Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight™-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence: CKKSRGDYMTMQIG
LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:
For ULight substrates:
In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.
Application |
Kinase
|
---|---|
Automation Compatible |
Yes
|
Brand |
LANCE Ultra
|
Detection Method |
Time-Resolved Fluorescence (TRF)
TR-FRET
|
Experimental Type |
In vitro
|
Fluorophore |
ULight
|
Format |
Microplates
|
Molecular Modification |
Phosphorylation
|
Shipping Conditions |
Shipped in Dry Ice
|
Therapeutic Area |
Metabolic
|
Unit Size |
1 nmol
|