PerkinElmer

Reporter Gene

OF

For cost-effective automated screening and batch plate processing our luciferin-based reporter gene systems produce a long-lived glow type signal with a half-life of several hours.

  • Reporter gene assays with easily measurable phenotypes
  • Characterize the strength of promoters and enhancers
  • Define the role of transcription factors
  • Assess transfection efficiency
  • Convenient one-step processing: No washes required -- simply mix, incubate and read
  • Strong signal for remarkably accurate and thorough firefly luciferase reading
  • Enhanced stability: All our luciferase-based reagents are designed for extended storage at 2-8°C
  • No DTT: Unlike most luciferase detection reagents, ours do not contain DTT, eliminating toxic hazards and the need for hood work
  • Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
  • Excellent Z' Values: All systems offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

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Application
  • Molecular Biology (10)
Product Brand Name
  • britelite (1)
  • EasyTag (1)
  • NEN Radiochemicals (42)
  • neolite (1)
  • sensilite (2)
  • steadylite plus (1)
  • twinlite (2)
Detection Method
  • Luminescence (6)
  • Radiometric (54)
Radioisotope
  • C-14 (18)
  • H-3 (27)
  • S-35 (10)
Radionuclide
  • 14C (18)
  • 35S (10)
  • 3H (27)
Label Position
  • Specifically Labeled (41)
  • Uniformly Labeled (12)
Buffer
  • Aqueous (4)
  • Ethanol : water (1)
  • Ethanol:water (1)
  • HCl (1)
  • Sulfuric acid:ethanol (2)
  • Tricine (2)
  • Tricine with stabilizer and dye (1)
Assay points(200-50,000)
  • 100 (3)
  • 1000 (3)
  • 10000 (1)
Automation Compatible
  • Yes (6)
Concentration
  • 0.02 mCi/mL (2)
  • 0.05 mCi/mL (5)
  • 0.1 mCi/mL (11)
  • 0.55 mCi/mL (2)
  • 1 mCi/mL (24)
  • 10.2 mCi/mL (1)
  • 10.25 mCi/mL (1)
  • 11 mCi/mL (5)
  • 2 mCi/mL (1)
  • 31.5 mCi/mL (1)
  • 43.3 mCi/mL (1)
  • 5 mCi/mL (1)
Experimental Type
  • In vitro (6)
Format
  • Microplates (6)
Pipetting Arms
Volume
  • 0-50 (19)
  • 201-250 (16)

"Reporter Gene" 1-50 of 61 Products & Services

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Adenosine 5 Triphosphate 250 µCi

Leucine, L-[3,4,5-3H(N)]-, Specific Activity: 100 to 150 Ci/mmol

L-leucine, nominally labeled with tritium at C3, C4, C5. 3H-L-leucine is commonly used in leucine metabolism and protein synthesis studies.
Adenosine 5 Triphosphate 250 µCi

L-[Methyl-3H]-Methionine

L-methionine, labeled with tritium on the methyl group. 3H-L-methionine is commonly used in methionine metabolism and amino acid uptake studies.
Adenosine 5 Triphosphate 250 µCi

Glutamic Acid, L-[3,4-3H]

L-glutamic acid, labeled with tritium at C3, C4. 3H-L-glutamate is commonly used in glutamate receptor binding, amino acid uptake and release, and protein synthesis studies.

EasyTagTM EXPRESS35S Protein Labeling Mix, [35S]-

Mixture containing both 35S-L-methionine and 35S-L-cysteine, for cell labeling. This mixture is an EasyTag formulation, meaning it can be stored at 4 °C.
Adenosine 5 Triphosphate 250 µCi

Proline, L-[2,3,4,5-3H]

L-proline, labeled with tritium at C2, C3, C4, C5. 3H-L-proline is commonly used in proline metabolism, amino acid uptake and release, and protein synthesis studies.
Adenosine 5 Triphosphate 250 µCi

Tyrosine, L-[Ring-3,5-3H]

L-tyrosine, labeled with tritium on ring carbons 3 and 5. 3H-L-tyrosine is commonly used in protein incorporation and tyrosine hydroxylase assays.
Adenosine 5 Triphosphate 250 µCi

Aspartic Acid, D-[2,3-3H]

D-aspartic acid, labeled with tritium at C2, C3. 3H-D-aspartate is commonly used in aspartate transport and efflux studies.
Diclofenac sodium- Phenylacetic acid C14

Arginine, L-[14C(U)]

L-arginine, uniformly labeled with C-14. 14C-L-arginine is commonly used in arginine metabolism studies, and can be incorporated into proteins in the brain posttranslationally.
Adenosine 5 Triphosphate 250 µCi

Aminohippuric Acid p-[Glycyl-2-3H]-, 1mCi (37MBq)

p-aminohippuric acid (PAH), labeled with tritium on the glycyl group. 3H-PAH is commonly used in clearance and renal plasma flow studies.

