PerkinElmer

Protein Detection

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Protein detection and quantification is required for the development of protein-based therapeutics, as well as research into cell signaling, growth factors, and a variety of disease states. Our protein detection assays allow you to optimize performance, simplify workflows, and increase throughput.

We offer a broad range of protein detection assays including:

  • Traditional western blot protein detection reagents and consumables
  • Radioimmunoassay (RIA)
  • ELISA, and ELISA alternative assays
  • Tissue imaging systems and immunohistochemistry reagents
  • Innovative microfluidic technology.

Protein detection is essential for drug discovery and development, and therefore must be conducted with a high degree of accuracy and precision. Our products enable:

  • Sensitive and reliable detection with western blotting, the gold standard of protein detection
  • Fast, highly sensitive protein quantitation in a variety of sample types through use of simple, pre-validated kits and multimode plate readers in singleplex or multiplex ELISA and ELISA alternative formats
  • Amplified signal detection of proteins in tissue samples with our singleplexing and multiplexing immunohistochemistry (IHC) reagents
  • Sample acquisition times less than 40 seconds (96 samples in less than an hour) in our leading microfluidics platform virtually eliminate throughput bottlenecks and improve efficiency

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"Protein Detection" 1-50 of 1131 Products and Services

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16065 PE DropletQuant

DropletQuant, UV-VIS Spectrophotometer

The DropletQuant complements PerkinElmer's existing LabChip microfluidics instrument portfolio, which provide electrophoretic separation and analysis of nucleic acids, proteins and glycan profiling.

Key Applications:

  • DNA, RNA and protein microvolume quantitation
  • Impurity analysis; removal of buffer and contaminants from calculations of biomolecules of interest using cDrop™ software
  • Purity calculations
  • Measurement of dye labeling efficiency of microarray samples
  • Color measurements (Bradford Assay)
  • General UV/VIS measurements

IFNβ (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interferon β (IFNβ) enables the quantitative determination of porcine IFNβ in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

IL-4 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 4 (IL-4) enables the quantitative determination of porcine IL-4 in cell culture media and serum using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

Testosterone AlphaLISA Detection Kit

The AlphaLISA® Testosterone Research Kit is designed for detection and quantitation of testosterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Alpha-Unconjugated-Donor-bead

AlphaScreen Unconjugated Donor Beads

Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.

4-1BBL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human 4-1BBL enables the quantitative determination of 4-1BB ligand in buffer, cell culture media, cell supernatants, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Detection Kit

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3.

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p70 S6K (Thr389) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p70 S6K (Thr389) in a multiplexed assay.
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

IFNα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interferon α (IFNα) enables the quantitative determination of porcine IFNα in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
AlphaLISA immunoassay kits

BACE-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human BACE-1 enables the quantitative determination of human BACE-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
LANCE_Fc_receptor

FCGR3A/CD16a (176Val/V158) LANCE Ultra TR-FRET Binding Kit

This kit is designed for the quantitative determination of Human FCGR3A/CD16a (176Val/V158) and human Fc fragment binding using a homogeneous (no wash) LANCE Ultra TR-FRET binding assay.
AlphaLisa Analyte 2

IL-13 (bovine), lyophilized (AlphaLISA)

Bovine IL-13 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Bovine IL-13 Detection Kit, but can also be ordered separately.
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Anti-Human IgG3 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
LANCE Detection Kit

c-Myc (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.
AlphaLISA immunoassay kits

TGF-β1 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human TGF-β1 Biotin-Free Detection Kit is designed for the quantitative detection of human TGF-β1 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-MEK1 (Ser218/222) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-MEK1 (Ser218/222) in a multiplexed assay.
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-Ribosomal Protein S6 (Ser240/244) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-Ribosomal Protein S6 (Ser240/244) in a multiplexed assay.

LANCE Ultra ULight™-Streptavidin

Streptavidin labeled with ULight™ dye.
AlphaPlex-545-antibody-bead2

Anti-Mouse IgG AlphaPlex-545 (Tb) Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.

Anti-Chicken IgY AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-chicken IgY antibody. The antibody is a rabbit polyclonal antibody that is Fc-specific. These beads can be used to capture chicken IgY antibodies for AlphaLISA no-wash assays.
AlphaLisa Analyte 2

S100A4/FSP1 (human), lyophilized (AlphaLISA)

Human S100A4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human S100A4 Detection Kit, but can also be ordered separately.
LANCE Detection Kit

PTEN (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PTEN Detection Kit is designed for detection and quantitation of human PTEN in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

IL-1RA (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
AlphaLISA immunoassay kits

MMP-9 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
AlphaLisa Analyte 2

CD276 (human), lyophilized (AlphaLISA)

Human CD276 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human CD276 Detection Kit, but can also be ordered separately.
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

Anti-Human IgG1 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG1. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-AKT (Ser473) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-AKT (Ser473) in a multiplexed assay.
AlphaLisa Analyte 2

DNA (liquid), AlphaLISA

DNA standard for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Host Cell Residual DNA kit, but can be ordered separately.
alphascreen surefire total

AlphaScreen SureFire JNK 123 Total Assay Kit

The AlphaScreen® SureFire® total JNK1/2/3 assay is a sandwich immunoassay for quantitative detection of JNK1/2/3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLaISA® Human Factor VIII Biotin-Free Detection Kit is designed for the quantitative detection of human Factor VIII in buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-NF-κB p65 (Ser536) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-NF-κB p65 (Ser536) in a multiplexed assay.
AlphaLISA immunoassay kits

PD-L2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PD-L2 enables the quantitative determination of human programmed death 1 ligand 2 (PD-L2) in serum and over-expressing cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT1 (Ser727) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT1 (Ser727) in a multiplexed assay.
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
AlphaLisa Analyte 2

TNFR1 (mouse, soluble), lyophilized (AlphaLISA)

Mouse soluble tumor necrosis factor receptor 1 (TNFR1) standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA mouse TNFR1 detection kit, but can also be ordered separately.

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