PerkinElmer

Protein Detection

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Protein detection and quantification is required for the development of protein-based therapeutics, as well as research into cell signaling, growth factors, and a variety of disease states. Our protein detection assays allow you to optimize performance, simplify workflows, and increase throughput.

We offer a broad range of protein detection assays including:

  • Traditional western blot protein detection reagents and consumables
  • Radioimmunoassay (RIA)
  • ELISA, and ELISA alternative assays
  • Tissue imaging systems and immunohistochemistry reagents
  • Innovative microfluidic technology.

Protein detection is essential for drug discovery and development, and therefore must be conducted with a high degree of accuracy and precision. Our products enable:

  • Sensitive and reliable detection with western blotting, the gold standard of protein detection
  • Fast, highly sensitive protein quantitation in a variety of sample types through use of simple, pre-validated kits and multimode plate readers in singleplex or multiplex ELISA and ELISA alternative formats
  • Amplified signal detection of proteins in tissue samples with our singleplexing and multiplexing immunohistochemistry (IHC) reagents
  • Sample acquisition times less than 40 seconds (96 samples in less than an hour) in our leading microfluidics platform virtually eliminate throughput bottlenecks and improve efficiency

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"Protein Detection" 1-50 of 1131 Products and Services

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16065 PE DropletQuant

DropletQuant, UV-VIS Spectrophotometer

The DropletQuant complements PerkinElmer's existing LabChip microfluidics instrument portfolio, which provide electrophoretic separation and analysis of nucleic acids, proteins and glycan profiling.

Key Applications:

  • DNA, RNA and protein microvolume quantitation
  • Impurity analysis; removal of buffer and contaminants from calculations of biomolecules of interest using cDrop™ software
  • Purity calculations
  • Measurement of dye labeling efficiency of microarray samples
  • Color measurements (Bradford Assay)
  • General UV/VIS measurements

BACE-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-1β (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-1ß (IL-1ß) Detection Kit is designed for detection and quantitation of mouse IL-1ß in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

Collagen IV (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human collagen IV enables the quantitative determination of collagen IV in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLisa Analyte 2

BACE-1 (human), lyophilized (AlphaLISA)

Human BACE-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human BACE-1 Detection Kit, but can also be ordered separately.
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

PIP Collagen (human) AlphaLISA Detection Kit

The AlphaLISA® Human PIP Detection Kit is designed for detection and quantitation of Human Procollagen Type I C-peptide (PIP) in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates using a homogeneous (no-wash steps, no separation steps) assay.
AlphaLISA assay principle

Cardiac Troponin T (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cardiac troponin T enables the quantitative determination of cardiac troponin T in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

E.coli HCP LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra E. coli Host Cell Proteins Detection Kit is designed for detection and quantitation of E.coli HCP in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

BRCA2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human breast cancer type 2 susceptibility protein (BRCA2) enables the quantitative determination of human BRCA2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

Claudin 7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Alpha_Strep-Tactin_Donor_bead

Strep-Tactin® Alpha Donor beads

Alpha Donor beads conjugated to Strep-Tactin®. These beads can be used to capture Strep-tag® II-tagged proteins for Alpha no-wash assays.
Protein

Osteoprotegerin (human), lyophilized (AlphaLISA)

Human osteoprotegerin standard, for use in running standard curves in AlphaLISA assays.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
AlphaLISA immunoassay kits

PTEN (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PTEN enables the detection and quantitation of human PTEN in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps). Other species of PTEN were not tested, but detection is expected with mouse and rat PTEN due to >99% sequence homology.
AlphaLISA immunoassay kits

IgE (mouse) isotyping AlphaLISA Detection Kit

This AlphaLISA® immunoassay kit enables the isotyping of mouse IgE antibodies in different sample matrices, including cell culture media. The IgE subtype plays a role in allergic reactions, parasitic infections and hypersensitivity reactions.
AlphaLISA immunoassay kits

IFN-λ2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-λ2) enables the quantitative determination of human IFN-λ2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
alphascreen surefire total

AlphaScreen SureFire Total NFkB p65 Assay Kit

The AlphaScreen® SureFire® total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
PerkinElmer

Biotinylated human FCGR3A (176Phe) (LANCE)

Biotinylated human FCGR3A/CD16a(176Phe/F158) for use in LANCE Ultra TR-FRET Fc-receptor binding assays.
SureFire principle

AlphaScreen SureFire Phospho-beta catenin (Ser45) Assay Kit

The AlphaScreen® SureFire® p-β-catenin (Ser45) assay is a sandwich immunoassay for quantitative detection of phospho-β-catenin (phosphorylated on Ser45) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Detection Kit

The AlphaLISA Mouse TNFα Detection Kit is designed for the quantitative determination of mouse TNFα in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
SureFire principle

Phospho-ERK (Thr202/Tyr204) Monoclonal AlphaScreen SureFire Assay kit

The AlphaScreen® SureFire® p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

IL-6 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-6 (IL-6) Detection Kit is designed for detection and quantitation of mouse IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit is a superior alternative to a traditional mouse IL-6 ELISA kit due to its simple and fast protocol, wide dynamic range, and high sensitivity.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
AlphaPlex-545-antibody-bead2

Anti-unmodified H3K4 AlphaPlex-545 Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Protein Reagent Kit

Protein Express Assay 25mL Sample Buffer (bulk)

1 - 25 mL of bulk buffer for the LabChip GXII and LabChip GXII Touch HT Protein Express LabChip assay.
AlphaLisa Analyte 2

α-tubulin standard, liquid (AlphaLISA)

Human α-tubulin standard, liquid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA α-tubulin Detection Kit, but can be ordered separately.
AlphaLISA immunoassay kits

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
PerkinElmer

Antifluorescein-AP Conjugate

Anti-fluorescein antibody conjugated to alkaline phosphatase
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
AlphaLISA immunoassay kits

PSMA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

Anti-6xHis AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-His antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine regions at the amino- or carboxy- terminus. These beads can be used to capture His-tagged proteins and peptides for AlphaLISA no-wash assays.

Aβ(1-40) (human) High Specificity LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Aβ(1-40) High Specificity Detection Kit is designed for detection and quantitation of human Aβ(1-40) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLisa Analyte 2

IFN-λ2 (human), lyophilized (AlphaLISA)

Human interferon lambda-2 (IFN-λ2) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human IFN-λ2 Detection Kit, but can also be ordered separately.

Glutathione AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.

PON-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PON-2 enables the quantitative determination of human Paraoxonase 2 (PON-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire CHK2 (p-Thr68) Assay Kit 

The AlphaScreen® SureFire® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

IFN-α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon-alpha (IFN-α) Detection Kit is designed for detection and quantitation of human IFN-α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The analyte in this kit consists of the IFN-α 2b subtype.

AlphaScreen FLAG (M2) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 

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