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SureLight Allophycocyanin-Streptavidin (APC-SA), protein binding assays, 30 mg, lyophilized

APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.

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Product Number Unit Size List Price Your Price Quantity
AD0201 1 mg 374.00 USD
AD0202 30 mg 10500.00 USD

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Overview

PerkinElmer offers two versions of SureLight APC-SA: one for protein binding assays, and one for kinase assays. SureLight APC-SA for protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve the best performance in protein binding applications.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Streptavidin
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore SureLight APC
Product Brand Name LANCE SureLight
Shipping Condition Blue Ice
Unit Size 30 mg