Methionine, L-[35S]-, Specific Activity:>1000Ci (37.0TBq)/mMole, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol, and was developed for in vitro translation.
Diclofenac sodium- Phenylacetic acid C14

Valine, L-[U-14C]

L-valine, uniformly labeled with C-14. 14C-L-valine is commonly used to label proteins in incorporation studies, as well as in valine metabolism studies.
Diclofenac sodium- Phenylacetic acid C14

Tryptophan, L-[side chain-3-14C]-,>97%

L-tryptophan, labeled with C-14 on C3 of the side chain. 14C-L-tryptophan is commonly used in tryptophan decarboxylase, amino acid transport, and protein synthesis studies.
Adenosine 5 Triphosphate 250 µCi

Aspartic acid, L-[2,3-3H]-

L-aspartic acid, labeled with tritium at C2,C3. 3H-L-aspartate is commonly used in transporter uptake assays, metabolic assays, and fatty acid labeling.
Diclofenac sodium- Phenylacetic acid C14

Lysine, L-[14C(U)]

L-lysine, uniformly labeled with C-14. 14C-L-lysine is commonly used in histone labeling of chromatin, amino acid uptake, and protein synthesis studies.
neolite Reporter Gene Assay kit

neolite Reporter Gene Assay System

Glow luminescence reporter gene assay combining benefits of both bright signal and long-lasting signal for firefly luciferase.
Diclofenac sodium- Phenylacetic acid C14

Methylaminoisobutyric Acid, α-[1-14C]-

α-Methylaminoisobutyric acid (MeAIB), labeled with C-14 at C1. 14C-MeAIB is a non-metabolizable tracer commonly used in amino acid transporter influx and placental transport studies.
Adenosine 5 Triphosphate 250 µCi

Aminobutyric Acid (GABA), γ-[2,3-3H(N)]-, Specific Activity: 70-100 Ci/mmol

γ-aminobutyric acid (GABA), nominally labeled wih tritium at C2, C3. 3H-GABA is commonly used in neurotransmitter release assays.
Adenosine 5 Triphosphate 250 µCi

Glycine, [2-3H]

Glycine, labeled with tritium at C2. 3H-glycine is commonly used in NMDA receptor binding, glycine uptake, and protein synthesis studies.

EasyTagTM L-[35S]-Methionine, Stabilized Aqueous Solution

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, contains β-mercaptoethanol, and has a blue dye to aid with pipetting.
Adenosine 5 Triphosphate 250 µCi

L-[4,5-3H(N)]-Leucine, Specific Activity 40-60Ci (1.48-2.22TBq)/mmol

L-leucine, labeled with tritium at C4, C5. 3H-L-leucine is commonly used in leucine metabolism and protein synthesis studies.
sensilite enhanced luminescence assay system

sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System

sensilite is an Enhanced Flash Firefly luciferase detection assay system designed to detect and quantitate Firefly luciferase from cultured cells with optimum sensitivity. The assay requires the use of a luminometer equipped with a dispenser as the signal has to be read shortly after dispensing.
Adenosine 5 Triphosphate 250 µCi

Proline, L-[2,3-3H]

L-proline, labeled with tritium at C2, C3. 3H-L-proline is commonly used in proline metabolism, amino acid uptake and release, and protein synthesis studies.
Diclofenac sodium- Phenylacetic acid C14

Glutamine, L-[14C(U)]-

L-glutamine, uniformly labeled with C-14. 14C-L-glutamine is commonly used in glutamine transport studies.

L-[35S]-Methionine, Specific Activity:>800Ci (29.6TBq)/mMole, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.
steadylite plus Reporter Gene Assay kit

steadylite plus Reporter Gene Assay System

Reporter gene assay with longest-lasting signal for quantitation of firefly luciferase in mammalian cells.
Diclofenac sodium- Phenylacetic acid C14

Leucine, L-[14C(U)]-, Specific Activity>300mCi (11.1GBq)/mmol

L-leucine, uniformly labeled with C-14. 14C-L-leucine is commonly used in protein labeling, amino acid uptake, and leucine metabolism studies.
Diclofenac sodium- Phenylacetic acid C14

Alanine, L-[14C(U)]

L-alanine, uniformly labeled with C-14. 14C-L-alanine is commonly used in alanine metabolism studies and studies of protein synthesis, particularly in soil organisms.

