PerkinElmer
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twinlite Firefly and Renilla Luciferase Reporter Gene Assay System, 100 assay kit

twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. It requires the use of a luminometer equipped with two dispensers as the signal has to be read shortly after dispensing. Please note the 1,000 assay point kit will soon be available. The 100 assay point kit size is now available.

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Part Number Unit Size List Price Your Price Quantity
6066706 100 assay points (96-well) / 400 assay points (384-well) 211.00 USD
6066709 1,000 assay points (96-well) / 4,000 assay points (384-well) 1327.00 USD
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Overview

twinlite is a dispense-and-read ultra-high sensitivity dual luciferase reporter gene assay system for the quantitation of Firefly and Renilla luciferase expression in mammalian cells. twinlite was designed to provide maximum signal intensity for assays requiring the utmost sensitivity, and is suitable for use in a tube or 96- and 384-well microplate format. twinlite is ideal for:

  • Research activities in the gene transcription regulation, miRNA, signal transduction and viral replications areas, where maximum sensitivity is needed
  • High throughput assays employing continuous processing methodology

twinlite benefits:

  • Very high assay sensitivity
  • Designed for use with luminometers equipped with dispensers
  • Suitable for use 96- and 384-well microplates readers as well as for tube-based luminometers
  • Ideal for smaller-scale sample analysis when maximum sensitivity is needed
  • Odor free
  • Exceptional stability of the reagents even after reconstitution

As with all members of our Lites suite of luciferase detection reagents, twinlite offers superior performance in an easy-to-use format, providing the following benefits:

  • Convenient Two-successive-Steps Processing: After cell lysis, simply dispense and read immediately
  • No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards and the need for hood work
  • Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z'values

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
Product Brand Name twinlite
Shipping Condition Ambient
Unit Size 100 assay points (96-well) / 400 assay points (384-well)

Tools

Resources, Events & More

Resource Type File Name File Format
Guide Twinlite Dual Luciferase Quick Start Guide PDF  757 KB
Flyer Dual Reporter Gene Assay: DTT-free method for measuring Firefly and Renilla luciferases PDF  639 KB