PerkinElmer
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ULight-anti-V5 (LANCE TR-FRET), 20 nmol

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.

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Part Number Unit Size List Price Your Price Quantity
TRF505D 0.2 nmol 403.00 USD
TRF505M 2 nmol 1420.00 USD
TRF505R 20 nmol 10200.00 USD
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Overview

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Anti-V5
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore ULight
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 20 nmol

Resources, Events & More

All (6)
Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Guide LANCE Protein: Protein Interaction Quick Start Guide PDF  1 MB
Guide LANCE TR-FRET Immunoassay Conversion Guide PDF  3 MB
Guide LANCE TR-FRET protein-protein interaction assay development guide PDF  1 MB
Application Note Developing a LANCE TR-FRET Assay for Screening PD-1 and PD-L1 Binding Inhibitors PDF  1 MB
Technical Note Development of a Simple Bromodomain Binding Assay Using LANCE TR-FRET PDF  1 MB