Adenosyl-L-methionine, S-[methyl-3H] (SAM[3H]), 12-18 Ci/mmol

Tritiated SAM is commonly used in epigenetic methylation assays.

Cysteine L-[35S]-, 10mM DTT

L-cysteine amino acid, labeled with 35S
Diclofenac sodium- Phenylacetic acid C14

Glutamic Acid, L-[14C(U)]

L-glutamic acid, uniformly labeled with C-14. 14C-L-glutamate is commonly used in glutamate decarboxylase and glutamate uptake assays.
Adenosine 5 Triphosphate 250 µCi

Leucine, L-[4,5-3H]

Leucine (abbreviated as Leu or L) is an essential amino acid is used in metabolism studies.

Methionine, Cell Labeling Grade, L-[35S]-,>600Ci (22.2TBq)mmol, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.
6016802_IMG_1515

Passive Lysis Solution (PLS) 1X, 100 mL

The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and Sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.
twinlite dual luciferase assay system

twinlite Firefly and Renilla Luciferase Reporter Gene Assay System

twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.
britelite plus Reporter Gene Assay kit

britelite plus Reporter Gene Assay System

Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells.
Adenosine 5 Triphosphate 250 µCi

Serine, L-[3H(G)]

L-serine, labeled with tritium. 3H-serine is commonly used in serine metabolism, phospholipid labeling, and protein synthesis studies.
Diclofenac sodium- Phenylacetic acid C14

Tyrosine, L-[U-14C]

Tyrosine is an amino acid used in various protein and receptor studies.

EXPRE35S35S Protein Labeling Mix, [35S]-, 50mM Tricine (pH 7.4), 10mM 2-mercaptoethanol

Mixture containing both 35S-L-methionine and 35S-L-cysteine, for cell labeling.

L-[35S]-Methionine, High radioactive concentration, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation is provided at high radioactive concentration, and contains β-mercaptoethanol.
Adenosine 5 Triphosphate 250 µCi

Spermidine Trihydrochloride [Terminal Methylenes-3H(N)]-

Spermidine, labeled on terminal methylenes with tritium. 3H-spermidine is commonly used in spermidine transport assays.

Methionine, L-[35S], stabilized

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, and contains β-mercaptoethanol.
Adenosine 5 Triphosphate 250 µCi

Aminobutyric Acid (GABA) γ-[2,3-3H(N)]-, Specific Activity: 25-40 Ci/mmol

γ-aminobutyric acid (GABA), nominally labeled wih tritium at C2, C3. 3H-GABA is commonly used in neurotransmitter release assays.
Adenosine 5 Triphosphate 250 µCi

Lysine, L-[4,5-3H(N)]

L-lysine, labeled with tritium at C4, C5. 3H-L-lysine is commonly used in amino acid uptake and protein synthesis studies.
Diclofenac sodium- Phenylacetic acid C14

Serine, L-[3-14C]-

L-serine, labeled with C-14. 14C-serine is commonly used in serine metabolism, phospholipid labeling, and protein synthesis studies.
Adenosine 5 Triphosphate 250 µCi

Phenylalanine, L-[2,3,4,5,6-3H]-

L-phenylalanine, tritiated. 3H-L-phenylalanine is commonly used in protein synthesis studies and protein labeling.
Diclofenac sodium- Phenylacetic acid C14

Phenylalanine, L-[14C(U)]

L-phenylalanine, uniformly labeled with C-14. 14C-L-phenylalanine is commonly used in phenylalanine metabolism, amino acid uptake, and protein synthesis studies.
Adenosine 5 Triphosphate 250 µCi
Adenosine 5 Triphosphate 250 µCi

[Glycine-2-3H]-Glutathione

Glutathione, labeled on the glycine group with tritium. 3H-glutathione is commonly used in binding and transport assays.

Isoleucine, L-[U-14C]

L-isoleucine, uniformly labeled with C-14. 14C-L-isoleucine is commonly used in isoleucine metabolism studies.
Adenosine 5 Triphosphate 250 µCi

Adenosyl-L-Methionine, S-[methyl-3H]-, Specific Activity: 55-85 Ci/mmol

Tritiated SAM is commonly used in epigenetic methylation assays.

"Reporter Gene" 1-50 of 61 Products & Services