The AlphaLISA® Human Transferrin Detection Kit is designed for detection and quantitation of human transferrin in cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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Human Transferrin is a 679 amino acid protein (80 kDa) composed of alpha helices and beta sheets that form two distinct domains that each contain an iron binding site. This protein functions to control the level of free iron found in biological fluids. The liver is the main site of transferrin synthesis and its main role is to deliver iron to all tissues. Normal human serum transferrin levels range from 200-360 mg/dL. The transferrin protein has been implicated in several disease states including iron deficiency anemia (high serum transferrin levels) and liver toxicity. The present kit permits detection of human transferrin (i.e. analyte) in different sample matrices, including DMEM cell culture media.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||Manual AlphaLISA Human Transferrin AL311||PDF 266 KB|