PerkinElmer
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BackSeal-96/384, White Adhesive Bottom Seal for 96-well and 384-well Microplate

White adhesive seal for the bottom of microplates. BackSeal is used in conjunction with clear-bottom plates to turn the bottom of a microplate opaque.

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Overview

White adhesive seal for the bottom of microplates. BackSeal is used in conjunction with clear-bottom plates, such as the ViewPlate™ or CellCarrier™ plate, to turn the bottom of a microplate opaque.

Specifications

Color White
Product Brand Name BackSeal
Shipping Condition Ambient
Unit Size Pack of 55
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Application Note

Using AlphaLISA Biomarker Kits to Assess Effects of PBMC-Conditioned Media on 3D and 2D Cell Culture Models of Breast Cancer

Various cytokines are secreted during an active immune response that can have modulatory effects on target cell populations, including interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNFa) and several interleukins.

In this application note, you will learn how we investigated:

  • The effects of secreted factors on a triple-negative breast cancer cell line by treatment with conditioned media from activated PBMCs
  • Whether the modulatory effects of cytokines secreted by infiltrating immune is different when studied in 3D cell cultures
  • How to detect and quantify multiple immune-modulated proteins from the same wells in complex cell models grown in 3D spheroid microplates and traditional 2D cell culture using AlphaLISA® technology and EnVision® multimode plate reader in a workflow with ATPlite 1step and Opera® Phenix high-content screening system

PDF 2 MB