cell lines to test compounds in both 2D and 3D.
Download our technical note to learn how you can: