PerkinElmer
check quantity

TSA Plus Biotin Kit, 50 - 150 slides

TSA® Plus Biotin detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra biotin in the localized area of the probe or antibody.

You successfully added item(s) to your cart

Part Number
Unit Size
List Price
Your Price
Quantity
NEL749A001KT
50-150 slides
368.00 USD
more
NEL749B001KT
250-750 slides
1380.00 USD
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

TSA Plus Biotin improves sensitivity in immunohistochemistry, in situ hybridization, and related experiments by up to 1000 fold while adding just 2 steps or about 20 minutes to existing protocols.

 

Features/Benefits:

 

  • 10-20 times higher sensitivity than regular TSA
  • Outstanding resolution and clarity
  • Reduce non-specific background
  • Ideal for use with low affinity antibodies
  • Conserve precious reagents by using higher dilutions of primary antibodies and probe
  • Combine with labeled streptavidin for fluorescent or chromogenic detection

Specifications

Detection Method Fluorescence, Chromogenic
Hapten Biotin
Product Brand Name TSA Plus
Shipping Condition Blue Ice
Unit Size 50-150 slides
Resources, Events & More
  • All

Application Note

Flow Cytometric Detection of pAKT and FoxP3 in Jurkat T Cells Using TSA™ Plus Signal Amplification Technology

Flow Cytometric Detection of pAKT and FoxP3 in Jurkat T Cells Using TSA™ Plus Signal Amplification Technology

PDF 4 MB
Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB

Manual

TSA™ Plus Biotin Kit Manual

TSA Plus Biotin technology uses HRP to catalyze the covalent deposition of biotin labeled amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the covalent deposition of numerous biotin labels immediately adjacent to the immobilized HRP enzyme. These labels can then be indirectly detected by standard chromogenic or fluorescent techniques, with significant enhancement of the signal.

PDF 380 KB