PerkinElmer

Reporter Gene

OF

For cost-effective automated screening and batch plate processing our luciferin-based reporter gene systems produce a long-lived glow type signal with a half-life of several hours.

  • Reporter gene assays with easily measurable phenotypes
  • Characterize the strength of promoters and enhancers
  • Define the role of transcription factors
  • Assess transfection efficiency
  • Convenient one-step processing: No washes required -- simply mix, incubate and read
  • Strong signal for remarkably accurate and thorough firefly luciferase reading
  • Enhanced stability: All our luciferase-based reagents are designed for extended storage at 2-8°C
  • No DTT: Unlike most luciferase detection reagents, ours do not contain DTT, eliminating toxic hazards and the need for hood work
  • Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
  • Excellent Z' Values: All systems offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

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Application
  • Molecular Biology (10)
Assay points(200-50,000)
  • 100 (3)
  • 1000 (3)
  • 10000 (1)
Automation Compatible
  • Yes (6)
Buffer
  • Aqueous (4)
  • Ethanol : water (1)
  • Ethanol:water (1)
  • HCl (1)
  • Sulfuric acid:ethanol (2)
  • Tricine (2)
  • Tricine with stabilizer and dye (1)
Concentration
  • 0.02 mCi/mL (2)
  • 0.05 mCi/mL (5)
  • 0.1 mCi/mL (12)
  • 0.55 mCi/mL (2)
  • 1 mCi/mL (26)
  • 10.2 mCi/mL (1)
  • 10.25 mCi/mL (1)
  • 11 mCi/mL (5)
  • 2 mCi/mL (1)
  • 31.5 mCi/mL (1)
  • 43.3 mCi/mL (1)
  • 5 mCi/mL (1)
Detection Method
  • Luminescence (6)
  • Radiometric (57)
Experimental Type
  • In vitro (6)
Format
  • Microplates (6)
Label Position
  • Specifically Labeled (44)
  • Uniformly Labeled (12)
Product Brand Name
  • britelite (1)
  • EasyTag (1)
  • NEN Radiochemicals (45)
  • neolite (1)
  • sensilite (2)
  • steadylite plus (1)
  • twinlite (2)
Radionuclide
  • 14C (19)
  • 35S (10)
  • 3H (29)
Radioisotope
  • C-14 (19)
  • H-3 (29)
  • S-35 (10)
Volume
  • 0-50 (20)
  • 201-250 (17)

"Reporter Gene" 1-50 of 64 Products and Services

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Lysine, L-[4,5-3H(N)]

250 µCi quantities of L-[4,5-3H(N)]-Lysine are available for your research.

[Glycine-2-3H]-Glutathione

50 µCi quantities of tritiated Glycine are available for your research.

Glutamic Acid, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Glutamate are available for your research.

Cysteine L-[35S]-, 10mM DTT

L-cysteine amino acid, labeled with 35S

L-[4,5-3H(N)]-Leucine, Specific Activity 40-60Ci (1.48-2.22TBq)/mmol

250 µCi quantities of tritiated L-leucine are available for your research.

Proline, L-[2,3,4,5-3H]

250 µCi quantities of L-[2,3,4,5-3H]-Proline are available for your research.
britelite plus Reporter Gene Assay kit

britelite plus Reporter Gene Assay System

Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells.

Glutamic Acid, L-[3,4-3H]

250 µCi quantities of L-[3,4-3H]-Glutamic Acid are available for your research.
Sensilite reporter gene assay kit, 1,000 data points

sensilite Enhanced Flash Luminescence (Firefly Luciferase) Reporter Gene Assay System

sensilite is an Enhanced Flash Firefly luciferase detection assay system designed to detect and quantitate Firefly luciferase from cultured cells with optimum sensitivity. The assay requires the use of a luminometer equipped with a dispenser as the signal has to be read shortly after dispensing.
PerkinElmer

Passive Lysis Solution (PLS) 1X, 100 mL

The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. It is provided in the twinlite and Sensilite kits, but if additional volume of the lysis solution is required, it is also available as a separate item, 100 mL volume per bottle.
steadylite plus Reporter Gene Assay kit

steadylite plus Reporter Gene Assay System

Reporter gene assay with longest-lasting signal for quantitation of firefly luciferase in mammalian cells.

Tyrosine, L-[Ring-3,5-3H]

250 µCi quantities of tritiated tyrosine are available for your research.

Aminobutyric Acid (GABA) γ-[2,3-3H(N)]-, Specific Activity: 25-40 Ci/mmol

250 µCi quantities of Aminobutyric Acid (GABA), γ-[2,3-3H(N)]- are available for your research.

Leucine, L-[14C(U)]-, Specific Activity>300mCi (11.1GBq)/mmo

50 µCi quantities of L-[14C(U)]-Leucine are available for your research.

EasyTag™ L-[35S]-Methionine, Stabilized Aqueous Solution

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, contains β-mercaptoethanol, and has a blue dye to aid with pipetting.

