APC-labeled anti-His antibody, for capturing His-tagged proteins and peptides in LANCE TR-FRET assays.
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Mouse monoclonal antibody against 6xHis polypeptide conjugated to allophycocyanin (APC). APC is a fluorescent light harvesting protein unique to cyanobacteria and red algae and a member of the phycobiliprotein family of direct fluorescent dyes. Surelight APC is a chemically and intramolecularly cross-linked form of a protein. It is produced using a proprietary manufacturing process to increase thermal stability while maintaining the fluorescent and spectral properties of native dye.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Antibody Conjugates||Anti-6X His|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE SureLight|
|Shipping Condition||Blue Ice|
|Unit Size||1 mg|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Guide||LANCE Protein: Protein Interaction Quick Start Guide||PDF 1 MB|
|Guide||LANCE TR-FRET Immunoassay Conversion Guide||PDF 3 MB|
|Guide||LANCE TR-FRET protein-protein interaction assay development guide||PDF 1 MB|
|Application Note||Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of hIgGs a||PDF 1 MB|
|Application Note||Developing a LANCE TR-FRET Assay for Screening PD-1 and PD-L1 Binding Inhibitors||PDF 1 MB|
|Technical Note||Development of a Simple Bromodomain Binding Assay Using LANCE TR-FRET||PDF 1 MB|