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LANCE Ultra ULight™-CDK1 (Tyr15) Peptide, 0.5 nmole

ULight™-labeled synthetic peptide derived from amino acids 9-20 of human cyclin-dependent kinase 1 (CDK1). The ULight-CDK1 peptide can be phosphorylated at Tyr15 by Lck kinase. Peptide sequence : CAGAGKIGEGTYGVVYK

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Product Number Unit Size List Price Your Price Quantity
TRF0122-D 0.5 nmol 369.00 USD
TRF0122-M 5 nmol 1131.00 USD

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Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

 

  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

 

 

For ULight substrates:

 

 

  • 0.5 nmoles = 1,000 assay points

  • 5 nmoles = 10,000 assay points

 

 

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

 

Specifications

Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore ULight
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 0.5 nmol