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AlphaScreen Streptavidin Donor beads 5 mg

Alpha Donor beads conjugated to streptavidin.

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Part Number Unit Size List Price Your Price Quantity
6760002S 1 mg 349.00 USD
6760002 5 mg 1397.00 USD
6760002B 50 mg 9800.00 USD
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Overview

These beads can be used to capture biotinylated antibodies, proteins, peptides, nucleic acids, and small molecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein-DNA interaction assays
  • Protein-RNA interaction assays
  • Protein-small molecule interaction assays
  • Analyte detection assays
  • Biomarker detection assays
  • Antibody detection assays
  • Enzymatic assays
  • Other immunoassays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym ""Alpha"" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Streptavidin
Automation Compatible Yes
Bead Type or Core Bead Type Alpha Donor
Product Brand Name Alpha
Shipping Condition Blue Ice
Unit Size 5 mg

Resources, Events & More

All (18)
Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF  4 MB
Brochure Alpha product listing PDF  127 KB
Brochure Handle Large Biomolecular Interactions With Ease PDF  798 KB
Guide Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
Guide AlphaLISA immunoassay development quick guide PDF  1 MB
Guide ELISA to AlphaLISA Immunoassay Conversion Guide PDF  1 MB
Guide User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
Application Note AlphaLISA GMO Protein Detection Seed Extract PDF  1 MB
Application Note Biochemical Binding ADCC Assays Utilizing AlphaLISA Toolbox Reagents PDF  1 MB
Application Note Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction PDF  3 MB
Application Note Immunogenicity Assessment Using the AlphaLISA Technology PDF  289 KB
Technical Note AlphaLISA Assay for Measurement of Core Fucosylated Glycoproteins Using LCA Lectin PDF  1011 KB
Technical Note AlphaLISA Ligand Binding Assays for Measurement of Human Immunoglobulin G Subclasses in Animal Sera PDF  838 KB
Technical Note AlphaLISA Tau-Ser400 O-GlcNAc hydrolase (OGA) Assay PDF  650 KB
Technical Note Designing Your Own AlphaLISA Assay: Selecting Toolbox Bead Pairs to Avoid Potential Bead-Bead Intera PDF  1 MB
Poster (All) Comparison of endogenous methyl marks on histone H3 in cancer-derived cell lines using AlphaLISA PDF  1 MB
Poster (All) Tau Duplex: A novel assay to detect phosphorylated and total tau protein in neuronal cells using Alpha technology PDF  515 KB
Event Society of Laboratory Automation & Screening (SLAS) Event