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LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide, 5 nmoles

Glycogen synthase peptide (Sequence: PASVPPSPSLSRHSSPHQ(pS)ED) labeled with ULight™ dye. Synthetic peptide containing residues surrounding Ser641 and Ser657 of human Muscle Glycogen Synthase. This peptide is pre-phosphorylated on Ser657. Target phosphorylation site: Ser641. This peptide substrate can be phosphorylated by a variety of kinases including GSK3α and GSK3β.

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Product Number Unit Size List Price Your Price Quantity
TRF0131-D 0.5 nmol 369.00 USD
TRF0131-M 5 nmol 813.00 USD

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Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:


  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:


  • 0.5 nmoles = 1,000 assay points

  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore ULight
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 5 nmol

Resources, Events & More

Resource Type File Name File Format
Application Note Comparison of LANCE Ultra TR-FRET to PerkinElmer's Classical LANCE TR-FRET Platform for Kinase Applications PDF 805 KB
Application Note Improved Stability of the LANCE Ultra Signal in Kinase Assays PDF 206 KB
Technical Note Technical note: GSK3ß Kinase Assay PDF 1022 KB