PerkinElmer

Reporter Gene

OF

For cost-effective automated screening and batch plate processing our luciferin-based reporter gene systems produce a long-lived glow type signal with a half-life of several hours.

  • Reporter gene assays with easily measurable phenotypes
  • Characterize the strength of promoters and enhancers
  • Define the role of transcription factors
  • Assess transfection efficiency
  • Convenient one-step processing: No washes required -- simply mix, incubate and read
  • Strong signal for remarkably accurate and thorough firefly luciferase reading
  • Enhanced stability: All our luciferase-based reagents are designed for extended storage at 2-8°C
  • No DTT: Unlike most luciferase detection reagents, ours do not contain DTT, eliminating toxic hazards and the need for hood work
  • Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
  • Excellent Z' Values: All systems offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

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Narrow your results
Application
  • Molecular Biology (10)
Assay points(200-50,000)
  • 100 (3)
  • 1000 (3)
  • 10000 (1)
Automation Compatible
  • Yes (6)
Buffer
  • Aqueous (4)
  • Ethanol : water (1)
  • Ethanol:water (1)
  • HCl (1)
  • Sulfuric acid:ethanol (2)
  • Tricine (2)
  • Tricine with stabilizer and dye (1)
Concentration
  • 0.02 mCi/mL (2)
  • 0.05 mCi/mL (5)
  • 0.1 mCi/mL (12)
  • 0.55 mCi/mL (2)
  • 1 mCi/mL (26)
  • 10.2 mCi/mL (1)
  • 10.25 mCi/mL (1)
  • 11 mCi/mL (5)
  • 2 mCi/mL (1)
  • 31.5 mCi/mL (1)
  • 43.3 mCi/mL (1)
  • 5 mCi/mL (1)
Detection Method
  • Luminescence (6)
  • Radiometric (57)
Experimental Type
  • In vitro (6)
Format
  • Microplates (6)
Label Position
  • Specifically Labeled (44)
  • Uniformly Labeled (12)
Product Brand Name
  • britelite (1)
  • EasyTag (1)
  • NEN Radiochemicals (45)
  • neolite (1)
  • sensilite (2)
  • steadylite plus (1)
  • twinlite (2)
Radionuclide
  • 14C (19)
  • 35S (10)
  • 3H (29)
Radioisotope
  • C-14 (19)
  • H-3 (29)
  • S-35 (10)
Volume
  • 0-50 (20)
  • 201-250 (17)

"Reporter Gene" 1-50 of 64 Products and Services

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Methionine, L-[35S], stabilized

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, and contains β-mercaptoethanol.

Carnitine HCl, L-[Methyl-3H]

Acetyl-L-carnitine HCL is a mitochondrial metabolite that facilitates the movement of fatty acids into the mitochondria for energy and is also used to generate acetyl coenzyme A.

Methylaminoisobutyric Acid, α-[1-14C]-

50 µCi quantities of α-[1-14C]-Methylaminoisobutyric Acid are available for your research.

Arginine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Arginine are available for your research.

Glutamic Acid, L-[3,4-3H]

250 µCi quantities of L-[3,4-3H]-Glutamic Acid are available for your research.

Adenosyl-L-Methionine, S-[methyl-14C]-

10 µCi quantities of S-[methyl-14C]-Adenosyl-L-Methionine are available for your research.

L-[1-14C]-Ornithine,>97%

50 µCi quantities of L-[1-14C]-Ornithine are available for your research.

Leucine, L-[14C(U)]-, Specific Activity>300mCi (11.1GBq)/mmo

50 µCi quantities of L-[14C(U)]-Leucine are available for your research.

L-[35S]-Methionine, Specific Activity:>800Ci (29.6TBq)/mMole, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.
britelite plus Reporter Gene Assay kit

britelite plus Reporter Gene Assay System

Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells.

[Glycine-2-3H]-Glutathione

50 µCi quantities of tritiated Glycine are available for your research.

Tryptophan, L-[side chain-3-14C]-,>97%

10 µCi quantities of L-[side chain-3-14C]-Tryptophan are available for your research.

Lysine, L-[4,5-3H(N)]

250 µCi quantities of L-[4,5-3H(N)]-Lysine are available for your research.

Alanine, L-[3-3H]-, 250µCi (9.25MBq)

250 µCi quantities of L-[3-3H]-Alanine are available for your research.

Arginine Monohydrochloride L-[2,3,4-3H]

250 µCi quantities of L-[2,3,4-3H]- Arginine Monohydrochloride are available for your research.

