PerkinElmer

In Situ Hybridization

OF

Reliably detect and quantitate many nucleic acids that cannot be measured with standard methods using in situ hybridization. Our reagent kits are widely cited for detection of DNA, mRNA and miRNA sequences, with some researchers reporting detection of single copies.

Signal amplification with our kits leads to reliable results, even with short synthetic oligonucleotide probes. These can be used with any hapten labeled probe for fluorescent or chromogenic signal generation.

Advantages of using In Situ Hybridization:

  • Improved limit of detection and quantitative range
  • Reduced exposure time for faster imaging
  • Excellent localization of signal to the target

Tumor Microenvironment Opal 7 Color Image
Tumor Microenvironment Opal 7 Color Image

Stain

Simultaneously detect six biomarkers plus DAPI within a single tissue section. Similar to standard immunohistochemistry, our advanced method is accessible to many laboratories where IHC method development is performed. It allows researchers to use the best primary antibodies, even those raised in the same species, together for multiplex detection. Our staining solutions provide researchers with tools to assess multiple cellular phenotypes at the same time while retaining context provided within tissue sections.
Vectra® Polaris™ Automated Quantitative Pathology Imaging System
Vectra® Polaris™ Automated Quantitative Pathology Imaging System

Image

Our quantitative pathology imaging systems deliver high quality data where morphological context is preserved down to the sub-cellular level, removing autofluorescence and increasing signal-to-noise. These systems detect and measure multiple biomarkers, even if overlapping, within a single IHC or IF tissue section or TMA. Low signal levels that were previously undetected can now be captured, and overall quantification is vastly improved - particularly for solid, formalin-fixed, and paraffin-embedded tissues.
inform Image Analysis Software for Digital Pathology
inform Image Analysis Software for Digital Pathology

Analyze

Our software programs combine the latest technologies with algorithms and intuitive, easy-to-use interfaces, giving you the power to make new, exciting discoveries from your data. Our automated advanced image analysis software allows you to visualize, analyze, quantify and phenotype cells labeled with multiple biomarkers in situ in FFPE tissue sections.
Contract Research Services for Phenotyping In Situ
Contract Research Services for Phenotyping In Situ

Contract Research Services

Interested in testing the Phenoptics™ workflow? Our Research Services team can perform multiplexed IHC for up to seven colors (six markers plus DAPI) to help you get results.
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Application
  • Immunohistochemistry (IHC) (25)
  • In situ hybridization (ISH) (25)
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Buffer
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Concentration
  • 1 mm (1)
  • 12.5 mCi/mL (1)
Detection Method
  • Chromogenic (6)
  • Fluorescence (68)
  • Radiometric (11)
Fluorophore
  • Coumarin (1)
  • Cy3 (19)
  • Cy3.5 (1)
  • Cy5 (16)
  • Cy5.5 (1)
  • Fluorescein (17)
  • Lissamine (1)
  • R110 (3)
  • R6G (2)
  • ROX (2)
  • TAMRA (4)
  • Texas Red (3)
  • TMR (5)
Format
  • Slides (25)
Label Position
  • alpha (2)
Nucleotide
  • ATP (2)
  • CTP (4)
  • dATP (6)
  • dCTP (10)
  • ddATP (6)
  • ddCTP (6)
  • ddGTP (6)
  • ddUTP (6)
  • dGTP (4)
  • dUTP (9)
  • GTP (2)
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Product Brand Name
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Radionuclide
  • 35S (2)
Radioisotope
  • S-35 (2)
Type
  • Opal 520 (1)
  • Opal 540 (1)
  • Opal 570 (1)
  • Opal 620 (1)
  • Opal 650 (1)
  • Opal 690 (1)
Volume Capacity
  • 150 µL (2)

"In Situ Hybridization" 1-50 of 102 Products and Services

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PerkinElmer

Opal Multiplex Manual IHC Kit 50 slides

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.
PerkinElmer

Biotin-11-ddCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
PerkinElmer

Cyanine 5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
Opal 650 Reagent Pack

Opal Reagent Packs

Opal is a practical workflow for simultaneous detection of up to 6 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed.
PerkinElmer

TSA Cyanine 3 Reagent Pack

TSA® Cyanine 3 (Cy3) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra Cy3 in the localized area of the probe or antibody.
PerkinElmer

R110-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Cyanine 5-UTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

TSA Biotin Reagent Pack

TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
PerkinElmer

Fluorescein-12-GTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
TSA Fluorescein System

TSA Fluorescein System

TSA® fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
PerkinElmer

Biotin-11-ddUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
TSA Cyanine 3 System

TSA Cyanine 3 System, for 50-150 Slides

TSA® Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
PerkinElmer

Cyanine 5-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

TSA Plus DNP (AP)

TSA® Plus DNP (AP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP alkaline phosphatase, with appropriate substrate.

TSA Plus Cyanine 5.5

TSA® Plus Cyanine 5.5 (Cy5.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5.5 in the localized area of the probe or antibody.
PerkinElmer

Biotin-11-dGTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

R6G-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

R110-ddCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

UTPαS, [35S]- 1250Ci/mmol, 12.5mCi/ml

Uridine triphosphate, labeled on the alpha phosphate group with 35S.
PerkinElmer

Cyanine 5-dGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

R110-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Fluorescein-12-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
TSA -Plus Cyanine 3 System

TSA Plus Cyanine 3

TSA® Plus Cyanine 3 (Cy3) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3 in the localized area of the probe or antibody.
TSA Biotin System

TSA Biotin System

TSA® biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.
PerkinElmer

Biotin-11-dCTP 1mM 25nmol

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
Amplification Diluent

TSA Cyanine 5 Tyramide Reagent Pack, Reagents for 50-150 Slides

TSA® Cyanine 5 (Cy5) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
PerkinElmer

Cyanine 5-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
TSA Cyanine 3 & 5, TMR, Fluorescein Evaluation Kit

TSA Cy 3, Cy 5, TMR, Fluorescein Evaluation Kit

TSA® Plus multi-fluorophore detection kit, for amplification of multiple signals in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols.
PerkinElmer

Cyanine 3-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Biotin-11-dUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
PerkinElmer

Fluorescein-12-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Cyanine 3-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Cyanine 3-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Texas Red®-5-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
TSA Cyanine 5 System

TSA Cyanine 5 System, for 50-150 Slides

TSA® Cyanine 5 (Cy5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy5 in the localized area of the probe or antibody.
TSA -Plus Fluorescein System

TSA Plus Fluorescein

TSA® Plus fluorescein detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra fluorescein in the localized area of the probe or antibody.
PerkinElmer

Cyanine 5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

Fluorescein-12-CTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

Cyanine 5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Biotin-11-GTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

Texas Red®-5-dCTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
Amplification Diluent

TSA Tetramethylrhodamine Tyramide Reagent Pack, Reagents for 100-300 Slides

TSA® tetramethylrhodamine (TMR) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.

Texas Red-5-dUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
TSA Tetramethylrhodamine System

TSA Tetramethylrhodamine System, for 100-300 Slides

TSA® tetramethylrhodamine (TMR) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra TMR in the localized area of the probe or antibody.
PerkinElmer

Cyanine 3-dATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Cyanine 3-CTP, 100 nmol

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Biotin-16 UTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
PerkinElmer

TAMRA-ddUTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Cyanine 5-ddGTP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
PerkinElmer

Fluorescein-12-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

"In Situ Hybridization" 1-50 of 102 Products and Services

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