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AlphaScreen General IgG (Protein A) Detection Kit, 500 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
100 assay points
113.00 USD
500 assay points
225.00 USD
10,000 assay points
3606.00 USD
50,000 assay points
17300.00 USD
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This bead kit can be used for many applications, including:

  • Antibody detection
  • Antibody binding
  • Binding assays
  • Enzymatic assays

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction.  This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.


Antibody Conjugates Streptavidin/Protein A
Automation Compatible Yes
Product Brand Name AlphaScreen
Shipping Condition Blue Ice
Unit Size 500 assay points
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.

Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.


Application Note

AlphaScreen™ MAP Kinase Assay

AlphaScreen™ MAP Kinase Assay was designed to measure ERK1/2 MAP kinase activity present in purified enzyme preparations or in cell extracts. The assay was developed using a very active ERK1 preparation. However, it can easily be applied to ERK1 extracted from other sources and to other MAP kinases.

PDF 605 KB


Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.



Development of a Homogeneous p38 Kinase Assay using AlphaScreen™ Technology

AlphaScreen™ is a homogeneous, luminescent proximity assay useful for studying a wide variety of biomolecular interactions. Here, we report the development of an AlphaScreen p38 kinase assay by monitoring the phosphorylation of activating transcription factor 2 (ATF-2).

PDF 275 KB