TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.
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TSA Plus kits deliver ultimate sensitivity in immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH) assays. This widely referenced method uses horseradish peroxidase (HRP) to mediate intense covalent deposition of detection labels at very close proximity to the epitope or sequence of interest. TSA Plus has been employed for quantitative detection in a wide variety of sample types including formalin-fixed, paraffin-embedded (FFPE) tissue, cryosections and cultured cells.
TSA Plus Cyanine 3.5 provides another option for fluorescent detection with spectral characteristics that are generally similar to mCherry, Alexa Fluor 568® or Texas Red®. It has maximum absorbance at 581 nm and maximum emission at 596 nm.
TSA Plus Cyanine 3.5 is ideal for multiplexed IHC or ISH in combination with other TSA fluorophores. The Opal™ protocol allows simultaneous measurement of 6 or more biomarkers in FFPE tissue.
|One Unit Contains||1 each|
|Product Brand Name||TSA Plus|
|Shipping Condition||Blue Ice|
|Resource Type||File Name||File Format|
|Brochure||TSA™ Signal Amplification for IHC, ICC, IF||PDF 3 MB|
|Brochure||TSA™ Signal Amplification for ISH||PDF 2 MB|
|Application Note||Multiplex Biomarker Imaging - Cancer Immunology (Capturing Immune Status in FFPE Sections)||PDF 1 MB|
|Manual||Manual for TSA Plus Fluorescence Kits||PDF 454 KB|
|Flyer||Opal Multiplex Tissue Staining Flyer||PDF 2 MB|
|Webinar||Signal Amplification for Immunohistochemistry||Link|
|Webinar||Signal Amplification for In Situ Hybridization||Link|