ASPARTIC ACID, L-[U-14C]

50 µCi quantities of L-[14C(U)]-Aspartic acid are available for your research.

EasyTag™ EXPRESS35S Protein Labeling Mix, [35S]-

Mixture containing both 35S-L-methionine and 35S-L-cysteine, for cell labeling. This mixture is an EasyTag formulation, meaning it can be stored at 4 °C.

Lysine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Lysine are available for your research.

Proline, L-[2,3,4,5-3H]

250 µCi quantities of L-[2,3,4,5-3H]-Proline are available for your research.

[35S]-Glutathione, 10mM DTT

Glutathione (GSH) labeled with 35S.

Glutamine, L-[3,4-3H(N)]

250 µCi quantities of L-[3,4-3H(N)]-Glutamine are available for your research. 
neolite Reporter Gene Assay kit

neolite Reporter Gene Assay System

Glow luminescence reporter gene assay combining benefits of both bright signal and long-lasting signal for firefly luciferase.

Glycine, [14C(U)]

50 µCi quantities of L-[14C(U)]-Glycine are available for your research.

Methionine, Cell Labeling Grade, L-[35S]-,>600Ci (22.2TBq)mmol, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.

Leucine, L-[3,4,5-3H(N)], Specific Activity: 100 to 150 Ci/mmol

1 mCi quantities of L-[3,4,5-3H(N)]-Leucine are available for your research.

L-[4,5-3H(N)]-Leucine, Specific Activity 40-60Ci (1.48-2.22TBq)/mmol

250 µCi quantities of tritiated L-leucine are available for your research.

ASPARTIC ACID, L-[2,3-3H]-

1 mCi quantities of L-[2,3-3H]-ASPARTIC ACID are available for your research.
twinlite dual luciferase assay system

twinlite Firefly and Renilla Luciferase Reporter Gene Assay System

twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.

L-[Methyl-3H]-Methionine

250 µCi quantities of L-[Methyl-3H]-Methionine are available for your research.

Isoleucine, L-[U-14C]

50 µCi quantities of L-[14C(U)]-Isoleucine are available for your research.

Adenosyl-L-Methionine, S-[Methyl-3H] (SAM[3H])

Tritiated SAM is commonly used in epigenetic methylation assays.

Alanine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Alanine are available for your research.

Serine, L-[3H(G)]

5 mCi quantities of L-[3H(G)]-Serine are available for your research

L-[35S]-Methionine, High radioactive concentration, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation is provided at high radioactive concentration, and contains β-mercaptoethanol.

Glycine, [2-3H]

1 mCi quantities of Glycine, [2-3H]- are available for your research.

Phenylalanine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Phenylalanine are available for your research.

SERINE, L-[3-14C]-

50 µCi quantities of [14C(U)]-serine are available for your research.

Proline, L-[2,3-3H]

250 µCi quantities of L-[2,3-3H]-Proline are available for your research.

Cysteine L-[35S]-, 10mM DTT

L-cysteine amino acid, labeled with 35S

GLUTAMINE, L-[14C(U)]-

50 µCi quantities of L-[14C(U)]-Glutamine are available for your research.

Aminohippuric Acid p-[Glycyl-2-3H]-, 1mCi (37MBq)

1 mCi quantities of aminohippouric acid are available for your research.

Aminobutyric Acid (GABA) γ-[2,3-3H(N)]-, Specific Activity: 25-40 Ci/mmol

250 µCi quantities of Aminobutyric Acid (GABA), γ-[2,3-3H(N)]- are available for your research.

Proline, L-[14C(U)], Specific Activity > 250 mCi/mmol

50 µCi quantities of L-[14C(U)]-Proline are available for your research.

Kynurenine, L-[Phenyl-3,5-3H]

50 µCi quantities of tritiated L-Kynurenine are available for your research.

Aspartic Acid, D-[2,3-3H]

250 µCi quantities of D-[2,3-3H]-Aspartic Acid are available for your research.

EasyTag™ L-[35S]-Methionine, Stabilized Aqueous Solution

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, contains β-mercaptoethanol, and has a blue dye to aid with pipetting.

Adenosyl-L-Methionine, S-[methyl-3H]-, Specific Activity: 55-85 Ci/mmol

Tritiated SAM is commonly used in epigenetic methylation assays.

Methionine, L-[35S]-, Specific Activity:>1000Ci (37.0TBq)/mMole, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol, and was developed for in vitro translation.

"Reporter Gene" 1-50 of 64 Products and Services