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LANCE Ultra ULight-TK peptide, 1,000 assay points

ULight™-labeled synthetic peptide containing 28 amino acid peptide with 8 Tyrosine residues in different amino acid context that broadly provides for the recognition specificity of greater than 85% of 83 tested cytoplasmic and receptor tyrosine kinases.

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Product Number Unit Size List Price Your Price Quantity
TRF0127-D 0.5 nmol 368.00 USD
TRF0127-M 5 nmol 1086.00 USD

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LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points

  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore ULight
Molecular Modification Phosphorylation
One Unit Contains 0.5 nmol
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 0.5 nmol

Resource Library & Events

Resource Type File Name File Format
Application Note Improved Stability of the LANCE Ultra Signal in Kinase Assays PDF 206 KB
Technical Note LANCE Ultra EphA4 Kinase Assay PDF 627 KB