Proline, L-[2,3-3H]

250 µCi quantities of L-[2,3-3H]-Proline are available for your research.

Arginine Monohydrochloride L-[2,3,4-3H]

250 µCi quantities of L-[2,3,4-3H]- Arginine Monohydrochloride are available for your research.

Lysine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Lysine are available for your research.

TRYPTOPHAN, L-[5-3H(N)]

1 mCi quantities of L-[5-3H(N)]-Tryptophan are available for your research.

Aminohippuric Acid p-[Glycyl-2-3H]-, 1mCi (37MBq)

1 mCi quantities of aminohippouric acid are available for your research.
Twinlite dual reporter assay kit, 1,000 data points

twinlite Firefly and Renilla Luciferase Reporter Gene Assay System

twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing.

Leucine, L-[3,4,5-3H(N)], Specific Activity: 100 to 150 Ci/mmol

1 mCi quantities of L-[3,4,5-3H(N)]-Leucine are available for your research.

Kynurenine, L-[Phenyl-3,5-3H]

50 µCi quantities of tritiated L-Kynurenine are available for your research.

L-[1-14C]-Ornithine,>97%

50 µCi quantities of L-[1-14C]-Ornithine are available for your research.

L-[35S]-Methionine, Specific Activity:>800Ci (29.6TBq)/mMole, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.

Tryptophan, L-[side chain-3-14C]-,>97%

10 µCi quantities of L-[side chain-3-14C]-Tryptophan are available for your research.

Leucine, L-[3,4,5-3H(N)]-, Specific Activity: 100 to 150 Ci/mmol

250 µCi quantities of L-[3,4,5-3H(N)]-Leucine are available for your research.

Arginine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Arginine are available for your research.

Aminohippuric Acid, P-[Glycyl-1-14C]-,>97%

50 µCi quantities of P-[Glycyl-1-14C]-Aminohippuric Acid are available for your research.

Serine, L-[3H(G)]

5 mCi quantities of L-[3H(G)]-Serine are available for your research

Methionine, L-[35S], stabilized

Methionine amino acid labeled with 35S. This formulation is Tricine-stabilized, and contains β-mercaptoethanol.

Methylaminoisobutyric Acid, α-[1-14C]-

50 µCi quantities of α-[1-14C]-Methylaminoisobutyric Acid are available for your research.

Proline, L-[14C(U)], Specific Activity > 250 mCi/mmol

50 µCi quantities of L-[14C(U)]-Proline are available for your research.

Alanine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Alanine are available for your research.

Carnitine HCl, L-[Methyl-3H]

Acetyl-L-carnitine HCL is a mitochondrial metabolite that facilitates the movement of fatty acids into the mitochondria for energy and is also used to generate acetyl coenzyme A.

Alanine, L-[3-3H]-, 250µCi (9.25MBq)

250 µCi quantities of L-[3-3H]-Alanine are available for your research.

Tyrosine, L-[U-14C]

Tyrosine is an amino acid used in various protein and receptor studies.

Aspartic Acid, D-[2,3-3H]

250 µCi quantities of D-[2,3-3H]-Aspartic Acid are available for your research.

Methionine, Cell Labeling Grade, L-[35S]-,>600Ci (22.2TBq)mmol, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation contains β-mercaptoethanol.

Glutamine, L-[3,4-3H(N)]

250 µCi quantities of L-[3,4-3H(N)]-Glutamine are available for your research. 

Aminobutyric Acid (GABA), γ-[2,3-3H(N)]-, Specific Activity: 70-100 Ci/mmol

250 µCi quantities of Aminobutyric Acid (GABA), γ-[2,3-3H(N)]- are available for your research.

Glycine, [14C(U)]

50 µCi quantities of L-[14C(U)]-Glycine are available for your research.

EasyTag™ EXPRESS35S Protein Labeling Mix, [35S]-

Mixture containing both 35S-L-methionine and 35S-L-cysteine, for cell labeling. This mixture is an EasyTag formulation, meaning it can be stored at 4 °C.

L-[35S]-Methionine, High radioactive concentration, 50mM Tricine, 10mM BME

Methionine amino acid labeled with 35S. This formulation is provided at high radioactive concentration, and contains β-mercaptoethanol.

Adenosyl-L-Methionine, S-[methyl-3H]-, Specific Activity: 55-85 Ci/mmol

Tritiated SAM is commonly used in epigenetic methylation assays.

Phenylalanine, L-[14C(U)]

50 µCi quantities of L-[14C(U)]-Phenylalanine are available for your research.

L-[Methyl-3H]-Methionine

250 µCi quantities of L-[Methyl-3H]-Methionine are available for your research.

Adenosyl-L-Methionine, S-[Methyl-3H] (SAM[3H])

Tritiated SAM is commonly used in epigenetic methylation assays.

"Reporter Gene" 1-50 of 64 Products and Services