APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AD0201||1 mg||386.00 USD|
|AD0202||30 mg||10800.00 USD|
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PerkinElmer offers two versions of SureLight APC-SA: one for protein binding assays, and one for kinase assays. SureLight APC-SA for protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve the best performance in protein binding applications.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE SureLight|
|Shipping Condition||Blue Ice|
|Unit Size||30 mg|
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|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 77 KB|
|Guide||LANCE Protein: Protein Interaction Quick Start Guide||PDF 1 MB|
|Guide||LANCE TR-FRET Immunoassay Conversion Guide||PDF 3 MB|
|Guide||LANCE TR-FRET protein-protein interaction assay development guide||PDF 1 MB|
|Application Note||Biochemical Binding ADCC Assays Utilizing LANCE Toolbox Reagents for the Characterization of hIgGs a||PDF 1 MB|
|Application Note||Developing a LANCE TR-FRET Assay for Screening PD-1 and PD-L1 Binding Inhibitors||PDF 1 MB|
|Technical Note||Development of a Simple Bromodomain Binding Assay Using LANCE TR-FRET||PDF 1 MB|