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TSA Plus Cyanine 3.5, evaluation kit

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.

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Part Number Unit Size List Price Your Price Quantity
NEL763E001KT 1 Kit 216.00 USD
NEL763001KT 50-150 slides 394.00 USD
NEL763B001KT 250-750 slides 1727.00 USD
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TSA Plus kits deliver ultimate sensitivity in immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH) assays. This widely referenced method uses horseradish peroxidase (HRP) to mediate intense covalent deposition of detection labels at very close proximity to the epitope or sequence of interest. TSA Plus has been employed for quantitative detection in a wide variety of sample types including formalin-fixed, paraffin-embedded (FFPE) tissue, cryosections and cultured cells.


TSA Plus Cyanine 3.5 provides another option for fluorescent detection with spectral characteristics that are generally similar to mCherry, Alexa Fluor 568® or Texas Red®. It has maximum absorbance at 581 nm and maximum emission at 596 nm.


TSA Plus Cyanine 3.5 is ideal for multiplexed IHC or ISH in combination with other TSA fluorophores. The Opal™ protocol allows simultaneous measurement of 6 or more biomarkers in FFPE tissue.



Fluorophore Cy3.5
Product Brand Name TSA Plus
Shipping Condition Blue Ice
Unit Size 1 Kit


Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure TSA™ Signal Amplification for IHC, ICC, IF PDF  3 MB
Brochure TSA™ Signal Amplification for ISH PDF  2 MB
Application Note Multiplex Biomarker Imaging - Cancer Immunology (Capturing Immune Status in FFPE Sections) PDF  1 MB
Manual Manual for TSA Plus Fluorescence Kits PDF  454 KB
Flyer Opal Multiplex Tissue Staining Flyer PDF  2 MB
Webinars Signal Amplification for Immunohistochemistry Link
Webinars Signal Amplification for In Situ Hybridization Link